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Application of PCBP1 gene in preparing radiotherapy sensitivity enhancing kit

A technology of 1. PCBP1 and kits, applied in gene therapy, genetic material components, drug combinations, etc., can solve the problems of no reports

Inactive Publication Date: 2015-02-11
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So is PCBP1 a new target for tumor sensitization? There are no reports in this area at home and abroad

Method used

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  • Application of PCBP1 gene in preparing radiotherapy sensitivity enhancing kit
  • Application of PCBP1 gene in preparing radiotherapy sensitivity enhancing kit
  • Application of PCBP1 gene in preparing radiotherapy sensitivity enhancing kit

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Example 1. The radiation synergistic effect of PCBP1 and heavy ion radiation on human cervical cancer HeLa tumors

[0045] 1. Experimental materials

[0046] Human cervical cancer HeLa cells were initially purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (catalogue number: TCHu187), and then cultured and preserved in our laboratory; D-MEM medium dry powder, calf serum, and trypsin powder were purchased from White Gibco Company; Propidium iodide (PI) was purchased white Jiangsu Biyuntian Biotechnology Research Institute; Dimethyl sulfoxide (DMSO), agarose and Tween-20 were purchased white Shanghai High-tech Bioengineering Co., Ltd.; anti-PCBP1, Tap73 Antibodies such as , and DeltaNp73 were purchased from Santa Cruzs Biotechnology Company of the United States. Lipofectamine TM In 2000, the transfection reagent was purchased from Invitrogen Company of the United States. The cellular experimental material...

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Abstract

The invention discloses application of a PCBP1 gene in preparing a radiotherapy sensitivity enhancing kit, and in particular relates to application of the PCBP1 gene in preparing a sensitivity enhancing kit for tumor radiotherapy utilizing ionizing radiation including heavy ion rays. The nucleotide sequence of the PCBP1 gene is as shown in SEQ ID NO.1 and the PCBP1 gene is established in eukaryotic expression plasmid. Together with heavy ion beams, the PCBP1 gene regulates and controls expression of the CD44v6 gene related to tumor metastasis and invasion, inhibits proliferation, migration and invasion of HeLa tumor cells, remarkably down-regulates the cell cycle regulatory protein Cyclin D1, prompts cells to generate G0 / G1 retardation, enhances the radiotherapy sensitivity of HeLa cells, up-regulates pro-apoptotic protein Tap73 and expression of the mitochondria apoptosis pathway MCL, down-regulates the apoptosis inhibition DelNp73 protein, and induces apoptosis of many HeLa cells.

Description

technical field [0001] The present invention relates to a new application of polycytosine binding protein 1 (poly C binding protein1, PCBP1, or heterogeneous nuclear ribonuclear protein E1, hnRNP E1 or α-complex protein1, αCP1) gene, and specifically relates to the use of PCBP1 gene in the preparation of radiosensitizing drugs in the application. Background technique [0002] Cervical cancer is one of the common female reproductive tract malignancies in my country. The incidence of cervical cancer has become the fastest-growing cancer in large and medium-sized cities, and the age of onset is gradually becoming younger. It has become one of the diseases that seriously endanger the health of women in my country. [0003] Radiation therapy is one of the routine means of tumor treatment, and 70% to 80% of malignant tumor patients need to receive radiotherapy during the course of treatment. Like chemotherapy, radiotherapy is an important palliative treatment. According to stri...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61P35/00
Inventor 狄翠霞张红周鑫孙超司婧甘露刘圆圆
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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