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Detection kit for early diagnosis of systemic lupus erythematosus

A detection kit, lupus erythematosus technology, applied in the field of molecular biology, can solve the problems of increasing the risk of death and delaying the disease

Inactive Publication Date: 2015-01-21
SHANGHAI QY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Misdiagnosis will not only cause a double burden on the health and economy of patients, but the most important thing is that it will delay the treatment and increase the risk of death

Method used

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  • Detection kit for early diagnosis of systemic lupus erythematosus
  • Detection kit for early diagnosis of systemic lupus erythematosus
  • Detection kit for early diagnosis of systemic lupus erythematosus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Use of detection kit

[0030] 1. Extract sample DNA

[0031] Clean the oral cavity, use a disinfectant scraper to scrape the oral mucosa of the subject to collect epithelial cells, and extract genomic DNA by the phenol-chlorine method.

[0032] 2. PCR extension reaction

[0033] Use the PCR reaction reagent in the kit, which contains the following primer pairs:

[0034] (1) CR2 (rs17615) forward primer: SEQ NO1

[0035] CR2 (rs17615) reverse primer: SEQ NO2

[0036] (2) CR2 (rs3813946) forward primer: SEQ NO3

[0037] CR2 (rs3813946) reverse primer: SEQ NO4

[0038] The PCR amplification reaction system is: 10XPCR reaction buffer 10μl; 10mM dNTP mixture 2μl; 5U / μl Taq DNA polymerase 1μl; specific primers 1μl each; sterile deionized water 73μl

[0039] PCR reaction program: 95 degrees Celsius for 3 minutes pre-denaturation, 94 degrees Celsius for 30 seconds for denaturation, cooling to 55 degrees Celsius for 35 seconds for annealing, then heating to 72 degrees Celsius for 40...

Embodiment 2

[0058] Example 2. Provide molecular diagnostic services for patients with systemic lupus erythematosus

[0059] 1. Sampling and extracting genome

[0060] The hospital laboratory doctor uses a human saliva extraction kit to extract a sample of epithelial cells from the oral cavity of the subject and extract DNA.

[0061] 2. Genotyping

[0062] By using the method described in Example 1 and using the kit provided by the present invention, DNA sequences containing rs17615, rs1048971 and rs3813946 sites in the CR2 gene of the subject were obtained, and the genotype of the sites was determined.

[0063] 3. Determine whether the patient has or the possibility of developing systemic lupus erythematosus later

[0064] According to the genotype of the subject, a diagnosis report of systemic lupus erythematosus is issued. The report details the detection results and mutation types of rs17615, rs1048971 and rs3813946 sites on the CR2 gene of the subject, and calculates and issues evaluation resul...

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Abstract

The invention provides a kit for detection of 3 SNP mutation loci, i.e. rs3813946 of the human CR2 gene 5' UTR region and rs17615 and rs104897 on a tenth exon. The kit can be applied in early diagnosis of systemic lupus erythematosus (SLE), and can realize early discrimination of SLE by detection of patients' genotype combination on a molecular level. The method provided by the invention is a sequencing genotyping method, has an accuracy rate up to 99.9%, and is easy for wide use and popularization.

Description

Technical field [0001] The invention belongs to the field of molecular biology and relates to a detection kit for the early diagnosis of systemic lupus erythematosus. Background technique [0002] Systemic lupus erythematosus (SLE) is an autoimmune-mediated, diffuse connective tissue disease characterized by immune inflammation, which involves multiple systems, multiple organs, complex clinical manifestations, and protracted course of autoimmune disease. . This disease mostly occurs in women of childbearing age. There are about one million patients in my country, and it has a trend of increasing year by year. The average prevalence is the second highest in the world. [0003] However, the clinical misdiagnosis rate of systemic lupus erythematosus is quite high. This is mainly because systemic lupus erythematosus is caused by the interaction of multiple factors such as heredity, infection, sex hormones, and environment. The cause of the disease is still unknown. Although the Ameri...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 刘骁
Owner SHANGHAI QY BIOTECH CO LTD
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