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Flow polymerase chain reaction (PCR) circulating heating apparatus based on capillaries and heating method

A cyclic heating and chain reaction technology, applied in chemical instruments and methods, specific-purpose bioreactors/fermenters, heating or cooling equipment, etc., can solve the problem of uneven heating of samples, low repeatability, and easy leakage and other problems, to achieve the effect of easy and uniform temperature distribution, good repeatability, and improved heat transfer rate

Active Publication Date: 2015-01-07
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production of microchips has the characteristics of handicrafts, that is, strong individuality and low repeatability; secondly, the chips are made by bonding, which is prone to leakage; the channel of the microchip is rectangular, and the capillary of the existing detection equipment is circular. At the interface between the pipe and the chip, the droplet is in frequent contact with the wall of the channel, and droplet fusion and sample contamination often occur; finally, this method is heating on one side and cooling on the other side, so the sample is heated unevenly

Method used

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  • Flow polymerase chain reaction (PCR) circulating heating apparatus based on capillaries and heating method
  • Flow polymerase chain reaction (PCR) circulating heating apparatus based on capillaries and heating method
  • Flow polymerase chain reaction (PCR) circulating heating apparatus based on capillaries and heating method

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Embodiment 1

[0023] A capillary-based flow polymerase chain reaction spiral heater of the present invention:

[0024] The whole device is composed of a bracket (1), a heating plate (2), a constant temperature channel (3), a capillary tube (5) and a temperature controller (6). Each side of the triangular bracket (1) has a channel (1-1); the constant temperature channel is composed of two identical modules (3-1, 3-2) that are buckled together. The modules (3-1, 3) -2) It is in the shape of a rectangular parallelepiped, with a row of semicircular capillary channels (3-3) engraved on one side of the modules (3-1, 3-2), the capillary (5) is a flexible pipe, and the temperature controller (6) ) consists of three temperature controllers (6-1, 6-2, 6-3), which independently control the temperature of each heating plate;

[0025] The heating plate (2) and the module (3-1) are installed in sequence in each channel (1-1) on the side of the bracket (1); the module (3-1) has the capillary channel (3-3...

Embodiment 2

[0030] The triangular bracket is made of plexiglass, 20mm thick, 80mm wide and 8mm deep. The heating plate adopts an aluminum plate heater, the power supply is 24V DC power supply, and the power is 20W. A single module of the constant temperature channel is an aluminum block, with a thickness of 4mm, a length of 80mm, and a width of 20mm. The diameter of the semicircular channel is 0.8mm and the spacing is 0.2mm. The capillary adopts a silicone tube with an outer diameter of 0.8 mm. The temperature controller purchases the REX-C700 temperature controller. Three groups of heating groups, two for heating and one for cooling, constitute two-temperature PCR. All components are integrated on a flat panel and finally enclosed in a case.

Embodiment 3

[0032] The triangular bracket is made of plastic, 20mm thick, 80mm wide and 8mm deep. The heating plate adopts stainless steel electric heater, the power supply is 24V DC power supply, and the power is 20W. A single module of the constant temperature channel is made of stainless steel, with a thickness of 4mm, a length of 80mm and a width of 20mm. The diameter of the semicircular channel is 0.8mm and the spacing is 0.2mm. The capillary adopts a silicone tube with an outer diameter of 0.8 mm. The temperature controller purchases the REX-C700 temperature controller. The temperature of each group is controlled and displayed by an independent PID controller; three groups of heating groups, two for heating, control 65 and 95 degrees respectively; the remaining group is used for cooling (using a fan), which constitutes a two-temperature PCR. All components are integrated on a flat panel and finally enclosed in a case.

[0033] The capillary-based flow polymerase chain reaction (P...

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Abstract

The invention discloses a flow polymerase chain reaction (PCR) circulating heating apparatus based on capillaries and a heating method. According to the method, PCR of samples in the capillaries is achieved by adopting a method that the capillaries penetrate through three different constant-temperature areas circularly, and a new process that amplification is achieved in a three-dimensional flow process of the samples under the condition of static temperature control can be formed. In a temperature cycling process, a fluid in each capillary carries sample droplets to flow into one end of a heating device, then penetrates among the three temperature areas along the capillary, and flows out from the heating device after completing a predetermined number of heating cycles to enter a detection device. Compared with a planar micro-chip method, the heating method disclosed by the invention is easily combined with existing biological detection equipment, and meanwhile, because the capillaries with standard batch production are adopted, the capillaries are higher in repeatability of working performance, are very suitable for being used as plugins, and can perform combined operation with the existing high-precision biological detection equipment.

Description

technical field [0001] The invention belongs to the technical field of microfluidic temperature control, and in particular relates to a capillary-based flow polymerase chain reaction (PCR) cyclic heating apparatus and a method for cyclic heating in the apparatus. Background technique [0002] The polymerase chain reaction (PCR) uses DNA to become single-stranded when it is denatured in vitro at a high temperature of 95°C. When the temperature is low (often around 60°C), the primer and the single-strand are combined according to the principle of base complementary pairing, and then the temperature is adjusted. To the optimum reaction temperature of DNA polymerase (about 72°C), DNA polymerase synthesizes complementary strands along the direction of phosphate to five-carbon sugar (5'-3'). The PCR instrument based on polymerase is actually a temperature control device, which can be well controlled between the denaturation temperature, the renaturation temperature and the extensi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/34
CPCB01L7/525B01L7/54B01L2300/0838
Inventor 何立群焦冬生罗昭锋
Owner UNIV OF SCI & TECH OF CHINA
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