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Hybridoma Cell Line Secreting Anti-Chinese Wheat Mosaic Virus Monoclonal Antibody and Application of Monoclonal Antibody

A technology of wheat mosaic virus and hybridoma cell lines, which is applied in antiviral immunoglobulin, analytical materials, biochemical equipment and methods, etc., and can solve the problems of inappropriate field sample detection in large quantities

Active Publication Date: 2017-07-25
ZHEJIANG UNIV
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Problems solved by technology

All of these methods have their limitations and are not suitable for the detection of large quantities of field samples
Serological methods are suitable for batch detection of field samples, but must rely on specific virus monoclonal antibodies

Method used

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  • Hybridoma Cell Line Secreting Anti-Chinese Wheat Mosaic Virus Monoclonal Antibody and Application of Monoclonal Antibody
  • Hybridoma Cell Line Secreting Anti-Chinese Wheat Mosaic Virus Monoclonal Antibody and Application of Monoclonal Antibody

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Embodiment Construction

[0019] The hybridoma cell line 18A3 secreting monoclonal antibodies against Chinese wheat mosaic virus can secrete specific monoclonal antibodies against Chinese wheat mosaic virus. The hybridoma cell line 18A3 was deposited in China Microorganisms on July 3, 2014 General Microorganism Center of the Preservation Management Committee, the deposit number is CGMCC No.9337;

[0020] Anti-Chinese wheat mosaic virus monoclonal antibody ascitic fluid indirect ELISA titer reaches 10 -8 , the antibody type and subclass are IgG1, kappa chain, the monoclonal antibody can have a specific immune binding reaction with the capsid protein of Chinese wheat mosaic virus, and the sensitivity of the monoclonal antibody to detect diseased leaves reaches 1:40960 times dilution;

[0021] The monoclonal antibody against Chinese wheat mosaic virus was specifically reactive only with Chinese wheat mosaic virus, but not with wheat yellow mosaic virus, barley yellow mosaic virus, barley yellow dwarf viru...

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Abstract

The invention discloses a hybridoma cell strain for secreting an anti-Chinese wheat mosaic virus monoclonal antibody and application of the monoclonal antibody of the hybridoma cell strain. A Chinese wheat mosaic virus particle which is purified by using a differential centrifugal method is a hybridoma cell strain 18A3 which is prepared from antigen immune BALB / c mice through cell fusion, screening and cloning, can be stably passaged and secrete a specific and sensitive monoclonal antibody for preventing the Chinese wheat mosaic virus, and the preservation number of the hybridoma cell strain is CGMCCNo.9337. The ascites ELISA titer of the monoclonal antibody secreted from hybridomas is up to 10-8, and the antibody type and the subtype are IgG1 and kappa chains. The monoclonal antibody secreted from 18A3 is subjected to specific reaction with the Chinese wheat mosaic virus but does not react with the wheat yellow mosaic virus, the barley yellow mosaic virus, the rice stripe virus, the tobacco mosaic virus and the like. The hybridoma cell strain and the monoclonal antibody secreted from the hybridoma cell strain provide technical and physical support to diagnosis, detection and scientific prevention and control on wheat virus diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell line secreting anti-Chinese wheat mosaic virus monoclonal antibody and the application of the monoclonal antibody. Background technique [0002] Wheat mosaic disease in China is a disease caused by a virus transmitted by fungi in the soil. The main biological agent of transmission is a lower fungus that lives in the soil, namely Polymyxa graminis Led., the pathogen It is Chinese wheat mosaic virus (CWMV), and often co-infects wheat with wheat yellow mosaic virus (WYMV). Both groups of viruses belong to the group of fungal-borne rhabdoviruses. Observed under an electron microscope, the virion is a straight rod-shaped dyad, that is, it has two shapes: long and short. Nano. [0003] Since the 1970s of the 20th century, polymyxa graminearum and wheat virus diseases transmitted by Polymyxa graminearum have occurred in 9 provinces, including Henan, Sichuan, Hubei, Shaan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C07K16/10G01N33/577G01N33/569
Inventor 吴建祥周雪平谢艳刘欢李娜
Owner ZHEJIANG UNIV
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