Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing nucleotide from waste beer yeast

A technology for waste brewer's yeast and nucleotides, which is applied in the field of nucleotide preparation, can solve the problems of low nucleotide yield, low ribonucleic acid extraction rate, etc., achieves high purity, improves yield and purity, and reduces processes. Effect

Inactive Publication Date: 2015-02-18
成都宏安生物科技有限公司
View PDF3 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The salt method has the advantages of mild extraction conditions and low cost, and is widely used. However, compared with the dilute alkali method, the extraction rate of ribonucleic acid is low, so the yield of nucleotides after enzymatic hydrolysis is relatively low, so It is necessary to optimize the salt extraction process to improve the extraction rate and purity of ribonucleic acid, thereby increasing the yield and purity of nucleotides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for preparing nucleotides by waste brewer's yeast, comprising the following steps:

[0037] A. Pretreatment

[0038] Sieve the dried beer waste yeast twice with a 100-mesh sieve, then add 1 volume of sodium bicarbonate with a mass concentration of 1% based on the volume of the beer waste yeast, wash for 0.5h, and then use a centrifuge at a speed of 1000r / Centrifuge for 1 min to obtain yeast sludge, then wash with 1 times the volume of yeast sludge until it has no bitter taste, and then centrifuge with a centrifuge at a speed of 1000r / min to obtain treated yeast sludge;

[0039] B. Extraction and filtration

[0040] The processed yeast slime obtained in step A is formulated into a yeast slime suspension, so that the yeast concentration in the suspension reaches 1% by weight, and then sodium chloride is added to make the sodium chloride concentration in the suspension reach 20% by weight. %, then use sodium hydroxide to adjust the pH value of the suspension to...

Embodiment 2

[0052] A method for preparing nucleotides by waste brewer's yeast, comprising the following steps:

[0053] A. Pretreatment

[0054] Sieve the dried beer waste yeast twice with a 100-mesh sieve, then add 0.1% tartaric acid aqueous solution with a mass concentration of 5 times the volume of the beer waste yeast, wash for 3 hours, and then centrifuge with a centrifuge at a speed of 5000r / min Separation to obtain yeast sludge, and then wash with 5 times the volume of water based on the volume of yeast sludge until there is no bitterness, and then centrifuge with a centrifuge at a speed of 5000r / min to obtain treated yeast sludge;

[0055] B. Extraction and filtration

[0056] The processed yeast slime obtained in step A is formulated into a yeast slime suspension, so that the yeast concentration in the suspension reaches 20% by weight, and then sodium chloride is added to make the sodium chloride concentration in the suspension reach 20% by weight. %, then use sodium hydroxide ...

Embodiment 3

[0068] A method for preparing nucleotides by waste brewer's yeast, comprising the following steps:

[0069] A. Pretreatment

[0070] Sieve the dried beer waste yeast twice with a 100-mesh sieve, then add 0.5% sodium bicarbonate with a mass concentration of 3 times the volume of the beer waste yeast, wash for 1.5 hours, and then use a centrifuge at a speed of 3000r / centrifuge for 1 min to obtain yeast sludge, then wash with 3 times the volume of yeast sludge until it has no bitter taste, and then use a centrifuge to separate at a speed of 3000r / min to obtain treated yeast sludge;

[0071] B. Extraction and filtration

[0072] The processed yeast slime obtained in step A is formulated into a yeast slime suspension, so that the yeast concentration in the suspension reaches 15% by weight, and then sodium chloride is added to make the sodium chloride concentration in the suspension reach 15% by weight. %, then use sodium hydroxide to adjust the pH value of the suspension to 7.5,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing nucleotide from waste beer yeast. The method comprises the following steps: sieving, debittering and deodorizing the waste beer yeast to prepare a yeast suspension, extracting the yeast suspension for a period of time in a stainless steel reactor by virtue of a high-temperature concentrated salt method; after the extraction is completed, rapidly cooling and filtering to obtain supernatant; carrying out ultrafiltration desalting and concentration by virtue of an ultrafiltration device to obtain a concentrated solution, hydrolyzing the concentrated solution with protease, carrying out high-temperature enzyme deactivation and adjusting the acidity so that protein is denatured and precipitated; centrifuging and taking supernatant; purifying the supernatant through an ultrafiltration device, adding a nuclease for hydrolyzing, carrying out enzyme deactivation, purifying and drying through a spray dryer to obtain yeast-nucleotide powder. Compared with the prior art, since the high-temperature concentrated salt extraction method, the proteolysis technology and the ultrafiltration membrane technology are adopted, the method is easily operated and low in cost, the extraction rate and purity of the nucleotide are increased, the yeast-nucleotide powder prepared by the method is high recovery rate and purity, the value-adding capacity of processing of the waste beer yeast is increased and the huge economic benefits are generated.

Description

technical field [0001] The invention relates to a method for preparing nucleotides, in particular to a method for preparing nucleotides by using beer waste yeast. Background technique [0002] Beer waste yeast is a by-product of beer production. It is produced after the main fermentation and post-fermentation brewing process in the beer production process. It contains rich nutrients and functional components. Beer waste yeast contains about 40% to 50% protein, 4% to 6% ribonucleic acid, 30% to 35% carbohydrate, and 2.8% to 3% lipid. Ribonucleic acid has been widely used in medicine, health care products, agriculture, food processing, etc. The four 5-nucleotides obtained by hydrolysis of ribonucleic acid have broad application prospects in the pharmaceutical industry as basic raw materials. Nucleotides have a variety of biological functions in organisms, and play an important role in maintaining cell structure and regulating cell energy. In recent years, with the developme...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P19/30
Inventor 郝刚
Owner 成都宏安生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com