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Working electrode biological reactant and application and electrode-type test strip thereof

A working electrode and biological reaction technology, applied in the field of electrochemical detection, can solve the problems of increasing detection cost and increasing operation difficulty, and achieve the effect of avoiding interference

Inactive Publication Date: 2015-01-28
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above detection methods all use serum as the analysis sample. Before the detection, pretreatment steps such as centrifugation, sedimentation, and separation are required to eliminate possible sources of interference. However, the portable rapid self-test system cannot perform such pretreatment steps and needs to be modified. Use whole blood to detect ascorbic acid content analysis system, which will inevitably increase the cost of detection and increase the difficulty of operation
[0004] So far, there is no portable rapid self-test system that can be used to detect the content of AA in whole blood, so it is of great significance to develop a real-time detection and analysis system for AA in whole blood that is suitable for self-monitoring

Method used

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  • Working electrode biological reactant and application and electrode-type test strip thereof
  • Working electrode biological reactant and application and electrode-type test strip thereof
  • Working electrode biological reactant and application and electrode-type test strip thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Embodiment 1 The preparation of working electrode and reference electrode bioreaction membrane liquid

[0076] The formulation of the working electrode bioreaction membrane solution is shown in Table 1.

[0077] Table 1 The formula of the working electrode bioreaction membrane liquid

[0078]

[0079]

[0080] Take 80.85mL ultrapure water, add 8g methylcellulose, 5g lactose and 0.05g Triton-100, stir for 4 hours and dissolve completely. Then, 4 g of potassium ferrocyanide and 0.1 g of sodium sulfite were added, stirred and dissolved. Finally, 1 g of ascorbate oxidase (216 U / mg, purchased from Amano, Japan) and 1 g of peroxidase (254 U / mg, purchased from Japan Toyobo) were added, stirred and dissolved to obtain the working electrode bioreaction membrane solution.

[0081] The formulation of the reference electrode bioreaction membrane solution is shown in Table 2.

[0082] Table 2 The formula of the reference electrode bioreaction membrane liquid

[0083]

...

Embodiment 2

[0085] Embodiment 2 Preparation of working electrode and reference electrode bioreaction membrane liquid

[0086] The formulation of the working electrode bioreaction membrane solution is shown in Table 3.

[0087] Table 3 The formula of the working electrode bioreaction membrane liquid

[0088]

[0089]

[0090] Take 94.22mL ultrapure water, add 1g methylcellulose, 1g lactose and 0.03g Triton-100, stir for 4 hours and dissolve completely. Then, 0.5 g of potassium ferrocyanide and 0.5 g of sodium sulfite were added, stirred and dissolved. Finally, 0.25 g of ascorbate oxidase (216 U / mg, purchased from Amano, Japan) and 2.5 g of peroxidase (254 U / mg, purchased from Japan Toyobo) were added, stirred and dissolved to obtain the working electrode bioreaction membrane liquid.

[0091] The formulation of the reference electrode bioreaction membrane solution is shown in Table 4.

[0092] Table 4 The formula of reference electrode bioreaction membrane liquid

[0093]

[00...

Embodiment 3

[0095] Embodiment 3 Preparation of working electrode and reference electrode bioreaction membrane liquid

[0096] The formulation of the working electrode bioreaction membrane solution is shown in Table 5.

[0097] Table 5 The formula of the working electrode bioreaction membrane liquid

[0098]

[0099]

[0100] Take 68.15mL ultrapure water, add 10g methylcellulose, 10g lactose and 0.1g Triton-100, stir for 4 hours and dissolve completely. Then, 8 g of potassium ferrocyanide and 1 g of sodium sulfite were added and stirred to dissolve. Finally, 2.5 g of ascorbate oxidase (216 U / mg, purchased from Amano, Japan) and 0.25 g of peroxidase (254 U / mg, purchased from Japan Toyobo) were added, stirred and dissolved to obtain the working electrode bioreaction membrane liquid.

[0101] The formula of the reference electrode bioreaction membrane solution is shown in Table 6.

[0102] Table 6 The formula of reference electrode bioreaction membrane liquid

[0103]

[0104] Ta...

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Abstract

The invention relates to the technical field of electrochemical detection, in particular to a working electrode biological reactant and application and an electrode-type test strip thereof. The working electrode biological reactant comprises ascorbic acid oxidase, peroxidase and a reductive electronic mediator. The electrode-type test strip provided by the invention can accurately determinate the content of ascorbic acid in whole blood and can avoid interference of endogenous dehydrogenized ascorbic acid in serum.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to a working electrode biological reactant and its application, and an electrode type test strip. Background technique [0002] Ascorbic acid (AA), also known as vitamin C and L-ascorbic acid, is an important small molecular organic compound necessary for maintaining life in animals and plants. The human body cannot synthesize ascorbic acid by itself and needs to ingest it from the outside world. AA participates in many biochemical reactions in the body, such as removing harmful free radicals in the body, promoting the biosynthesis of collagen antibodies, nerve mediators, and hormones. When the human body lacks ascorbic acid, it can lead to scurvy, low immunity, colds, and even induce cancer. In addition, excessive intake of AA can also cause pathological symptoms such as urinary stones, diarrhea, and stomach cramps. Therefore, detecting the content of AA in blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 黄钊蔡晓华
Owner SINOCARE
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