Method for rapidly identifying resistance of apple branches and trunks to physalospora piricola nose

Abstract

The invention relates to a physalospora piricola nose resistance identification method, in particular to a method for rapidly identifying resistance of apple branches and trunks to the physalospora piricola nose. The physalospora piricola nose is inoculated to a PDA culture medium to be cultured so that a physalospora piricola nose culture medium bacterial colony can be obtained; pseudo identified varieties or rootstock young sprout leaves without plant diseases or insect pests at the top end, the front faces of the leaves are stabbed by an inoculating needle, and then the physalospora piricola nose is inoculated; after the bacteria-bearing leaves are cultured for six days, the diameters of disease spots of the leaves are measured according to a crossing method, the disease spot area of the leaves are worked out and compared with the disease spot areas of known resistance grade materials, and the grade of resistance of varieties to be identified or rootstocks to the branch and trunk physalospora piricola nose is determined. According to the method for rapidly identifying the resistance of apple branches and trunks to the physalospora piricola nose, leaves are inoculated, infection conditions suitable for the physalospora piricola nose are created, physalospora piricola nose resistance identification is performed on the infection result, few identification materials are needed, identification time is short, repeatability is high, and the identification method is simple, easy to master and popularize, and low in cost.

Description

technical field [0001] The invention relates to a method for identifying resistance to ringworm, in particular to a method for quickly identifying the resistance of apple branches to ringworm. Background technique [0002] At present, the methods for identifying and evaluating the resistance of apple branches to ring spot mostly adopt the method of branch inoculation in the field, investigation of the disease level and calculation of the disease index. It is greatly affected by natural environmental conditions, resulting in long-term resistance identification, cumbersome identification methods, poor repeatability of identification results, and easy spread of field diseases. Contents of the invention [0003] In view of the above-mentioned technical problems, the present invention provides a method for identification of ring spot resistance, which makes the identification of apple varieties or rootstocks resistant to ring spot simple, takes a short time, and has good repeat...

AI Technical Summary

Problems solved by technology

[0002] At present, the methods for identifying and evaluating the resistance of apple branches to ring spot mostly adopt the method of branch inoculation in the field, investigation of the disease level and calculation of the disease index. Affected by natural environmental conditions, the resistance identification takes a long time, the identification method is cumbersome, the identification effect is poor, and it is easy to cause the spread of field diseases

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