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Efficient probiotics microcapsule as well as preparation method and application thereof

A microcapsule, high-efficiency technology, applied in applications, animal feed, animal feed, etc., can solve problems such as the influence of probiotic survival rate, and achieve the effects of improving tolerance, increasing the number of viable bacteria, and reducing losses

Inactive Publication Date: 2015-03-25
NANJING YOUFAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different wrapping materials and different processing techniques have a great impact on the survival rate of probiotics

Method used

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  • Efficient probiotics microcapsule as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of Saccharomyces cerevisiae microcapsules.

[0019] Saccharomyces cerevisiae seed solution was inoculated on the culture medium at an inoculation amount of 3wt%, cultivated at 37°C and 120r / min for 16h, centrifuged at 6000r / min for 5 minutes, and added 2wt% of porous starch and 10% of Skimmed milk powder, mixed with a high-speed homogenizer to obtain the core material; weigh sodium alginate and add it to distilled water to prepare a 3wt% sodium alginate aqueous solution, autoclave to obtain the first layer of wall material, and add the core material after cooling to room temperature. Mix well, the volume ratio of core material and wall material is 1:2, pass through Equl microcapsule generator, and add 8g / l CaCl dropwise 2 , solidified for 40 min, and collected the microcapsules by filtration. The microcapsules were suspended in distilled water at a concentration of 10 wt%. It was fluidized spray embedded with 5 wt% gelatin. The parameters are th...

Embodiment 2

[0020] Example 2 Preparation of Lactobacillus acidophilus microcapsules.

[0021] Inoculate the seed solution of Lactobacillus acidophilus in the culture medium at an inoculum amount of 3wt%, cultivate at 37°C and 120r / min for 14h, centrifuge at 8000r / min for 5 minutes, and add porous starch accounting for 2% of the total weight of Lactobacillus acidophilus cell precipitation Mix with 10wt% skimmed milk powder with a high-speed homogenizer to obtain the core material; add 3wt% sodium alginate to distilled water and mix, autoclave to obtain the first layer of wall material, cool to room temperature, add the core material and mix well , the volume ratio of the core material to the wall material is 1:2, through the Equl microcapsule generator, and 8g / l CaCl is added dropwise 2 , solidified for 40 min, and collected the microcapsules by filtration. Suspend the microcapsules in distilled water at a concentration of 10 wt%. It was fluidized spray embedded with 5 wt% gelatin. The ...

Embodiment 3

[0022] Example 3 Preparation of Enterococcus faecium microcapsules.

[0023] Inoculate the seed solution of Enterococcus faecium on the culture medium at an inoculum amount of 3wt%, cultivate at 37°C and 120r / min for 14h, centrifuge at 8000r / min for 5 minutes, add porous starch and 10wt% of the total weight of Enterococcus faecium thallus % skimmed milk powder, mixed with a high-speed homogenizer to obtain the core material; weigh sodium alginate and add it to distilled water to prepare a 3wt% sodium alginate aqueous solution, and autoclave to obtain the first layer of wall material, and add the core material after cooling to room temperature Mix the material well, the volume ratio of the core material and the wall material is 1:2, pass through the Equl microcapsule generator, and add 8g / l CaCl dropwise 2 , solidified for 40 min, and collected the microcapsules by filtration. The microcapsules were suspended in distilled water at a concentration of 10 wt%. It was fluidized s...

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Abstract

The invention discloses an efficient probiotics microcapsule which comprises a core material and a wall material, wherein the core material comprises 90-95wt% of probiotics and 5-10wt% of a protector; the wall material is of a double-layer structure, wherein the inner layer is made from sodium alginate and the outer layer is made from whey protein or gelatin. The invention also discloses a preparation method and application of the efficient probiotics microcapsule. The microcapsule is coated by double layers of the sodium alginate and the gelatin or whey protein, so that the acid resistance, cholate resistance and storage resistance of the probiotics are improved. The microcapsule disclosed by the invention is added into the feed, so that the host intestinal microenvironment equilibrium can be regulated, the harmful bacteria can be restrained and the disease resistance of the host can be improved.

Description

technical field [0001] The invention belongs to the field of feed additives and relates to a probiotic, in particular to a high-efficiency microcapsule of a probiotic and a preparation method and application thereof. Background technique [0002] As a feed additive, probiotics can adjust the microbial balance in the gastrointestinal tract of livestock and improve animal production performance. Probiotics are live bacteria that must reach the gut of a livestock animal to be effective. However, high temperature and mechanical force in feed processing, long-term storage and transportation, gastric acid and bile in gastrointestinal fluid will kill probiotics, and probiotics that can enter the intestinal tract alive and play a role through ordinary feeding methods Small number. [0003] Microcapsule technology is a kind of microcapsule technology with semi-permeable or sealed capsule membrane formed by encapsulating solid, liquid or gas core substances with natural or polymer e...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/00
Inventor 张俊生
Owner NANJING YOUFAN BIOTECH
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