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D dimer latex-enhanced immunoturbidimetric assay kit utilizing surface functional group

A surface functional group and detection kit technology, applied in the field of D-dimer latex-enhanced immune turbidimetric detection kits, can solve problems such as time-consuming, and achieve the effects of increasing sensitivity, simplifying operation methods, and ensuring cross-linking effect.

Active Publication Date: 2015-03-25
ZYBIO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, colloidal gold method is widely used clinically. ELISA operation is strict and time-consuming. As a qualitative or semi-quantitative method, colloidal gold is slightly insufficient for quantification.

Method used

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  • D dimer latex-enhanced immunoturbidimetric assay kit utilizing surface functional group
  • D dimer latex-enhanced immunoturbidimetric assay kit utilizing surface functional group
  • D dimer latex-enhanced immunoturbidimetric assay kit utilizing surface functional group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A D dimer latex enhanced immune turbidimetric detection kit, comprising the following preparation steps:

[0040] (1) Preparation of reagent R1:

[0041] Weigh 1.9g stachyose, 0.5g alum, 1.9g sodium fructose diphosphate, 0.3g sodium hexametaphosphate, 1.8g glycine, 27g NaCl, 35g PEG8000, 0.5g sodium azide, 10g bovine serum albumin and 1.42g Dissolve EDTA in 0.8L of distilled water, adjust the pH to 7.4, and dissolve 1L to obtain reagent R1.

[0042] (2) Preparation of reagent R2:

[0043] The above-mentioned washed polystyrene latex microspheres were diluted to a mass concentration of 1% with PBS buffer solution having a pH of 7.4 and a concentration of 50 mmol / L. Add 0.01g of EDC to 1L of the above latex dilution, stir and react at room temperature for 60min, centrifuge after the reaction and wash with PBS buffer to remove unreacted EDC, then add D-dimer antibody, stir and react at 37°C for 4h , add the stop solution 4h to terminate the reaction, centrifuge and wash...

Embodiment 2

[0045] A D dimer latex enhanced immune turbidimetric detection kit, comprising the following preparation steps:

[0046] (1) Preparation of reagent R1:

[0047] Weigh 1.9g stachyose, 0.5g alum, 1.9g sodium fructose diphosphate, 0.3g sodium hexametaphosphate, 1.8g glycine, 30g NaCl, 50g PEG8000, 0.5g sodium azide, 50g bovine serum albumin and 7.1g Dissolve EDTA in 0.8L of distilled water, adjust the pH to 7.4, and dissolve 1L to obtain reagent R1.

[0048] (2) Preparation of reagent R2:

[0049] Dilute the polystyrene latex microspheres to a mass concentration of 3% with MES buffer solution with a pH of 6.5 and a concentration of 100 mmol / L. Add 0.01g of EDC to 1L of the above latex dilution, stir and react at room temperature for 30min, centrifuge after the reaction and wash with PBS buffer to remove unreacted EDC, then add D-dimer antibody, stir and react at room temperature for 8h, Add stop solution for 4 hours to terminate the reaction, centrifuge and wash the obtained r...

Embodiment 3

[0051] A D dimer latex enhanced immune turbidimetric detection kit, comprising the following preparation steps:

[0052] (1) Preparation of reagent R1:

[0053] Weigh 1.9g stachyose, 0.5g alum, 1.9g sodium fructose diphosphate, 0.3g sodium hexametaphosphate, 1.8g glycine, 15g NaCl, 24g PEG8000, 0.5g sodium azide, 10g bovine serum albumin and 7.1g Dissolve EDTA in 0.8L of distilled water, adjust the pH to 7.4, and dissolve 1L to obtain reagent R1.

[0054] (2) Preparation of reagent R2:

[0055] Dilute the polystyrene latex microspheres to a mass concentration of 2% with MES buffer at a pH of 6.0 and a concentration of 100 mmol / L. Add 0.02g of EDC to 1L of the above latex dilution, stir and react at room temperature for 30min, centrifuge after the reaction and wash with PBS buffer to remove unreacted EDC, then add D-dimer antibody, stir and react at room temperature for 8h, Add stop solution for 4 hours to terminate the reaction, centrifuge and wash the obtained reaction sol...

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Abstract

The invention relates to an in vitro diagnostic kit, and in particular relates to a D dimer latex-enhanced immunoturbidimetric assay kit utilizing a surface functional group. The D dimer latex-enhanced immunoturbidimetric assay kit utilizing the surface functional group consists of two components, namely a reagent R1 and a reagent R2, wherein the reagent R1 mainly consists of a buffer solution 1, a stabilizer 1, a preservative 1, a gelling agent and a protective agent 1; the reagent R2 mainly consists of a buffer solution 2, two polystyrene latex microspheres cross-linked to different D dimer monoclonal antibodies, a stabilizer 2, a protective agent 2 and a preservative 2; the polystyrene latex microspheres are connected to the D dimer antibodies in a manner of covalent cross-linking or physical adsorption; and the surface functional group of the polystyrene latex microsphere in the reagent R2 is an amino group, a carboxyl group, hydrazide, an aldehyde group or an epoxy group. Compared with the prior art, by virtue of the latex-enhanced immunoturbidimetric assay, the kit can be used for detecting by wavelength of 400-800nm, so that the kit is more convenient to detect and is easy in clinical application.

Description

technical field [0001] The invention relates to an in vitro diagnostic kit, in particular to a D-dimer latex-enhanced immunoturbidimetric detection kit utilizing surface functional groups. Background technique [0002] The fibrinolytic system is the most important anticoagulant system in the human body and consists of four main components: plasminogen, plasminogen activator, plasmin, and plasmin inhibitor. When a fibrin clot is formed, in the presence of plasminogen activators, plasminogen is activated and converted into plasmin, the process of fibrinolysis begins, and plasmin degrades the fibrin clot to form various soluble fragments, Formation of fibrin product (FDP), FDP is composed of X-oligomer, D-dimer, middle fragment and fragment E (Fragment E). Among them, both X-oligomer and D-mer contain D-dimer unit. [0003] The fibrinolytic system of the human body plays an important role in maintaining the normal permeability of blood vessel walls, maintaining blood flow and...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 高昂
Owner ZYBIO INC
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