A method for long-term preservation of Cordyceps militaris strains

Abstract

The invention relates to the technical field of preservation of strains and in particular relates to a method for preserving a cordyceps militaris strain for a long time. The method comprises the following steps: A. culturing the strain, namely inoculating a healthy cordyceps militaris strain to a sterilized PDA inclined medium under aseptic condition, carrying out dark culture, and carrying out light culture after hyphae overgrows 40%-60% of the slope; B. dewatering the culture medium, namely putting a test tube filled with culture into a drying vessel, then putting the drying vessel in a refrigerator, and taking out the test tube after the inclined medium is dewatered until 20%-30% of volume is left; and C. preserving the strain, namely slowly injecting sterilized paraffin oil into the test tube till the paraffin oil immerses the culture by over 1cm, shaking and removing bubbles on the surface of the culture, plugging sterilized and degreased cotton, sealing by using sterilized paraffin, covering a sterilized pp film, vertically arranging the test tube in the refrigerator at 0 DEG C for preserving. By virtue of the method, the physiological activity of the strain can be well inhibited; the strain can be preserved for more than 3 years; the preserved strain also has good activity.

Description

technical field [0001] The invention relates to the technical field of strain preservation, in particular to a method for long-term preservation of strains of Cordyceps militaris. Background technique [0002] The general steps of the existing paraffin preservation method are as follows: sterilize the liquid paraffin, put it in a thermostat at 37° C., and evaporate the water vapor for subsequent use. Then culture the purified bacteria to be stored in the most suitable slant medium to obtain robust bacteria, absorb sterilized liquid paraffin with a sterile pipette, inject it into the grown slant medium, and use an amount of 1cm higher than the inclined surface shall prevail, and the test tube shall be erected, labeled, and stored at 4°C. This method is simple to make and does not require special equipment. The strain can be preserved for about one year without frequent transplantation. The disadvantage is that it needs to be placed upright when preserved. [0003] However, ...

AI Technical Summary

Problems solved by technology

This method is simple to make and does not require special equipment. The bacteria can be stored for about 1 year without frequent transplantation. The disadvantage is that it needs to be placed upright during storage.

[0003] However, for some strains with tenacious vitality, such as Cordyceps militaris strains, the existing paraffin preservation method is not applicable. Therefore, it is necessary to develop a long-term preservation method that can better inhibit the physiological activities of the strains