Clean production process for simultaneously extracting lipid and collagen from animal fat tissues
A technology of animal fat and collagen, which is applied in the production of fat oil/fat, animal/human protein, and fat production, etc. It can solve the problems of bearish price, never pay attention to the development and utilization of connective tissue, and the poor sales situation, so as to improve the utilization degree, increased utilization value, and the effect of increased effective utilization
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example 1
[0016] Put 3000g of pig fat into the extractor, add 1000ml of a mixed solvent consisting of 2mol / L sodium hydroxide and 96% (v / v) aqueous ethanol in a ratio of 1:1, soak at 60°C for 10h, Pig fat is completely melted and layered, the upper layer is lard, and the lower layer is collagen liquid.
[0017] After the upper layer of lard was collected and cooled, 1680g of white lard was obtained. After testing, all indicators reached the national first-class lard standard.
[0018] Collect the collagen solution in the lower layer, neutralize it with hydrochloric acid to pH = 6.8, put it on a rotary evaporator, recover the alcohol at 60°C and 1kPa until the volume of the remaining liquid is 1500ml, put it into a dialysis bag and dialyze it against running tap water for 48 hours, and then put it on a rotary evaporator Concentrate to 1000ml on an evaporator at 60°C and 1kPa, place in a vacuum oven, dry at 60°C and 1kPa for 24 hours, and pulverize to obtain 840g of collagen powder. Afte...
example 2
[0020] Add 1500g of chicken adipose tissue into a large beaker, add 1000ml of neutral protease aqueous solution (containing 500,000 units of neutral protease), soak for 12 hours at 50°C, the chicken fat tissue will completely dissolve and separate layers, the upper layer is chicken oil, and the lower layer is collagen egg whites.
[0021] After the upper layer of chicken fat is collected and cooled, 1200g of chicken fat is obtained. After testing, each index reaches the national chicken fat standard.
[0022] The collagen liquid in the lower layer was collected, placed on a rotary evaporator, concentrated to 300ml at 60°C and 1kPa, and spray-dried directly to obtain 50g of collagen powder. After testing, the protein content has reached more than 90%, which can be sold as collagen.
example 3
[0024] Put 800g of otter subcutaneous fat into a beaker, add 100ml of papain aqueous solution (containing 100,000 units of papain), soak at 40°C for 8 hours, the subcutaneous fat tissue is completely dissolved and separated, the upper layer is otter oil, and the lower layer is collagen solution.
[0025] After the upper layer of otter oil is collected and cooled, 600 g of otter oil is obtained. After testing, each index reaches the otter oil standard stipulated by the state.
[0026] The collagen liquid in the lower layer was collected, placed on a rotary evaporator, concentrated to 180ml at 60°C and 1kPa, placed in a vacuum oven, dried at 60°C and 1kPa for 24 hours, and crushed to obtain 40g of collagen powder. After testing, the protein content has reached more than 90%, which can be sold as collagen.
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Abstract
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Application Information
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