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Mesoporous nano silicon sphere composite targeted drug delivery system and its preparation method and application

A technology of mesoporous nano-silica spheres and targeted drug delivery, which is applied in the field of medicine, can solve the problems of multi-drug resistance of cells, strong toxic and side effects of human health, etc., and avoid the adverse effect of multi-drug resistance and strong cytotoxicity , High therapeutic index effect

Inactive Publication Date: 2017-03-08
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Based on the mechanism of multidrug resistance of tumor cells, a large number of repeated treatments of cancer with a single drug will lead to the generation of multidrug resistance in cells. Higher doses of the drug, which lead to more toxic side effects on human health

Method used

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  • Mesoporous nano silicon sphere composite targeted drug delivery system and its preparation method and application
  • Mesoporous nano silicon sphere composite targeted drug delivery system and its preparation method and application
  • Mesoporous nano silicon sphere composite targeted drug delivery system and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Preparation of FITC-MSNs-HA-RGD-DOX--PTX Components

[0039] (1) Preparation of amino-functionalized drug-loaded mesoporous silica microspheres

[0040] Add 0.4270g of anticancer drugs PTX and 1.8232g of CTAB into 500mL of distilled water, under sealed conditions, stir vigorously at 80°C for several minutes to obtain a clear solution, add NH 4 F1.4816g, immediately add TEOS8.4592g at a rate of 0.45mL / min, dropwise for 20 minutes. After reacting for 1 hour, a translucent colloidal state solution was obtained, centrifuged at -4°C for 15 minutes at 13000 rpm, discarded the supernatant, washed three times with a mixed solution of ethanol and concentrated hydrochloric acid (150 mL: 2 mL) and distilled water, and freeze-dried for 24 hours to obtain a white Powder (PTX-MSNs), FTIR, SEM, TEM, XRD and other characterizations.

[0041] Take the PTX-MSNs prepared in the previous step, 30mL of anhydrous toluene, in N 2 Stir and mix evenly under protection, slowly raise the te...

Embodiment 2

[0060]1. Preparation of FITC-MSNs-HA-RGD-DOX--PTX Components

[0061] (1) Preparation of amino-functionalized drug-loaded mesoporous silica microspheres

[0062] Add 0.2135g of anticancer drugs PTX0.2135g and CTAB0.6116g into 500mL of distilled water, under sealed conditions, stir vigorously at 80°C for several minutes to obtain a clear solution, add NH 4 F0.7208g, immediately add TEOS4.2296g at a rate of 0.45mL / min, dropwise for 20 minutes. After reacting for 1 hour, a translucent colloidal state solution was obtained, centrifuged at -4°C for 15 minutes at 13000 rpm, discarded the supernatant, washed three times with a mixed solution of ethanol and concentrated hydrochloric acid (150 mL: 2 mL) and distilled water, and freeze-dried for 24 hours to obtain a white Powder (PTX-MSNs), FTIR, SEM, TEM, XRD and other characterizations.

[0063] Take the PTX-MSNs prepared in the previous step, 30mL of anhydrous toluene, in N 2 Stir and mix evenly under protection, slowly raise the ...

Embodiment 3

[0082] 1. Preparation of FITC-MSNs-HA-RGD-DOX--PTX Components

[0083] (1) Preparation of amino-functionalized drug-loaded mesoporous silica microspheres

[0084] Add 0.8540 g of anticancer drugs PTX and 1.8232 g of CTAB into 500 mL of distilled water, under sealed conditions, stir vigorously at 80 ° C for several minutes to obtain a clear solution, add NH 4 F2.9632g, immediately add TEOS8.4592g at a rate of 0.45mL / min, dropwise for 20 minutes. After reacting for 1 hour, a translucent colloidal state solution was obtained, centrifuged at -4°C for 15 minutes at 13000 rpm, discarded the supernatant, washed three times with a mixed solution of ethanol and concentrated hydrochloric acid (150 mL: 2 mL) and distilled water, and freeze-dried for 24 hours to obtain a white Powder (PTX-MSNs), FTIR, SEM, TEM, XRD and other characterizations.

[0085] Take the PTX-MSNs prepared in the previous step, 30mL of anhydrous toluene, in N 2 Stir and mix evenly under protection, slowly raise t...

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Abstract

The invention relates to a mesoporous nano silicon ball compound targeting drug delivery system as well as a preparation method and application thereof. The preparation method of the mesoporous nano silicon ball compound targeting drug delivery system comprises the following steps: 1) preparing amino-functionalized drug loading mesoporous silicon dioxide microspheres; 2) preparing hyaluronic acid-hydrosulphonyl polypeptide-adriamycin (HA-RGD-DOX); 3) preparing mesoporous microsphere-hyaluronic acid-hydrosulphonyl polypeptide-adriamycin--paclitaxel (MSNs-HA-RGD-DOX_PTX); and 4) preparing fluorescent marker modified mesoporous microsphere-hyaluronic acid-hydrosulphonyl polypeptide-adriamycin--paclitaxel compound (MSNs-HA-RGD-DOX-PTX). The mesoporous nano silicon ball compound targeting drug delivery system has the beneficial effects that firstly multi-targeting synergistic drug delivery is realized, multiple tumour cells and tissues can be killed, and reversal drug resistance is good; secondly, blood stability is excellent; thirdly, invisibility, drug release degree and controlled release properties are good; fourthly, in vivo tracing function is good; and fifthly, the mesoporous nano silicon ball compound targeting drug delivery system has good general applicability.

Description

technical field [0001] The invention relates to the field of medicines, in particular to a mesoporous nano silicon sphere complex targeted drug delivery system, a preparation method of the system and an application of the system. Background technique [0002] In recent years, with the increasing morbidity and mortality of cancer, cancer has become one of the diseases with the highest fatality rate. At present, the treatment of malignant tumors is mainly combined with surgery and chemotherapy, of which chemotherapy is a must. means. Drug resistance is a major problem in cancer treatment, and it is also a common problem with current chemotherapy drugs. Studies have shown that more than 90% of malignant tumor patients die of multi-drug resistance (MDR). [0003] Based on the mechanism of multidrug resistance of tumor cells, a large number of repeated treatments for cancer with a single drug will lead to the generation of multidrug resistance in cells. Higher doses of drugs, w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K9/19A61K31/704A61K49/00A61K47/04A61P35/00A61K31/337
Inventor 徐海星汪志辉许沛虎黄志军徐腾飞聂壮赵亚琼牛小倩张凌溪
Owner WUHAN UNIV OF TECH
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