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An arbuscular mycorrhizal fungus that efficiently degrades atrazine and promotes plant growth and its application

A technology for promoting plant growth and arbuscular mycorrhizal fungi, applied in fungi, microbial-based methods, microorganisms, etc., can solve the problems of limited biomass accumulation, achieve good results, maintain biological activity, and efficiently degrade

Active Publication Date: 2017-03-29
南京蕃茂生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem that existing arbuscular mycorrhizal fungi for repairing atrazine-contaminated soil have limited accumulation of plant biomass, and provide an arbuscular mycorrhizal fungus that can efficiently degrade atrazine and effectively promote plant growth Mycorrhizal fungi and their applications

Method used

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  • An arbuscular mycorrhizal fungus that efficiently degrades atrazine and promotes plant growth and its application
  • An arbuscular mycorrhizal fungus that efficiently degrades atrazine and promotes plant growth and its application
  • An arbuscular mycorrhizal fungus that efficiently degrades atrazine and promotes plant growth and its application

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Experimental program
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Embodiment 1

[0020] Such as Figure 1-3 As shown, an arbuscular mycorrhizal fungus that efficiently degrades atrazine and promotes plant production in this embodiment is the spore of Acaulospora lacunosa HDSF4, which is round or nearly round, and in the spore soil Solitary, growing laterally on conidia hyphae, orange-brown to dark brown, 80-130 μm in diameter; 2 layers of spore wall, W 1 It is colorless, 0.5μm thick, W 2 Orange-brown to dark brown, layered wall, the inner and outer surfaces are covered with regular or irregular conical and dish-shaped pits; the bud wall has 2 layers, the first layer is colorless membranous, 0.5 μm thick, and the second layer is colorless Membranous, with beaded patterns on the surface, 0.5-1 μm thick.

[0021] W in the spore wall of Acaulospora lacunosa (Acaulospora lacunosa) HDSF4 in the present embodiment 1 It is a perishable wall, which falls off when the spores mature, and has no reaction with Melzer's reagent; W 2 The wall thickness is 2 μm, the s...

Embodiment 2

[0025] Acaulospora lacunosa HDSF4 was screened in farmland soil contaminated by atrazine residues in Qiqihar.

[0026] In this implementation, the collection, screening and purification of Acaulospora lacunosa HDSF4 are carried out according to the following steps:

[0027] 1. Take the soil in the 2-20cm soil layer where the root circumference of the plant is polluted by the pesticide atrazine residues, use wet sieve decantation-sucrose centrifugation to separate the spores of different arbuscular mycorrhizal fungi from the soil, and separate the different arbuscular mycorrhizal fungi The root fungal spores were classified, and the dominant arbuscular mycorrhizal fungal spores were screened out; among them, the AM fungal dominance was divided into 4 grades according to the important value (I), I>50% was the dominant genus (species), 30 %<I≤50% is a subdominant genus (species), 10%<I≤30% is an associated genus (species), I≤10% is a rare genus (species);

[0028] Two, soak the ...

Embodiment 3

[0031]The expansion cultivation method of Acaulospora lacunosa (Acaulospora lacunosa) HDSF4 of the present embodiment comprises the following steps:

[0032] 1. Preparation of culture substrate: Weigh peat soil, frog stone and sand according to the volume ratio of 2:3:5, mix them evenly, and sterilize with high-temperature steam at 121°C for 1.5h;

[0033] 2. Expansion of strains: Germinate sorghum seeds, sow them on the culture substrate prepared in step 1, inoculate Acaulospora lacunosa HDSF4 strains at the same time, cover with soil 0.8-1.2cm, water and light After culturing for 4 months, the spores, mycelium fragments and host root system infected by the fungus containing Acaulospora lacunosa HDSF4 are obtained as the strains for propagation.

[0034] The spore density of Acaulospora lacunosa HDSF4 in the substrate obtained by the multiplication method of this embodiment is 20 spores / g soil.

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Abstract

An arbuscular mycorrhizal fungus capable of efficiently degrading atrazine and promoting plant growth and its application relates to a fungus and its application, in particular to an arbuscular mycorrhizal fungus of the genus Asepsis genus and its application. The method solves the problem that the existing arbuscular mycorrhizal fungi for repairing atrazine-contaminated soil have limited effect on the accumulation of plant biomass. An arbuscular mycorrhizal fungus that efficiently degrades atrazine and promotes plant growth in the present invention is Acaulospora lacunosa HDSF4, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.9907. Acaulospora lacunosa HDSF4 of the present invention is used for repairing the soil polluted by atrazine, and it has been proved by experiments that the effect is good, and can effectively increase the growth of plants in the soil polluted by atrazine .

Description

technical field [0001] The invention relates to a fungus and its application, in particular to an arbuscular mycorrhizal fungus of the genus Asepsis and its application. Background technique [0002] Atrazine, also known as atrazine, is a selective systemic conduction pre-emergence and post-emergence herbicide, suitable for corn, sorghum, orchard, woodland, etc., and can control annual grass weeds and broad-leaved weeds, It also has a certain inhibitory effect on some perennial weeds. Due to its low cost and good weed control effect, atrazine is widely used in agricultural planting. And long-term use leads to a large amount of residues in the soil, which greatly reduces the yield of crops. The remediation of atrazine-contaminated soil can use physical, chemical and biological methods, because the physical treatment method only transfers the pollutants, cannot completely eliminate atrazine, and is expensive; while the chemical treatment method is likely to cause the contami...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B09C1/10C12R1/645
CPCB09C1/105C12N1/145C12R2001/645
Inventor 宋福强孙育新王辰郭夏立常伟范晓旭
Owner 南京蕃茂生物科技有限公司
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