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Method for preparing sesame-seed cake meal protein isolate

A technology for separating protein and sesame cake, which is applied in the field of protein separation technology

Inactive Publication Date: 2015-04-22
CHONGQING DOUHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to literature search, there are few reports on the research on protein isolate from sesame cake

Method used

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  • Method for preparing sesame-seed cake meal protein isolate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0017] Add 900 liters of pure water at 50°C to the reaction kettle, start stirring, add 100 kg of sesame low-temperature cake to prepare a 10% sesame cake solution, add a liquid with a concentration of 30% radon sodium oxide solution, adjust the pH to 8.0, and keep 15 minutes, and separated by a horizontal screw discharge centrifuge, and the liquid phase was recovered, and the obtained liquid phase was added with hydrochloric acid to adjust the pH value to 4.2, and centrifuged, and the obtained solid phase was added with 5 times of pure water, stirred evenly, and passed through Separation in a horizontal screw discharge centrifuge, recovering the solid phase, adding pure water, controlling the concentration at 15%, neutralizing the curd with sodium hydroxide, and neutralizing until the pH reaches 7.2 for flash sterilization. The temperature is 130°C and the flash negative Pressure -0.06MPa, then spray drying, the obtained sesame cake protein isolate product 47.1 kg. The obtain...

Embodiment 2

[0019] Add 920 liters of pure water at 50°C to the reaction kettle, start stirring, add 80 kg of sesame cake meal to prepare an 8% sesame cake meal solution, add a liquid with a concentration of 30% sodium hydroxide solution, adjust the pH to 9.5, and keep it at 15 minutes, and after horizontal

[0020] The spiral unloading centrifuge separates, recovers the liquid phase, adds hydrochloric acid to the obtained liquid phase to adjust the pH value to 4.4, and performs centrifugation, and adds 3 times of pure water to the obtained solid phase, stirs evenly, and passes through the horizontal spiral unloading centrifuge Separation by machine, recovery of solid phase, adding pure water, controlling the concentration at 15%, neutralizing the curd with sodium hydroxide, neutralizing to pH 7.0 for flash sterilization temperature 130°C, flash negative pressure -0.06MPa, and then Carry out spray drying, obtain 37.5 kilograms of isolated protein products of low-ash sesame cake. The obtai...

Embodiment 3

[0022] Add 950 liters of pure water at 50°C to the reaction kettle, start stirring, add 50 kg of sesame low-temperature cake to prepare a 5% sesame cake solution, add a liquid with a concentration of 30% sodium hydroxide solution, adjust the pH to 7.2, and keep 1 5 minutes, and separated by a horizontal screw discharge centrifuge, recovering the liquid phase, adding hydrochloric acid to the obtained liquid phase to adjust the pH value to 4.5, performing centrifugation, adding 4 times pure water to the obtained solid phase, and stirring evenly , separated by a horizontal screw discharge centrifuge, recovering the solid phase, adding pure water, controlling the concentration at 15%, neutralizing the curd with sodium hydroxide, and neutralizing until the pH reaches 7.0 for flash sterilization. The temperature is 130 ° C, flash Evaporate at a negative pressure of -0.06MPa, and then spray dry to obtain 23.1 kg of the isolated protein product of low ash sesame cake. The obtained pro...

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Abstract

The invention provides a method for preparing sesame-seed cake meal protein isolate. The sesame-seed cake meal protein isolate is prepared from low-temperature sesame-seed cake meal and 10-20 parts of pure water in an extraction manner. The method for preparing the sesame-seed cake meal protein isolate comprises the following steps: preparing 40-80-mesh low-temperature sesame-seed cake meal and pure water into a 5-10% solution, adding an alkali liquid, adjusting the pH value to be 7.0-10.0, performing centrifugal separation, recycling the liquid phase, adding hydrochloric acid into the obtained liquid phase to adjust the pH to be 4.0-4.6, adding pure water into the obtained solid phase for washing, uniformly stirring, adding the alkali liquid to neutralize until the pH value is 6.0-9.0, and performing spray drying, thereby obtaining sesame-seed cake meal protein isolate. The recycling rate of protein is greater than 90%, and the content of protein of a product is greater than 80%.

Description

technical field [0001] The invention relates to a protein separation process, in particular to a method for preparing sesame cake protein isolate. Background technique [0002] my country is a big country of sesame planting and consumption, accounting for 40% of the world's total. In addition to direct consumption of sesame, most of it is used for oil extraction, and the protein content of the by-product sesame cake after oil extraction can reach 40-50%, and the annual possession of sesame in our country reaches one million tons. Sesame cake is rich in sulfur-containing amino acids, especially methionine, which is significantly higher than other plant proteins, and has no anti-nutritional factors, and has comprehensive nutritional value. Its NPU (net protein utilization rate) is 56, and PER (protein efficacy ratio) is 1.8. Compared with other plant proteins, the amino acids contained in sesame protein are slightly lower than lysine, and other amino acids can be compared wit...

Claims

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Application Information

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IPC IPC(8): A23J1/14
Inventor 宁发子邓小玲
Owner CHONGQING DOUHAO BIOTECH
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