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Method for determining content of trace elements in DunalieUa salina

A technology of Dunaliella salina, medium and trace elements, applied in the direction of color/spectral characteristic measurement, etc., which can solve the problems of poor practicability, troublesome operation, and high requirements for testers

Active Publication Date: 2015-03-11
沂水盛源食品有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Their invention has accurate and stable measurement results, good reproducibility, safety and pollution-free, but the shortcomings are also obvious, such as troublesome operation, strong technicality, high requirements for testers, expensive equipment, large investment in instruments, and poor practicability

Method used

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  • Method for determining content of trace elements in DunalieUa salina

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Embodiment Construction

[0023] Determination method of β-carotene content

[0024] 1) In step 1, when Dunaliella salina is cultured to the 13th day, the cell density of the algae can reach 250×10 4 -400×10 4 cell / per milliliter, pipette 100 milliliters of algae liquid, and pack in a 250 milliliter Erlenmeyer flask;

[0025] 2) Add 15 grams of NaCl in step 2, add 120 ml of 80% ethylene glycol (mixed by ethylene glycol and distilled water in a volume ratio of 80:20), seal with plastic wrap, tie the rubber band tightly, and shake evenly on a shaker for 20 Minutes, full extraction;

[0026] 3) In step 3, the liquid is divided into supernatant and turbidity at this time, and the supernatant is pipetted with a 20 ml pipette, added to a 250 ml volumetric flask, and the volume is adjusted to 250 ml with 80% ethylene glycol;

[0027] 4) Step 4 is transferred to a 250 ml round-bottomed flask, shaken evenly on a shaker for 60 minutes, and measures the absorbance value OD at a wavelength of 425 nm with a 721 ...

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Abstract

Belonging to the field of aquaculture, the invention discloses a method for determining the content of beta-carotene and chlorophyll a in Dunaliella salina. The traditional beta-carotene content determination method consists of: diluting a beta-carotene standard substance into a serial concentration standard substance, and subjecting an obtained sample solution to liquid chromatographic analysis, thus acquiring the content of beta-carotene in the sample. The method has the advantages of accurate result and low test cost, but has the disadvantages of long time, expensive equipment, and tedious operation. Determination of chlorophyll in seaweed traditionally adopts a spectrophotometer method, which is characterized by troublesome operation, strong technicality and expensive equipment. The extraction method disclosed by the invention for determining the content of beta-carotene and chlorophyll a in Dunaliella salina has the characteristics of simple operation, short time, accurate result and low cost, and can finish determination of a plurality of samples at one time.

Description

technical field [0001] The invention belongs to the field of aquaculture, in particular to a method for determining the content of beta-carotene and chlorophyll a in Dunaliella salina. Background technique [0002] Dunaliella salina is a diflagellated single-celled green alga widely distributed in inland salt lakes. It can tolerate high salinity (20-300‰), and the individual is large (16-24μm×10-13μm), because it can synthesize a large amount of β-carotene (up to 14% dry weight) has been widely valued. β-carotene is the precursor of vitamin A in the human body. It can be used to prevent and treat degenerative diseases such as cancer, cardiovascular disease and senile dementia. It can also be used as a high-grade natural pigment for food processing. Clinical trials have shown that salinadin extracted from Dunaliella salina has a good curative effect on regulating blood pressure, lowering blood fat, lowering blood sugar, diabetes, alcoholic liver, fatty liver, gastric ulcer, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 王培磊
Owner 沂水盛源食品有限公司
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