Compounds with tumor cell proliferation resistance, and preparation method and application thereof
A compound, separation and purification technology, applied in the field of medicine, can solve the problem of very little research on symbiotic bacteria and their metabolites
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Embodiment 1
[0029] Example 1: Preparation and structure identification of the compounds chaetomin F and H.
[0030] (1) Preparation of Chaetomycin F and H as shown in formula (1)
[0031] 1. Fermentation conditions
[0032] Preparation of seed culture solution: Take 3.0g of yeast extract, 3.0g of malt extract, 5.0g of peptone, and 10.0g of glucose, dissolve them in an appropriate amount of water, then dilute to 1000mL with water, sterilize at 121°C for 30 minutes, and set aside. The insect symbiotic bacterium Chaetomium globosum TW1-1 was inoculated into the above-mentioned culture medium, and cultured on a shaker under the conditions of 25-28° C. and 100-120 rpm for 3-5 days to obtain a seed culture solution.
[0033] Fermentation: put 200g of rice into a 1000mL Erlenmeyer flask, add 200mL of water, sterilize at 121°C for 30min, and set aside. The above seed culture solution is inoculated into the rice culture medium, and cultured statically for 25-30 days under the condition of 25-28°...
Embodiment 2
[0039] Example 2: Inhibitory activity of compounds 2-9 on leukemia cells HL-60, human liver cancer cells SMMC-7721, human lung cancer cells A-549, human breast cancer cells MCF-7, and human colon cancer cells SW480 cells.
[0040] The antitumor activity of compound 2-9 was tested by MTS method against leukemia cell HL-60, human liver cancer cell SMMC-7721, human lung cancer cell A-549, human breast cancer cell MCF-7, and human colon cancer cell SW480 five human tumor cells Make an evaluation. The results are shown in Table 3:
[0041] Table 1. 1 H NMR data for compounds 1,6,and 8(400MHz,J in Hz)
[0042]
[0043]
[0044]
[0045] Experimental conclusion: Compound 4 selectively inhibits human liver cancer cells SMMC-7721, and can be used as a lead compound for the development of anti-hepatoma cell drugs. Compound 8 has significant inhibitory activity on five human tumor cells, among which it is superior to the positive control drug cisplatin on human lung cancer cell...
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