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Inositol-containing Ceratonia siliqua Linn extract

The technology of extract and carob bean is applied in the field of carob bean extract and preparation thereof, and can solve problems such as immaturity of inositol extraction method

Inactive Publication Date: 2015-04-29
上海诺金科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction method of inositol is immature. Kang et al. obtained the method of purifying inositol from soybean, and studied the effect of inositol on postprandial blood sugar in type 2 diabetic patients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of Carob Extract Containing Inositol

[0018] 1) Weigh 100g of carob, wash and dry;

[0019] 2) Put carob powder in a round-bottomed flask, add 2L of distilled water, extract at 100°C for 3 hours, centrifuge, add 1.5L of distilled water to the filter residue for extraction once, centrifuge to discard the residue, combine the filtrate twice, and concentrate under reduced pressure to the original volume 1 / 30 of;

[0020] 3) Add pH 4.5 acetic acid buffer solution and 2.5% phytase concentrate, enzymolyze at 40°C for 3 hours, concentrate under reduced pressure to 1 / 5 volume, remove acetic acid, adjust pH to neutral, and obtain enzymatic hydrolysis solution ;

[0021] 4) The above enzymatic solution was subjected to column chromatography through HZ202 type anion exchange resin, the resin was washed with 500mL deionized water, the eluate was collected, the eluate was concentrated under reduced pressure, and dried at 105°C to obtain carob beans containi...

Embodiment 2

[0022] Example 2 Preparation of Carob Extract Containing Inositols

[0023] 1) Weigh 100g of carob, wash, dry, put in a grinder and grind into powder;

[0024] 2) Put the dried carob in a round bottom flask, add 2L of 80% ethanol solution, extract at 80°C for 3 hours, centrifuge, add 1.5L of 80% ethanol solution to the filter residue for extraction once, centrifuge to discard the residue, and combine twice The filtrate was concentrated under reduced pressure to 1 / 30 of the original volume;

[0025] 3) Add pH 4.5 acetic acid buffer solution and 2.5% phytase concentrate, enzymolyze at 40°C for 3 hours, concentrate under reduced pressure to 1 / 5 volume, remove acetic acid, adjust pH to neutral, and obtain enzymatic hydrolysis solution ;

[0026] 4) The above enzymatic solution was subjected to column chromatography through HZ202 type anion exchange resin, the resin was washed with 500mL deionized water, the eluate was collected, the eluate was concentrated under reduced pressu...

Embodiment 3

[0027] Example 3 Preparation of Carob Extract Containing Inositols

[0028] 1) Weigh 100g of carob, wash, dry, put in a grinder and grind into powder;

[0029] 2) Put the dried carob in a round bottom flask, add 2L of 40% ethanol solution, extract at 80°C for 3 hours, centrifuge, add 1.5L of 40% ethanol solution to the filter residue to extract once, centrifuge to discard the residue, and combine twice The filtrate was concentrated under reduced pressure to 1 / 30 of the original volume;

[0030] 3) Add pH 4.5 acetic acid buffer solution and concentrated solution 4% phytase, enzymolyze at 40°C for 3 hours, concentrate under reduced pressure to 1 / 5 volume, remove acetic acid, adjust pH to neutral, and obtain enzymatic hydrolysis solution ;

[0031] 4) The above enzymatic solution was subjected to column chromatography through HZ202 type anion exchange resin, the resin was washed with 500mL deionized water, the eluate was collected, the eluate was concentrated under reduced pr...

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PUM

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Abstract

The invention relates to an inositol-rich Ceratonia siliqua Linn extract and its preparation method. Ceratonia siliqua Linn used as a raw material undergoes extraction, enzymatic hydrolysis, column chromatography, drying and other processes to prepare the Ceratonia siliqua Linn extract. The Ceratonia siliqua Linn extract is rich in inositol. inositol contained in the Ceratonia siliqua Linn extract can be an inositol substance or an inositol monomer, wherein proportion by weight of the inositol substance is 40-60 wt%.

Description

technical field [0001] The invention relates to a carob extract containing inositol and a preparation method thereof. Background technique [0002] Carob, scientific name Ceratonia siliqua, belongs to the genus Carob in the family Carobaceae. It is native to the eastern Mediterranean and has been artificially introduced and cultivated in China. Carob not only has very high nutritional value, but also has very high medicinal value. Ripe carob can be made into powder for the food industry, and can also be used as animal feed and additives. Because carob contains natural sugar content, low in calories and fat-free, experts recommend that patients with heart disease and stomach problems can eat carob. The calcium content in carob syrup is three times that of milk, and it is rich in vitamin E and minerals. It has a good effect on treating colds and coughs, softens breasts and eliminates phlegm, and helps the stomach to work normally. [0003] Studies abroad have found that ca...

Claims

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Application Information

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IPC IPC(8): C12P7/02
CPCC12P7/02
Inventor 夏涛刘华奇李玉灵李琦谢卫军郭荣鑫
Owner 上海诺金科生物科技有限公司
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