Online concentration device of post-column component of chromatographic column and control process

Abstract

The invention discloses an online concentration device of a post-column component of a chromatographic column and a control process. An inner cavity of a concentration pot is divided into a plurality of abreast concentration cavities, and a condensation pot is fixedly arranged at the tail end of the concentration pot; an inlet of a detector flow cell is communicated with the chromatographic column, and an outlet of the detector flow cell is communicated to the upper ends of all the concentration cavities of the concentration pot; solenoid valves are arranged on branches of all the concentration cavities, and discharge openings are respectively formed in the lower ends of all the concentration cavities; a heat medium pump is communicated to the lower ends of all the concentration cavities, a negative pressure pump is communicated to the upper ends of all the concentration cavities and the cavity of condensation pot, and a cold medium pump is communicated to the lower end of the inner cavity of the condensation pot; an exhausting opening of the condensation pot is communicated with a regenerative solvent tank. Through the use of the device, the online concentration of post-chromatographic column liquid and the online recovery of chemical solvent can be realized, so that not only is the concentration efficiency improved, but also the device is free from the limitation of the stock of the post-chromatographic column liquid in concentration, the whole process is performed in a sealed environment, and the device is suitable for extracting a concentrated solution of an object easy to oxidize and deteriorate; generated impurities are recycled, and pollution to the environment is avoided.

Description

technical field [0001] The invention relates to the relevant technical field of pharmaceutical equipment, in particular to the application equipment for the separation, concentration and recovery of liquid components in the natural Chinese herbal medicine and biopharmaceutical industries, in particular to an on-line concentration device for post-column components of a chromatographic column. Background technique [0002] The chromatographic purification method based on liquid chromatography technology has been applied in the chemical industry and the pharmaceutical industry in recent years to achieve relatively pure target substances separated from a large number of mixture solvents. Because this method has advanced testing equipment and electronic computer, it is very reliable and is more and more popular among professionals. [0003] At present, in the process of purification and separation using chromatographic columns in production, a large amount of chemical solvents ar...

AI Technical Summary

Problems solved by technology

The characteristics of the two are: the distillation kettle has a large processing capacity at one time, has losses, is prone to cross-contamination, and has high equipment costs

The rotary evaporator has too little treatment capacity at one time, which is time-consuming, and the post-column liquid containing impurities is often discarded, polluting the environment

At the same time, the two treatment methods are not suitable for targets that are easily oxidized and deteriorated.

Images