The method that thiourea compound is used for anti-tobacco mosaic virus activity

Abstract

The invention provides a method for resisting tobacco mosaic virus activity through a thiourea compound and relates to a plant virus resisting agent of a chemical compound. The method comprises the following steps that A, the thiourea compound is a compound provided with a structure shown by the following general formula (I), and please see the general formula in the specification; B, the tobacco mosaic virus activity resisting method of the thiourea compound comprises the subsidiary steps of tobacco mosaic virus refinement and concentration determination, preparation of a selected thiourea compound medicament solution, the in-vitro effect, the in-vivo protection effect, the in-vivo treatment effect and the in-vivo inactivation effect. The compound is used for preventing and curing the activity of diseases caused by tobacco mosaic viruses, and is better than chemical compounds and drugs in the prior art, low in production cost and capable of being widely applied to the agriculture.

Description

technical field [0001] The technical scheme of the present invention relates to the anti-plant virus agent of the chemical compound, specifically the method for the anti-tobacco mosaic virus activity of the thiourea compound. Background technique [0002] Plant viruses are widely distributed in nature. They cause serious damage to the growth of crops, and then cause great losses to modern agricultural production. Tobacco mosaic virus (TMV), as a pathogen, can cause harm to many crops. According to statistics, it can infect about 198 kinds of individuals of 22 kinds of monocotyledonous plants, and can cause losses ranging from about 5% to 90% (Hari, V.; Das, P. Ultra microscopic detection of plant viruses and their gene products. In plant disease virus control. Hadidi, A.; Khetarpal, R.K.; Koganezawa, H., Eds.; APS Press: St.Paul, MN, 1998:417-427.) So far, there is no chemical pesticide that can Thoroughly protect crops from TMV. Likewise, there is no single drug that can ...

AI Technical Summary

Problems solved by technology

[0003] In the prior art, ribavirin (Ribavirin), as a relatively successful plant virus inhibitor, is widely used to prevent and treat diseases caused by tobacco mosaic virus. The inhibition rate in the in vitro activity, in vivo passivation, in vivo treatment and in vivo protection of leaf virus is less than 50%; when the dosage of Ningnanmycin produced from microbial fermentation is 500 μg / mL, the therapeutic effect on tobacco mosaic virus Only about 54%; Literature [Mei-hang Chen, Zhuo Chen, Bao-an Song, *Pinaki S. Bhadury, Song Yang, Xun-jianCai, De-yu Hu, Wei Xue, Song Zeng. Synthesis and antiviral activities of chiralthiourea derivatives containing anα-aminophosphonate moiety.J.Agric.FoodChem.,2009,57(4),1383-1388] reported chiral thiourea derivatives containing α-phosphonate and their preparation methods and uses, some of which When the concentration is 500 μg / mL, the therapeutic effect on tobacco mosaic virus (inhibition rate is 54.8%) is lower than that of the reference drug Ningnanmycin (inhibition rate is 56.2%) under the same test conditions; CN 200910102561.1 discloses a drug containing Chiral dithiourea derivatives of phosphonate esters and their preparation methods and uses. Under the same test conditions, some of the compounds have low therapeutic effects on tobacco mosaic virus (inhibition rate: 50.8%) when the concentration is 500 μg / mL The reference drug Ningnanmycin (inhibition rate was 55.3%); literature [Yan Zhikun, Cai Xuejian, Yang Xuan, Song Baoan, Chen Zhuo, Pinaki S. Bhadury, Hu Deyu, Jin Linhong, Xue Wei, Lu Ping. Synthesis and antiviral activities of chiralthiourea, Chinese Journal of Chemistry, 2009,27,593-601.)] reported that when the dosage of indenyl-containing chiral thiourea compound was 500mg / L, the inhibition rates of in vivo protection, in vivo inactivation and in vivo treatment were 57.0%, respectively, 96.4% and 55.0%, under the same test conditions, the commercial variety Ningnanmycin was 55.9%, 96.5%, and 53.9%, although the protective and therapeutic effects were slightly higher than that of the control drug Ningnanmycin, the passivation effect and Ningnanmycin is quite, but the synthetic needs raw material of this chiral thiourea compound (S)-1-(1-indenyl)-3-phenylthiourea is (S)-1-aminoindenane (selling price For about 580 yuan / gram) and phenyl isothiocyanate, the price is expensive; in a word in this technical field The chiral thiourea compound reagent used is generally expensive; CN201410344605.2 discloses a method in which 3-aryl-5-methylbutyrolactone compound is used as an anti-plant virus agent, and its existing defect is also harmful to tobacco leaves. The inhibition rate in the in vitro activity, in vivo passivation, in vivo treatment and in vivo protection of the virus is still low (the compound disclosed in CN201410344605.2, the compound 3-(4-fluorophenyl)- The in vivo treatment and in vivo protection inhibition rates of 5-methyl-γ-butyrolactone were 57.1% and 60.0%, respectively, and the in vivo treatment and in vivo protection inhibition rates of the commercial variety Ningnanmycin were 54% under the same test conditions and 64%)

Images