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Chromogenic medium used for detecting escherichia coli O157:H7

A chromogenic culture medium, O157 technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of no Escherichia coli, false positive of chromogenic culture, etc.

Inactive Publication Date: 2015-03-04
SOOCHOW KH BIO SCI & TECH CO LTD KHB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The study found that there are strains in Escherichia coli that do not ferment sorbitol and are positive for β-glucuronidase, but the EHECO157:H7 serum does not agglutinate, causing false positives for the chromogenic culture of EHEC O157:H7
Currently, there is no chromogenic medium for the detection of E. coli O157:H7

Method used

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  • Chromogenic medium used for detecting escherichia coli O157:H7
  • Chromogenic medium used for detecting escherichia coli O157:H7

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 10g of agar, 10g of peptone, 5g of yeast extract, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.1 g, 5-bromo-4-chloro-3-indole-β-galactose 0.1g, 4-methylfanone-β-D-glucuronide 0.1g, isopropyl-β-D-thio Galactopyranoside 0.1g, ox bile salt 1.5g, potassium tellurite 2mg, cefixime 0.04mg.

Embodiment 2

[0041] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 10g of agar, 10g of peptone, 5g of yeast extract, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.1 g, o-nitrophenyl-β-D-galactoside 2g, 4-methylfanone-β-D-glucuronide 0.1g, isopropyl-β-D-thiogalactopyranoside 0.1 g, ox bile salt 1.5g, potassium tellurite 2mg, cefixime 0.04mg.

Embodiment 3

[0042] Embodiment 3 specificity experiment

[0043]E.coli O157:H7NCTC12900, E.coli O157:H7882364, E.coli O157:H7ATCC43895, E.coli O157:H7ATCC43888, E.coli ATCC8739, E.coli CMCC(B)44102, E.coli CMCC(B) 44103, P. vulgaris CMCC (B) 49027 and other 8 kinds of standard bacterial strains were respectively made into standard bacterial suspensions of appropriate concentration (concentration is 108 ~ 109 cfu / mL), respectively streaked and inoculated to the chromogenic medium prepared in Example 1. cultured at 37°C for 18-24 hours. The specificity test results are shown in Table 1.

[0044] Table 1 The specific color test results of E.coli O157:H7 chromogenic medium

[0045]

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PUM

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Abstract

The invention discloses a chromogenic medium used for detecting escherichia coli O157:H7 and other intestinal floras. The medium contains agar, peptone, a yeast extract, sodium chloride, sorbitol, inositol, neutral red, a beta-galactosidase chromogenic substrate, 4-methyl sector ketone-beta-D-glucuronide, isopropyl-beta-D-thiopyran galactoside, bile salt, potassium tellurite and cefixime. The chromogenic medium disclosed by the invention can be used for identifying escherichia coli O157:H7 in coliforms rapidly, simply and conveniently.

Description

technical field [0001] The invention relates to a culture medium for microorganism detection, in particular to a chromogenic culture medium for detecting Escherichia coli O157:H7. Background technique [0002] Hemorrhagic Escherichia coli (enterohemorrhage E.Coli, EHEC) is a subtype of Escherichia coli, EHEC is divided into 157, 26, 111 serotypes, the main pathogenic strain is O157:H7, which can cause infectious diarrhea, because it can cause human named for hemorrhagic enteritis. [0003] Except for non-fermentation or slow fermentation of sorbitol, other common biochemical characteristics of EHEC O157:H7 are basically similar to those of Escherichia coli, but there are also some biochemical reactions that are not completely consistent, which are of differential significance. Although EHECO157:H7 has the uidA gene, the β-glucuronidase encoded by it is inactive and cannot decompose 4-methylumbelliferone-β-D-glucuronide (MUG) to produce fluorescence, that is, MUG is negative...

Claims

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Application Information

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IPC IPC(8): C12Q1/10
Inventor 金京勋
Owner SOOCHOW KH BIO SCI & TECH CO LTD KHB
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