Method for extracting trypsin inhibitor from bamboo shoots

Abstract

The invention discloses a method for extracting a trypsin inhibitor from bamboo shoots. The method comprises the following steps: peeling the bamboo shoots, cleaning with water and slicing; adding the sliced bamboo shoots into water according to a mass volume ratio 1g:(1-5)ml of the bamboo shoots to water; grinding and reacting for 3-30min at 60-90 DEG C; filtering, removing the residue and taking the filtrate; adding a 600-750g / L ammonium sulfate solution into the filtrate while stirring until the solution is clear; standing for 8-12 hours at 3-7 DEG C; centrifuging, removing the supernate and dissolving the precipitate in water; treating through a sephadex G25 column and eluting with water; concentrating the elution fraction and treating through a sephadex G50 column and eluting with water; treating the elution fraction with diethylaminoethylcellulose-sephadex A50; performing gradient elution with a 0.1-0.3g / L NaCl liquid; and collecting 0.12-0.15g / L gradient elution fraction to obtain the trypsin inhibitor. The method disclosed by the invention can be used for developing bamboo shoot products with high added value, the economic benefit of the bamboo shoot processing industry is increased, the resource utilization efficiency is improved, and the development of bamboo industry and the prosperity of rural economy are promoted.

Description

Technical field: [0001] The invention belongs to the field of protein preparation, in particular to a method for extracting trypsin inhibitor from bamboo shoots. Background technique: [0002] my country has very rich bamboo resources, mainly distributed in Fujian, Jiangxi, Zhejiang and other provinces. Bamboo shoots are swollen buds and young stems formed by germination and differentiation of buds on bamboo whip or stalk base. It is a high-protein and low-fat forest vegetable. With the promotion of high-yield technology in bamboo shoot forests and bamboo shoot dual-purpose forests, the output of fresh bamboo shoots in my country has been increasing year by year. processing. However, in recent years, the market for traditional bamboo shoot products has become saturated and the market competition is fierce, resulting in low economic benefits of related enterprises, low raw material prices of fresh bamboo shoots, and increased production but not income of farmers. In additio...

AI Technical Summary

Problems solved by technology

However, in recent years, due to the saturation of the market for traditional bamboo shoots and fierce competition in the market, the economic benefits of related enterprises have been low, the raw material prices of fresh bamboo shoots have been low, and farmers have increased production without increasing income.

In addition, traditional bamboo shoot processing will produce a large amount of waste such as bamboo shoot heads, bamboo shoot shells, and bamboo shoot cooking liquid, resulting in waste of resources and environmental pollution.

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