Protein chip for diagnosing porcine virus diarrhea
A porcine viral diarrhea and protein chip technology is applied in the field of bioinformatics, which can solve problems such as economic losses
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[0012] 1. Selection of polypeptide position and length
[0013] Using bioinformatics methods to analyze the protein sequences with characteristic epitopes in the extracellular parts of the three viral membrane proteins (M protein and S protein), and then analyze the position and homology of these sequences on the protein and membrane, according to According to the corresponding characteristics, a certain length is reasonably selected and sent to Shanghai Bioengineering Co., Ltd. for synthesis.
[0014] 2. Immunization of hens
[0015] The synthesized polypeptide was coupled with BSA, fully emulsified with Freund's complete adjuvant for the first time, multi-point subcutaneous injection to immunize hens, and incomplete adjuvant was used for the second and subsequent immunizations, with a time interval of half a month , After the fourth immunization is completed, the antibody is extracted, the titer is tested, and the chickens whose titer, sensitivity and specificity do not mee...
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