A lipopeptide-kaolin compound biocontrol preparation and its preparation method and application
A technology of biocontrol agent and kaolin, which is applied in the field of microbial source pesticide preparation, can solve the problems of low compounding degree, poor stability, and short effect time, and achieve the effects of prolonging the action time, saving production costs, and improving the control effect
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Embodiment 1
[0040] The preparation method of lipopeptide-kaolin compound biocontrol preparation: (1) preparation of lipopeptide powder: Streak the frozen preserved Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain XZ-173 (preservation number CGMCCNO.4160) on the LB plate, Put it in an incubator for activation, pick a single colony and inoculate it in LB liquid medium, culture it at 30°C and 170rpm for 24 hours to obtain a fermented seed liquid; weigh 4.5kg of soybean powder, 3kg of straw, 0.15kg of starch and 0.15kg of Put the yeast extract into a 50L fermentation tank, add 1L of inorganic salt solution, adjust the pH of the substrate to 7, ensure the water content is 40%, set the speed at 50rpm and stir for 60min to fully mix the substrate and sterilize; among them, the inorganic salt solution Including (g / L):KH 2 PO 4 1.0, MgSO 4 ·7H 2 O 0.5, KCl 0.5, L-phenylalanine 2×10 -3 , MnSO 4 5×10 -3 , CuSO 4 0.16×10 -3 , FeSO 4 0.15×10 -3 After the temperature in the ...
Embodiment 2
[0044] The preparation method of lipopeptide-kaolin compound biocontrol agent: (1) preparation of lipopeptide powder: the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) bacterial strain XZ-173 that freezes preservation is streaked on LB plate, is placed in the incubator and activated, Pick a single colony and inoculate it in LB liquid medium, at 30°C, 170rpm
[0045] Cultivate under conditions for 24 hours to obtain fermented seed liquid; weigh 6kg of soybean powder, 4.5kg of straw, 0.2kg of starch and 0.2kg of yeast extract, put them into a 50L fermenter, add 2L of inorganic salt solution, adjust the pH of the substrate to 8, and contain water The rate is 60%, set the rotating speed to 70rpm and stir for 80min to fully mix the matrix and sterilize; wherein, the inorganic salt solution includes (g / L): KH 2 PO 4 1.0, MgSO 4 ·7H 2 O 0.5, KCl 0.5, L-phenylalanine 2×10 -3 , MnSO 4 5×10 -3 , CuSO 4 0.16×10 -3 , FeSO 4 0.15×10 -3 ; After the temperature in the ...
Embodiment 3
[0049] The preparation method of lipopeptide-kaolin compound biocontrol agent: (1) preparation of lipopeptide powder: the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) bacterial strain XZ-173 that freezes preservation is streaked on LB plate, is placed in the incubator and activated, Pick a single colony and inoculate it in LB liquid medium, cultivate it at 30°C and 170rpm for 24 hours, and use it as a fermented seed solution; weigh 5.67kg of soybean flour, 3.67kg of straw, 0.17kg of starch and 0.18kg of yeast extract, and put In a 50L fermenter, add 1L of inorganic salt solution, adjust the pH of the matrix to 7.5, and the water content to 55%, set the rotation speed to 70rpm and stir for 60 minutes to fully mix the matrix and sterilize; wherein, the inorganic salt solution includes (g / L): K H 2 PO 4 1.0, MgSO 4 ·7H 2 O 0.5, KCl 0.5, L-phenylalanine 2×10 -3 , MnSO 4 5×10 -3 , CuSO 4 0.16×10 -3 , FeSO 4 0.15×10 -3After the temperature in the fermenter i...
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