Method for extracting limonin analogues from tangerine seeds

Abstract

The invention discloses a method for extracting limonin analogues from tangerine seeds. The method comprises the following steps: firstly, obtaining raw materials, wherein the raw materials are subjected to ultrasonic extraction with 90% ethanol for 1 hour to obtain an extract, the extract is directly detected, the content of limonin is required to be equal to or greater than 0.6% and the content of nomilin is equal to or greater than 0.3%; secondly, breaking hulls of the raw materials and pulverizing until the hulls of the raw materials are completely broken; thirdly, extracting the raw materials with 90% ethanol for three times and 1.5 hours each time and ultimately controlling that the active ingredients in the residue account for no more than 3% of the raw material total target, wherein the ratio of material to solvent is 1 to 4 to 4 to 3; fourthly, concentrating the extract until the degree of alcohol is 20%, standing for 12 hours and removing floating oil on the surface of the liquid; fifthly, continuously recovering the solvent from a concentrated solution, controlling the extraction specific gravity to be 1.03+ / -0.1, standing and crystallizing for 12 hours; sixthly, extracting an upper layer supernatant, filtering the lower layer precipitate with filter cloth and centrifuging; and seventhly, after centrifuging, carrying out coarse crystallization and vacuum drying at a temperature of less than 80 DEG C.

Description

technical field [0001] The invention belongs to the technical field of extraction of natural medicines, and in particular relates to the use of mandarin orange pit as a raw material, extraction by methanol heat reflux, impurity-removing treatment of the extract, concentration of the extract with a specific specific gravity to precipitate coarse crystals, and degreasing and separation to obtain limonoid analogues The crude product can be obtained by recrystallization to obtain limonin essence. [0002] Background technique [0003] Limonoids (Limlclis) are a class of triterpenoids present in Rutaceae and Meliaceae plants. In recent years, studies have shown that limonoids can strongly activate the activity of the main detoxification enzyme in the body - glutathione-transferase (GSH-transferase). A large number of animal experiments have shown that limonoids have the ability to inhibit the Liver cancer, small intestine cancer, oral cancer and other cancers are a class of ph...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a new approach for the development and utilization of orange stones, to reduce the waste of raw materials and environmental pollution, and to provide a high-efficiency extraction and separation of limonin and nomi The Craft of Forest Composition

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