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Method for extracting and purifying calcium lactate

A calcium lactate and phase-separation technology, applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of difficult recovery, high separation cost, complicated process, etc., and achieve low production cost, high impurity removal rate, and process Simple and practical effect

Active Publication Date: 2015-01-21
郑州运维生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the organic solvents used to extract lactic acid in the fermentation broth by the extraction method are toxic, easily polluted, and difficult to recycle, and the extraction process requires stripping operation, which is complicated
Although the salting-out extraction method has high extraction efficiency, it is difficult to separate salt and high-concentration lactic acid, and the separation cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for extracting refined calcium lactate in the present embodiment may further comprise the steps:

[0026] (1) Take the L-calcium lactate fermentation broth and centrifuge to remove the bacteria to obtain a calcium lactate solution with a concentration of 160g / L;

[0027] (2) Adjust the pH value of the calcium lactate solution to 5. Add the phase-splitting solvent methyl acetate and ethanol to the calcium lactate solution at 10°C. The volume ratio of the calcium lactate solution to methyl acetate and ethanol is 2:3:1.2. After uniformity, let it stand for 5 minutes to separate the phases, take out the upper phase and put it in the crystallization tank for later use, and the extraction rate of calcium lactate in one extraction is 57.3%;

[0028] (3) Remove the lower phase for secondary extraction. Add phase-splitting solvents methyl acetate and ethanol to the lower phase at 30°C. The volume ratio of the lower phase to methyl acetate and ethanol is 2:2:0.7. Set ...

Embodiment 2

[0031] (1) Take the L-calcium lactate fermentation broth and centrifuge to remove the bacteria to obtain a calcium lactate solution with a concentration of 120g / L;

[0032] (2) Adjust the pH value of the calcium lactate solution to 5. Add the phase-separation solvent ethyl acetate and ethanol to the calcium lactate solution at 60°C. The volume ratio of the calcium lactate solution to ethyl acetate and ethanol is 1:1:1. After uniformity, let stand to separate the phases, take out the upper phase and put it in the crystallization tank for standby, the extraction rate of calcium lactate in one extraction is 82%;

[0033] (3) Remove the lower phase for secondary extraction. Add the phase-splitting solvent ethyl acetate and ethanol to the lower phase at 60°C. The volume ratio of the lower phase to ethyl acetate and ethanol is 1:0.7:0.6. Set the phase separation, take out the upper phase and put it in the crystallization tank. After the second extraction, the total extraction rate o...

Embodiment 3

[0036] (1) Take the L-calcium lactate fermentation broth and centrifuge to remove the bacteria to obtain a calcium lactate solution with a concentration of 140g / L;

[0037] (2) Adjust the pH value of the calcium lactate solution to 5. Add the phase-splitting solvent methyl acetate and methanol to the calcium lactate solution at 30°C. The volume ratio of the calcium lactate solution to methyl acetate and methanol is 2:3:1. After uniformity, let stand to separate the phases, take out the upper phase and put it in the crystallization tank for standby, the extraction rate of calcium lactate in one extraction is 63.8%;

[0038] (3) Remove the lower phase for secondary extraction. Add phase-splitting solvents methyl acetate and methanol to the lower phase at 50°C. The volume ratio of the lower phase to methyl acetate and methanol is 2:2:0.6. Set the phase separation, take out the upper phase and put it in the crystallization tank. After the second extraction, the total extraction ra...

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Abstract

The invention discloses a method for extracting and purifying calcium lactate, and belongs to the technical field of biochemical engineering. The method comprises the following steps of removing impurities from a calcium lactate solution by centrifugation; adding a phase-separating solvent, mixing uniformly, standing for separating phases, taking an upper phase, crystallizing to obtain calcium lactate, wherein the phase-separating solvent is acetonitrile or a mixed solution formed by any one of acetonitrile, ethyl formate, methyl formate, methyl acetate, ethyl acetate, tetrahydrofuran and methyl ethyl ketone and any one of C1-C3 alcohol and C3-C5 alcohol. An extraction-crystallization method is adopted to extract and purify calcium lactate; calcium lactate can be crystallized naturally in the phase-separating solvent; a re-extraction step is omitted; and the process is simple and practicable. The phase-separating solvent has a low boiling point, can be recycled easily, has no toxicity or low toxicity, and is safe and environment-friendly. By adopting the method provided by the invention to extract and purify calcium lactate, a removal rate of impurities is high; a removal rate for pigment, protein and total sugar can reach 97%, 98% and 98% respectively; and the calcium lactate can be used for preparing high-purity lactic acid. The method is particularly suitable for calcium lactate fermentation broth prepared by straw hydrolysate with high impurity content.

Description

technical field [0001] The invention specifically relates to a method for extracting and refining calcium lactate, which belongs to the technical field of biochemical industry. Background technique [0002] At present, the industrial production of L-lactic acid mostly adopts the fermentation method. Due to the influence of impurities such as residual sugar, pigment and protein, the extraction of refined L-lactic acid from the fermentation broth has always been a bottleneck restricting the production cost of lactic acid. The extraction of refined lactic acid at home and abroad mainly adopts calcium salt method and organic solvent extraction method. Calcium salt method is the method most used by domestic manufacturers. Calcium carbonate is added to react with lactic acid in the fermentation process to adjust the pH value of the system. , concentrated to obtain high-purity lactic acid. The production of lactic acid by this method consumes a lot of waste, the amount of waste w...

Claims

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Application Information

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IPC IPC(8): C07C59/08C07C51/48C07C51/43
CPCC07C51/43C07C51/48C07C59/08
Inventor 吴健王平高慧姣戴桂馥
Owner 郑州运维生物技术有限公司
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