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Imprinting material with strong affinity and recognition capability on celery butyl-phthalide and preparation method of imprinting material

An imprinted material, apigenin A technology, applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., can solve problems such as inability to purify with trace substances, low efficiency of apigenin A, poor selectivity, etc. , to achieve the effect of simple operation, high affinity and simple instrument conditions

Inactive Publication Date: 2015-01-21
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, traditional separation techniques (solvent extraction, fractional distillation, steam distillation, adsorption, etc.) have been used to extract and separate apigenin A from celery seeds, which have the disadvantages of low efficiency, time-consuming, and waste. The modern extraction and separation technologies mainly include: ultrasonic technology, macroporous adsorption resin technology, ultrafiltration technology, enzyme engineering technology, microwave extraction technology, semi-bionic extraction method, ionic liquid extraction, supercritical fluid extraction technology, high-speed countercurrent chromatographic separation, etc. Although these techniques have improved the extraction efficiency of apigenin to a certain extent, their selectivity is poor and cannot be used for the purification of trace substances.

Method used

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  • Imprinting material with strong affinity and recognition capability on celery butyl-phthalide and preparation method of imprinting material
  • Imprinting material with strong affinity and recognition capability on celery butyl-phthalide and preparation method of imprinting material
  • Imprinting material with strong affinity and recognition capability on celery butyl-phthalide and preparation method of imprinting material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: An imprinted material (MIPs) with high affinity and high recognition ability for apigenin A. The mass percentages of the components required for the preparation of the imprinted material are: apigenin A 0.225%, acrylamide 0.168%, ethylene glycol di Methacrylate 4.694%, azobisisobutyronitrile 0.190%, porogen 94.723%.

[0035] The porogen is acetone.

[0036] Its preparation method is as follows:

[0037] A. Add 0.225% apigenin A, 0.168% acrylamide and 94.723% porogen acetone in the reaction kettle by mass percentage. After ultrasonic dissolution at room temperature for 5 minutes, let it stand for 4 hours to make it fully pre-polymerized;

[0038] B. After pre-polymerization, add 4.694% ethylene glycol dimethacrylate and 0.190% azobisethylbutyronitrile respectively, ultrasonically degas for 5 minutes, then fill with nitrogen for 15 minutes, seal, and place at 60 °C Constant temperature reaction in water bath for 24 hours;

[0039] C. The prepared imprinted...

Embodiment 2

[0042] Example 2: An imprinted material (MIPs) with high affinity and high recognition ability for apigenin A. The mass percentages of the components required for the preparation of the imprinted material are: apigenin A 0.224%, acrylamide 0.503%, ethylene glycol di Methacrylate 4.678%, azobisisobutyronitrile 0.190%, porogen 94.405%.

[0043] The porogen is acetone.

[0044] Its preparation method is as follows:

[0045] A. Add 0.224% apigenin A, 0.503% acrylamide and 94.405% porogen acetone in the reaction kettle by mass percentage, and after ultrasonic dissolution at room temperature for 5 minutes, let it stand for 4 hours to make it fully pre-polymerized;

[0046] B. After pre-polymerization, add 4.678% ethylene glycol dimethacrylate and 0.190% azobisethylbutyronitrile respectively by mass percentage, ultrasonically degas for 5 minutes, then fill with nitrogen for 15 minutes, seal and place at 60 °C Constant temperature reaction in water bath for 24 hours;

[0047] C. Th...

Embodiment 3

[0050] Example 3: An imprinted material (MIPs) with high affinity and high recognition ability for apigenin A. The mass percentages of the components required for the preparation of the imprinted material are: apigenin A 0.233%, acrylamide 0.348%, ethylene glycol di Methacrylate 1.214%, azobisisobutyronitrile 0.197%, porogen 98.008%.

[0051] The porogen is acetone.

[0052] Its preparation method is as follows:

[0053] A. Add 0.233% apigenin A, 0.348% acrylamide and 98.008% porogen acetone in the reaction kettle by mass percentage, and after ultrasonic dissolution at room temperature for 5 minutes, let stand for 4 hours to make it fully pre-polymerized;

[0054] B. After pre-polymerization, add 1.214% ethylene glycol dimethacrylate and 0.197% azobisethylbutyronitrile respectively, ultrasonically degas for 5 minutes, then fill with nitrogen for 15 minutes, seal and place at 60 °C Constant temperature reaction in water bath for 24 hours;

[0055] C. The prepared imprinted...

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Abstract

The invention relates to an imprinting material with strong affinity and recognition capability on celery butyl-phthalide and a preparation method of the imprinting material. The material can be prepared by the following steps: firstly, adding celery butyl-phthalide, acrylamide and a pore foaming agent-acetone into a reaction kettle and performing ultrasound dissolution on the mixture at the room temperature for 5 minutes, leaving the mixture to stand for 4 hours for complete pre-polymerization to obtain pre-polymerization mixed liquid; then, respectively adding ethylene glycol dimethacrylate and azo-bis-iso-butyrynitrile into the pre-polymerization mixed liquid, carrying out ultrasound degassing for 5 minutes, filling nitrogen for 15 minutes, sealing and placing the mixed liquid into water bath under a constant temperature of 60 DEG C to react for 24 hours to obtain imprinting polymerization; eluting the mixed liquid utilizing mixed solution of ethyl alcohol and acetic acid till no template molecule celery butyl-phthalide is detected in the eluting liquid; finally, washing away surplus ethyl acid utilizing ethyl alcohol, drying the eluted imprinting polymerization under vacuum at 60 DEG C for 24 hours and discharging. The imprinting material provided by the invention has high affinity capability on celery butyl-phthalide, overcomes defects of low-efficiency, time consumption, material waste and the like of traditional separation technology extracting celery butyl-phthalide from celery seeds.

Description

technical field [0001] The invention relates to the technical field of molecular imprinting, which is a method of forming a complex through a non-covalent bond between a template molecule and a functional monomer in a suitable solvent, and then adding a cross-linking agent and an initiator to cause a polymerization reaction to occur, and then from the generated The imprinted material with three-dimensional holes that is completely complementary to the size, shape, and functional groups of the template molecule can be obtained by removing the template molecule from the polymer. This material can be applied to solid-liquid phase extraction and separation. Background technique [0002] The active ingredients of traditional Chinese medicine have the characteristics of complex structure, various types, low content and instability, so it is very difficult to separate and purify active active ingredients from Chinese herbal medicine. At present, traditional separation methods such ...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F220/56C08J9/26B01J20/26B01J20/28B01J20/30
Inventor 谭倪张稳颜雪明武亚新贾晓鹤侯丹廖森杨雪纯程易
Owner NANHUA UNIV
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