Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ginsenoside Rg3 poly(lactic-co-glycolic acid) nano microsphere and preparation method thereof

A technology of polylactic acid glycolic acid and ginsenoside, which is applied in the field of medicine, can solve the problems of low blood drug concentration, limited clinical drug efficacy, and reduced drug utilization rate, and achieve the effects of reducing side effects, good therapeutic effect, and reducing the number of medications

Inactive Publication Date: 2015-01-21
BEIJING JIAOTONG UNIV
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, 20(R)-ginsenoside Rg3 is not easily soluble in water. Through pharmacokinetic studies on ginsenoside Rg3, it has been found that after oral administration, Rg3 absorbs quickly, metabolizes quickly, and has low blood drug concentration, which reduces its drug utilization. Greatly limit its clinical efficacy, but also limit the application of its parenteral route of administration

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ginsenoside Rg3 poly(lactic-co-glycolic acid) nano microsphere and preparation method thereof
  • Ginsenoside Rg3 poly(lactic-co-glycolic acid) nano microsphere and preparation method thereof
  • Ginsenoside Rg3 poly(lactic-co-glycolic acid) nano microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] like figure 1 As shown, the preparation method of ginsenoside Rg3 polylactic-glycolic acid nano-microspheres according to an embodiment of the present invention comprises the following steps:

[0031] S1: Dissolve ginsenoside Rg3 in dimethyl sulfoxide (DMSO) to obtain the first solution of the first predetermined concentration, and dissolve polylactic acid-glycolic acid copolymer in dichloromethane (DCM) to obtain the second predetermined concentration. concentration of the second solution.

[0032] S2: adding a predetermined ratio of the first solution to the second solution, and ultrasonicating to form a suspension.

[0033] S3: Add the suspension into an aqueous solution containing polyvinyl alcohol (PVA), and perform ultrasonic emulsification to obtain an emulsion.

[0034] S4: drying the emulsion, and after the microspheres of the emulsion are solidified, centrifuge, wash, and freeze-dry to obtain ginsenoside Rg3 polylactic-glycolic acid nanospheres.

[0035] Ac...

Embodiment 1

[0044] Example 1: Firstly, ginsenoside Rg3 was dissolved in DMSO with a drug concentration of 40 mg / ml, and PLGA was dissolved in DCM with a concentration of 30 mg / ml. Precisely measure 1ml of PLGA solution, add 80μl of ginsenoside Rg3 solution to it, ultrasonic power 210w, supersonic for 5 seconds, pause for 5 seconds, and ultrasonic for 1 minute.

[0045] The suspension was quickly added to 10 ml of aqueous solution containing 1% PVA, and the aforementioned ultrasonic conditions were repeated, and the resulting emulsion was poured into 30 ml of aqueous solution containing 1% PVA, and after 3 hours of magnetic stirring at 1000 r, the microspheres were separated by high-speed freezing and centrifugation. Washed three times with deionized water, freeze-dried and stored at -20°C. Microsphere scanning electron microscope photo as figure 2 As shown in A.

Embodiment 2

[0046] Example 2: Firstly, ginsenoside Rg3 was dissolved in DMSO with a drug concentration of 40 mg / ml, and PLGA was dissolved in DCM with a concentration of 30 mg / ml. Precisely measure 1ml of PLGA solution, add 60μl of ginsenoside Rg3 solution to it, ultrasonic power 210w, supersonic for 5 seconds, stop for 5 seconds, and ultrasonic for 1 minute.

[0047] The suspension was quickly added to 10 ml of aqueous solution containing 1% PVA, and the aforementioned ultrasonic conditions were repeated, and the resulting emulsion was poured into 30 ml of aqueous solution containing 1% PVA, and after 3 hours of magnetic stirring at 1000 r, the microspheres were separated by high-speed freezing and centrifugation. Washed three times with deionized water, freeze-dried and stored at -20°C. Microsphere scanning electron microscope photo as figure 2 Shown in B.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a ginsenoside Rg3 poly(lactic-co-glycolic acid) (PLGA) nano microsphere and a preparation method thereof. The preparation method of the ginsenoside Rg3 poly(lactic-co-glycolic acid) nano microsphere comprises the following steps: S1, dissolving ginsenoside Rg3 in dimethyl sulfoxide to obtain a first liquid with a first predetermined concentration, and dissolving poly(lactic-co-glycolic acid) copolymer into dichloromethane to obtain a second liquid with a second predetermined concentration; S2, adding the first liquid in a predetermined proportion into the second liquid to perform ultrasonic treatment to form a turbid liquid; S3, adding the turbid liquid into an aqueous liquid containing polyvinyl alcohol to perform ultrasonic emulsion to obtain emulsion; and S4, drying the emulsion, centrifugally separating and washing, and freeze-drying the emulsion after curing the microspheres of the microsphere of the emulsion to obtain the ginsenoside Rg3 poly(lactic-co-glycolic acid) nano microsphere. According to the preparation method of the ginsenoside Rg3 poly(lactic-co-glycolic acid) nano microsphere of the embodiment of the invention, the prepared ginsenoside PLGA microsphere is good in pesticide effect.

Description

Technical field [0001] The present invention relates to the field of medical technology, and more specifically, to ginsenoside Rg3 polylactic acid glycolic acid nanospheres and a preparation method thereof. Background technique [0002] Ginsenoside Rg3 is a tetracyclic triterpenoid saponin compound present in ginseng, with a molecular weight of 784.13. It has two optical isomer forms: 20(R)-ginsenoside Rg3 and 20(s)-ginsenoside Rg3, among which 20(R)-Ginsenoside Rg3 has stable chemical properties and its structural formula is as follows: [0003] [0004] Ginsenoside Rg3 has various pharmacological effects, which can significantly improve the immune function of tumor-bearing mice; inhibit the formation of new blood vessels in tumors; prevent the implantation of exfoliated cancer cells in the blood vessel wall; inhibit the infiltration of tumor cells into the basement membrane of the blood vessel wall, It has significant anti-infiltration and anti-metastasis effects. Cli...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K31/704A61K47/34A61P37/04A61P35/00
Inventor 孙维敏金玺园
Owner BEIJING JIAOTONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com