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Antibacterial peptide preparation, and preparation method and application thereof

An antibacterial peptide and antibacterial agent technology, applied in the field of antibacterial peptide preparations and their preparation, can solve the problems of low encapsulation rate, high cost, poor stability and the like, and achieve the effects of high encapsulation rate, reduced dissolution loss and good stability

Active Publication Date: 2015-04-08
GUANGDONG HINAPHARM PHARMA CO LTD
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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide an antimicrobial peptide preparation and its preparation method and its application, aiming to solve the problems of low encapsulation efficiency, poor stability and high cost in the current preparation technology of antimicrobial peptide preparations

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  • Antibacterial peptide preparation, and preparation method and application thereof
  • Antibacterial peptide preparation, and preparation method and application thereof
  • Antibacterial peptide preparation, and preparation method and application thereof

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Embodiment Construction

[0025] The present invention provides an antimicrobial peptide preparation and its preparation method and application. In order to make the purpose, technical solution and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific examples described here are only used to explain the present invention, and are not intended to limit the present invention. The present invention provides a preparation method of antimicrobial peptide preparations, wherein the antimicrobial peptide producing bacteria used to produce antimicrobial peptides are Pichia pastoris recombinant strain GS115 / pPicZaMF57-PLE118, the construction process of the recombinant strain is as follows:

[0026] Recombinant plasmid construction and screening transformation: According to the codon preference of exogenous yeast protein expression, the plectasin nucleotide sequence was optimized and artificially synthesized....

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Abstract

The invention discloses an antibacterial peptide preparation, and a preparation method and application thereof. The preparation method comprises the following steps: A, concentrating a thallus fermentation liquid of an antibacterial-peptide producing strain, adding beta-cyclodextrin, dextrin and Arabic gum into the concentrate, and emulsifying for 5-10 min; B, adding the mixed solution into a high-speed centrifugation spray dryer for spray drying, so as to prepare a semi-finished product particle; and C, utilizing a dressing liquid consisting of stearic acid, glyceryl monostearate and glycerol to do dressing processing on the semi-finished product particle in a fluidized-bed dressing machine, so as to prepare the finished product. The preparation method is simple in technology and low in cost, and is suitable for large-batch programmed production. The antibacterial peptide preparation prepared through the method is high in encapsulation rate and good in stability, thereby improving the stability and the shelf life of the antibacterial peptide during forage processing and storage, reducing dissolution loss of the antibacterial peptide in water during forage feeding, improving the utilization rate of the antibacterial peptide and reducing pollution to water.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to an antimicrobial peptide preparation and its preparation method and application. Background technique [0002] Antimicrobial peptides (AMPs) are an important part of animal innate immunity, the first barrier for animals and plants to defend against microbial invasion, and have broad-spectrum antibacterial effects. It has a killing effect, and the effective concentration is extremely low, and some can completely inhibit the growth of bacteria even at the nmol concentration. Compared with traditional antibiotics, the biggest feature of antimicrobial peptides is that their bactericidal mechanism is that the hydrophobic terminal helix is ​​directly inserted into the bacterial cell membrane to form pores, and the bacterial contents are leaked out to die. The effect is rapid and effective against drug-resistant bacteria. Not easy to develop drug resistance. The experimenta...

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Application Information

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IPC IPC(8): A61K38/16A61K9/16A61K47/40A61P31/04A23K1/16
Inventor 逯佩凤周玉岩代珍青闫凯叶滔
Owner GUANGDONG HINAPHARM PHARMA CO LTD
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