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Method for preparing Antarctic krill oil and its microcapsules and low-fluorine Antarctic krill peptide by aqueous enzymatic method

An Antarctic krill oil and Antarctic krill technology, which is applied in the directions of microcapsule preparation, microsphere preparation, fat oil/fat production, etc., can solve difficult problems such as full extraction of Antarctic krill oil, separation of organic solvents, and large usage of organic solvents, etc. The problem is to overcome the incomplete extraction of lipid composition, stable weight and rich variety.

Active Publication Date: 2017-01-25
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When leaching with a single organic solvent, the extraction efficiency is low. When extracting Antarctic krill oil abroad, a strong polar organic solvent and a weak polar organic solvent are used for step-by-step extraction. This method is called "two footwork"
Although the two-step method can improve the extraction efficiency, there are also problems such as cumbersome operation process, large amount of organic solvent used, separation and recovery of organic solvent, etc.
In addition, the desolvation of shrimp oil extracted by organic solvent extraction generally requires high temperature, which will destroy the structure of the heat-sensitive functional active ingredient astaxanthin in Antarctic shrimp oil, and affect the quality of shrimp oil
In addition to organic solvent extraction, there is also a greener extraction method, supercritical CO 2 The extraction method can extract Antarctic krill oil without using organic solvents, but the shrimp oil extracted by this method is mainly neutral oils such as triglycerides, and the content of polar oils - phospholipids is very low, and phospholipids are just The characteristic functional ingredients of Antarctic krill oil, so purely use supercritical CO 2 Extraction Difficult to Achieve Adequate Extraction of Antarctic Krill Oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Heat treatment of Antarctic krill (whole shrimp) in a 100° C. water bath for 30 min, and homogenize.

[0014] (2) Add 1 times water by weight and stir well. Adjust the pH of the homogenate to 8 with 1 mol / L sodium hydroxide solution, add food-grade alkaline protease (EC3.4.21.62), the enzyme dosage is 250U / g krill raw material, and enzymatically hydrolyze it at 40°C for 0.5h . The enzymatic hydrolysis solution was heated to boiling and kept boiling for 10 minutes to inactivate the protease. The enzymatic hydrolyzate was cooled to 10°C and centrifuged at 8000 × g for 10 min. The enzymatic hydrolysis solution after centrifugation can be divided into 4 layers, from top to bottom, they are free oil layer + emulsion layer I, water layer, emulsion layer II, and residue layer. The oil recovery rate of the obtained free oil layer + emulsion layer I and emulsion layer II was 60.65±2.07%, and the phospholipid content accounted for 51.62±1.08% of lipids.

[0015] (3) Colle...

Embodiment 2

[0018] (1) Heat treatment of Antarctic krill (whole shrimp) in a 100° C. water bath for 30 min, and homogenize.

[0019] (2) Add 4 times of water by weight and stir well. Adjust the pH of the homogenate to 10 with 1 mol / L sodium hydroxide solution, add food-grade alkaline protease (EC3.4.21.62), the enzyme dosage is 1000U / g krill raw material, and enzymatically hydrolyze it at 60 ° C for 2.5 hours . The enzymatic hydrolysis solution was heated to boiling and kept boiling for 30min to inactivate the protease. The enzymatic hydrolysate was cooled to 40°C and centrifuged at 13500×g for 30min. The enzymatic hydrolysis solution after centrifugation can be divided into 4 layers, from top to bottom, they are free oil layer + emulsion layer I, water layer, emulsion layer II, and residue layer. The oil recovery rate of the obtained free oil layer + emulsion layer I and emulsion layer II was 61.51 + 0.58%, and the phospholipid content accounted for 61.11 + 0.89% of the lipid.

[002...

Embodiment 3

[0023] (1) Heat treatment of Antarctic krill (whole shrimp) in a 100° C. water bath for 30 min, and homogenize.

[0024](2) Add 2 times of water by weight and stir evenly. Use 1mol / L sodium hydroxide solution to adjust the pH value of the homogenate to 9, add food-grade alkaline protease (EC3.4.21.62), the enzyme dosage is 750U / g krill raw material, and enzymolyze at 50°C for 1.5h . Heat the enzymolysis solution to boiling and keep boiling for 20 minutes to inactivate protease. The enzymolysis solution was cooled to 20°C and centrifuged at 13500×g for 15 minutes. The centrifuged enzymatic hydrolyzate can be divided into 4 layers, from top to bottom are free oil layer + emulsion layer I, water layer, emulsion layer II, and residue layer. The oil recovery rate of the obtained free oil layer + emulsion layer I and emulsion layer II was 64.77±0.12%, and the phospholipid content accounted for 65.58±1.02% of lipids.

[0025] (3) Collect the free oil layer + emulsion layer I and ...

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Abstract

The invention discloses a method for preparing euphausia superba oil, a microcapsule of the euphausia superba oil and low-fluorine euphausia superba peptide by using an aqueous enzymatic method. The method comprises the following steps: thermally treating and homogenizing a euphausia superba raw material, subsequently performing enzymolysis and centrifuging, wherein the centrifuged enzymatic hydrolysate is divided into four layers, respectively including a free oil layer + an emulsion layer I, a water layer, an emulsion layer II and a residue layer from top to bottom; collecting the free oil layer + emulsion layer I and the emulsion layer II, taking the free oil layer + emulsion layer I and the emulsion layer II as core materials, heating and mixing Arabic gum and gelatin to obtain a mixed solution which is taken as a wall material, emulsifying, homogenizing and then performing spray-drying to obtain a euphausia superba microcapsule; filtering the water layer by a ceramic membrane, carrying out vacuum concentration on the filtrate and then spray-drying to obtain low-fluorine euphausia superba peptide powder. The method disclosed by the invention is green, friendly to environment and free of solvent residues, prevents unsafe factors such as solvent residues caused by a solvent method and overcomes the problem of incomplete extraction of lipid compositions in a supercritical fluid extraction method; moreover, the method disclosed by the invention is used for respectively preparing the euphausia superba oil, the euphausia superba microcapsule and the low-fluorine euphausia superba peptide, so that the variety of euphausia superba oil products is enriched.

Description

technical field [0001] The invention relates to the deep processing technology of Antarctic krill, and more particularly, to a method for preparing Antarctic krill oil, its microcapsules and low-fluorine Antarctic krill peptides by an aqueous enzymatic method. Background technique [0002] Antarctic krill (Euphausia superba) is one of the largest and most successful single biological resources on earth. According to the data released by the Food and Agriculture Organization of the United Nations, the biomass of Antarctic krill is about 125-725 million tons, and the annual catch is more than 13 million tons. It is an important potential fishery resource. In recent years, with the gradual depletion of the world's traditional fishery resources and the proposal of the 200-nautical-mile exclusive economic zone, the huge Antarctic krill resources in Antarctic waters have attracted the attention of some developed countries in the distant water fishery. my country has been explorin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/00C11B1/02B01J13/02C12P21/06
CPCB01J13/043B01J13/046C11B1/00C11B1/02C11B1/025C12P21/06Y02P20/54
Inventor 周大勇徐文思宋泽宇潘锦锋朱蓓薇詹佳新王风雅何超琪
Owner DALIAN POLYTECHNIC UNIVERSITY
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