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Polypeptide PP1 capable of promoting expression of growth hormone of tilapia mossambica and application thereof

A tilapia and growth-promoting agent technology, applied in the field of polypeptide function research, can solve problems such as reduced food intake and weight loss, and achieve the effect of promoting growth and good application prospects

Active Publication Date: 2015-02-25
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of reduced food intake and weight loss when feeding tilapia plant protein feed, and to provide a polypeptide that can promote the expression of tilapia growth hormone, thereby promoting the growth of tilapia

Method used

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  • Polypeptide PP1 capable of promoting expression of growth hormone of tilapia mossambica and application thereof
  • Polypeptide PP1 capable of promoting expression of growth hormone of tilapia mossambica and application thereof
  • Polypeptide PP1 capable of promoting expression of growth hormone of tilapia mossambica and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Analysis of Tilapia digestive tract enzymatic hydrolysis products

[0024] The tilapia with uniform body weight of 50±2 grams were selected to be fed commercial compound feed in groups, and the adaptation period was 7 days. They were fed at 9:00 every day, and samples were extracted on the 4th day. After feeding, it was divided into 6 periods of 1.0, 1.5, 3.0, 6.0, 9.0, 12.0, and 24.0 hours, with two fish in each period, and all samples were mixed with the amount of the two fish. Blood was drawn from the tail vein of the fish with a syringe at each time period, and all the blood was extracted and injected into 3.8% (w / v) trisodium citrate dissolved in PBS at a ratio of 1 / 10. Then the tilapia was dissected, and the gastrointestinal tract was taken and washed with normal saline. The tilapia stomach was divided into cardia, pyloric caeca and pylorus, and the intestine was divided into duodenum (pyloric part of stomach to intestinal spiral part), Foregut (the ...

Embodiment 2

[0026] Embodiment 2: Tilapia enzymolysis fragment PP1 Functional studies of - intraperitoneally injected juvenile tilapia

[0027] The polypeptide PP1 was prepared by chemical synthesis according to the sequence obtained by mass spectrometry detection. Dissolve PP1 and positive control protein NPY (neuropeptide Y) in PBS buffer, and inject intraperitoneally into juvenile tilapia. The experiment was divided into negative control group (PBS buffer), PP1 and positive control protein high-dose group (0.24nmol / gbw), PP1 and positive control protein medium-dose group (0.12nmol / gbw) and PP1 and positive control protein low-dose group (0.024nmol / gbw), 10 fish in each group. The fish were anesthetized with eugenol at 2 hours and 6 hours after the injection, and the pituitary tissues were taken out in liquid nitrogen, and then transferred to a -80°C refrigerator for storage until use. RNA was extracted according to the instruction manual of Trizol reagent from Invitrogen Company, ...

Embodiment 3

[0028] Example 3: Polypeptides PP1 Functional studies of - repeated intraperitoneal injections of juvenile tilapia

[0029] According to the results of the first intraperitoneal injection, PP1 and the positive control protein can promote the expression of GH mRNA to a greater extent 2 hours after injection, so the injection experiment was repeated, focusing on the effect of sampling 2 hours after injection on the expression of GH mRNA. The experiments were negative control group, PP1 and positive control protein high dose group (0.24nmol / gbw), PP1 and positive control protein medium dose group (0.12nmol / gbw) and PP1 and positive control protein low dose group (0.024nmol / gbw ), 15 fish in each group. Two hours after the injection, the fish were anesthetized with eugenol, and the pituitary tissue was taken out in liquid nitrogen, and then transferred to a -80°C refrigerator for storage until use. RNA was extracted according to the instruction manual of Trizol reagent from I...

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Abstract

The invention belongs to the technical field of functional study of polypeptide and discloses a polypeptide PP1 capable of promoting expression of growth hormone of tilapia mossambica and application thereof. The amino acid sequence of the polypeptide PP1 is YPVPLENPGPADPAEEL. By intraperitoneal injection of tilapia mossambica by a solution of the polypeptide PP1, results display that the PP1 can obviously promote the expression level of growth hormone in hypophysis cerebri of tilapia mossambica and has physiological activity. The polypeptide PP1 disclosed by the invention can be used for preparing an oral preparation for improving GHmRNA expression of hypophysis cerebri or fish offspring seed growth promoters or additives.

Description

technical field [0001] The invention belongs to the technical field of polypeptide function research, and in particular relates to a polypeptide PP1 capable of promoting the expression of tilapia growth hormone and its application. The application is specifically the application of the polypeptide in promoting the growth of tilapia juveniles. Background technique [0002] Tilapia, commonly known as the longevity fish, belongs to the family Cichlidae and belongs to the genus Oreochromis. Tilapia has the characteristics of miscellaneous food habits, low oxygen resistance, and strong fecundity. It has become an important farmed fish in the world's aquaculture industry and is known as one of the main sources of animal protein in the future. [0003] The demand for tilapia in the international market continues to increase. In the global fish trade, tilapia occupies the third place, second only to salmon and trout, and has extremely high economic value. With the continuous expans...

Claims

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Application Information

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IPC IPC(8): C07K7/08A23K1/18A23K1/16
Inventor 李文笙吴阿敏
Owner SUN YAT SEN UNIV
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