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Polypeptide molecule and application thereof

A molecular and polypeptide technology, applied in the field of polypeptide molecules and its application, can solve the problems of a large number of antibiotic treatment, high infection rate, removal of implants, etc., and achieve the effect of preventing bacterial infection, obvious application potential, and strong stability.

Active Publication Date: 2015-02-04
合肥瑞克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Implant materials are mainly used in the treatment of dentition defects and bone loss. This treatment has a high infection rate, which may lead to serious consequences such as massive antibiotic treatment, removal of implants and even death.

Method used

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  • Polypeptide molecule and application thereof
  • Polypeptide molecule and application thereof
  • Polypeptide molecule and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A polypeptide molecule, the amino acid sequence of which is shown in SEQ ID No.1;

[0026] SEQ ID No. 1: KKPVPIIYCN KKRGKCQRM.

Embodiment 2

[0028] Preparation of polypeptide molecules:

[0029] Select amino acid-king resin as the carrier (resin), fully swell the resin with dichloromethane, wash several times with dimethylformamide, use DBLK of appropriate concentration to remove the Fmoc-protecting group, and then use dimethylformamide to remove the Fmoc-protecting group. The amide was washed several times to remove DBLK, and the appropriate condensing agent benzotriazole-N, N, N', N'-tetramethyluronium hexafluorophosphate, the activator methylmorpholine and the C-terminal Two Fmoc-protected amino acids (Fmoc-Pro-OH) are coupled, detected by ninhydrin detection method to ensure that the connection is relatively complete, washed several times with dimethylformamide, washed away various residual residues, and activated Reagent and condensing agent, coupled according to the amino acid sequence, after connecting all the amino acids, remove the last Fmoc-protecting group, cleavage with cutting fluid, remove the resin a...

Embodiment 3

[0031] Antibacterial ability test:

[0032] 1. Experimental method

[0033] Add 1mL concentration of 1×10 to a sterilized test tube 6 1 mg of the polypeptide molecule provided by the present invention was added to each / mL bacterial solution, and after culturing at 37°C for 24 hours, the culture medium was collected and diluted in multiples, the dilution factor was 10 times, and the number of viable bacteria was detected by the coating culture method.

[0034] 2. Experimental results:

[0035] Table 1 The bactericidal rate (%) of the polypeptide molecule of the present invention

[0036] bacteria

[0037] As can be seen from Table 1, the polypeptide molecules provided by the present invention are effective against Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922), Candida albicans (ATCC 10231), Bacillus subtilis black variety (ATCC 9372 ) have strong bactericidal properties, with a bactericidal rate of over 99%.

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Abstract

The invention discloses a polypeptide molecule including: (a) an amino sequence of the polypeptide molecule, wherein the amino sequence is represented as the SEQ ID No.1; and (b) a polypeptide molecule which is derived from (a) and has same biological functions of the polypeptide molecule (a), wherein the polypeptide molecule (b) is obtained since one or more amino acids are deleted, inserted or replaced in the polypeptide molecule limited in (a). A molecular weight of the polypeptide molecule is 2289.91 g / mol and an isoelectric point of the polypeptide molecule is 11.1. The polypeptide molecule can be applied for preparing materials which is anti-bacterial and can promote growth of osteoblast. The invention provides the polypeptide molecule which can be compounded with other compounds simply, can be applied in materials in various types and has effects of promoting growth of the osteoblast and being anti-bacterial. The polypeptide molecule is free of toxic and harmful effect on human body. The polypeptide molecule is simpler in synthetic method compared with a large-molecular polypeptide and can be industrially produced.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a polypeptide molecule and its application. Background technique [0002] Dental implants are implanted in the missing teeth of the human body. According to their materials, they are divided into metal and alloy materials, ceramic materials, carbon materials, polymer materials, and composite materials. Implant materials are mainly used in the treatment of dentition defects and bone loss. This treatment has a high infection rate, which may lead to serious consequences such as massive antibiotic treatment, removal of implants and even death. There are two main reasons for the high incidence of implant-associated infection: the accumulation of bacterial plaque on the implant surface and the low resistance of the implant osseointegration interface. In order to avoid implant-related infection, the graft should have two excellent properties: excellent antibacterial properties and osteoblast...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K6/02
Inventor 李培源苏炜霍丽妮陈睿
Owner 合肥瑞克生物科技有限公司
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