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Degradation bacterium for oil-base drilling cuttings, and preparation method and application method thereof

A technology for oil-based drilling cuttings and degrading bacteria, which is applied in biochemical equipment and methods, chemical instruments and methods, and microorganism-based methods, etc. The effect of short processing cycle and simple operation

Active Publication Date: 2015-01-28
CHINA PETROCHEMICAL CORP +1
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Problems solved by technology

The existing microbial treatment method for oil-based drilling cuttings is an anaerobic composting-aerobic degradation-planting process. The treatment cycle is generally 180d, or even more than 360d, and the total petroleum hydrocarbon (THP) content can be reduced to less than 1%. There are defects that the degradation rate is not high in a short period of time and the processing cycle is long

Method used

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  • Degradation bacterium for oil-base drilling cuttings, and preparation method and application method thereof

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preparation example Construction

[0025] The preparation of medium is listed in Table 1:

[0026] Table 1

[0027]

Embodiment 1

[0029] (1) Take petroleum-contaminated soil at the well site, carry out enrichment culture on the bacteria in the soil under the conditions of LB medium, neutral environment, and temperature at 30°C, and isolate, purify and screen the bacteria after enrichment culture, Three strains of degrading monobacteria were obtained, including Pseudomonas aeruginosa, Micrococcus krusei, and Acinetobacter calcoaceticus.

[0030] (2) Mix the three strains of degrading monobacteria obtained in step 1 according to the volume ratio of Pseudomonas aeruginosa: Micrococcus krusei: Acinetobacter calcium acetate 1:0.9:0.9 to obtain a mixed bacterial solution, in which oil-based drilling cuttings The viable count of degrading bacteria is 10 9 ~10 11 pcs / g.

[0031] (3) The mixed bacterial liquid of step (2) is fermented and amplified in the industrial medium P1, and the fermentation process conditions are: 33° C. of fermentation temperature, 10% v / v of inoculum size, 100 rpm of stirring speed, 24...

Embodiment 2

[0034] Oil-contaminated soil was taken at the well site, and the bacteria in the soil were enriched and cultured in LB medium, neutral environment, and temperature at 35°C. The bacteria after enrichment culture were separated, purified and screened to obtain 3 strains Degradative monobacteria, including Pseudomonas aeruginosa, Micrococcus krusei, and Acinetobacter calcoaceticus.

[0035] The same step as in Example 1, the difference is that Pseudomonas aeruginosa: Micrococcus krusei: Acinetobacter calcium acetate is mixed in a volume ratio of 1:1:1; the mixed bacteria solution is carried out in industrial medium P3 Fermentation scale-up cultivation; fermentation process conditions are: fermentation temperature is 35 ℃, inoculum size is 15% v / v, stirring speed is 120rpm, fermentation time is 36h, and the gas-liquid volume ratio of feeding air per minute is 1.2; after fermentation, Filter and separate the degrading bacteria through a plate and frame filter press with a pressure ...

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Abstract

The invention discloses a degradation bacterium for oil-base drilling cuttings, and a preparation method and an application method thereof, and belongs to the technical field of microbiological processing of oil-containing solid wastes. The degradation bacterium for oil-base drilling cuttings is composed of multiple microorganisms, and is prepared by performing fermentation culturing on three degradation single bacteria which are obtained by performing enriching, separation, purification and screening on bacteria in petroleum polluted soil. The degradation bacterium for oil-base drilling cuttings comprises pPseudomonas aeruginosa, kocuria kristinae and acinetobacter calcoaceticus, is used for degradation of petroleum composition in oil-base drilling cuttings, and has the substantial advantages of high oil removal efficiency and short processing period. When oil-base drilling cutting with the initial oil content less than 5% is processed by using the degradation bacterium for 5-30 d, the oil content can be reduced to 1% or less, and the oil removal rate can reach 80% or more. The degradation bacterium also has good application prospect when being applied to biological restoration of oil-containing soil and oil-containing sludge.

Description

technical field [0001] The invention relates to an oil-based drilling cuttings degrading bacterium and a preparation and application method thereof, belonging to the technical field of microbial treatment of oily solid waste. Background technique [0002] Oil-based drilling cuttings in oily solid wastes have been listed as national hazardous wastes. Because of their abnormally stable system, complex types of pollutants, and high oil content, it is very difficult to deal with them. Among oil-based drilling cuttings treatment technologies, biological treatment technology has become an important means of safe treatment and disposal of oil-based drilling cuttings on site because of its advantages of safety, economy, high efficiency, and no secondary pollution, especially for those that cannot be removed by mechanical devices. Pore ​​oil and thin oil layer, while limiting the use of chemical agents, the use of biological treatment technology can show greater advantages. The exis...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A62D3/02C12R1/385C12R1/01
CPCB09C1/10C02F11/02C02F2101/32C12N1/20C12N1/205C12R2001/385
Inventor 单海霞何焕杰王中华马金王芹詹适新
Owner CHINA PETROCHEMICAL CORP
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