Method for performing low-dosage radiant biology early warning by utilizing luminous bacteria

A luminescent bacteria and low-dose technology, applied in the direction of dosimeters, etc., can solve problems such as comprehensively responding to radiation pollution, and achieve the effects of low environmental risk, short time, and simple equipment

Inactive Publication Date: 2014-12-17
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of methods for using luminescent bacteria to detect and comprehensively evaluate the hazards of low-dose radiation, and to conduct biological early warning, and traditional low-dose radiation detection and evaluation methods cannot quickly, effectively and comprehensively deal with various complex radiation pollution conditions , to assess the comprehensive toxicity of low-dose radiation and to carry out biological early warning

Method used

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  • Method for performing low-dosage radiant biology early warning by utilizing luminous bacteria
  • Method for performing low-dosage radiant biology early warning by utilizing luminous bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Add 5 mL of 0.85% NaCl solution to 5 mg of Vibrio Qinghai freeze-dried powder, mix well, and place it at 25°C for 15 min to restore the stable luminescence of Vibrio Qinghai to obtain the luminescent bacteria recovery solution. Add 2 mL of 0.85% NaCl solution to 10 measuring cups, and then add 0.5 mL of luminous bacteria resuscitating solution, mix them well, place them at 25 °C for 15 min, and take 5 of them to measure The cup was placed in a gamma-ray radiation environment with a dose rate of 0.055 Gy / min for 11 s, and the cumulative radiation dose received at this time was 10 mGy. After 20 min, the luminous intensity of the luminescent bacteria in the control group and the radiation group was measured with a luminescent bacteria detector, and the photoelectron readings were recorded. According to the average value of photoelectron readings, the luminous intensity inhibition rate of luminescent bacteria in the radiation group was calculated, and the value was 12.0%. A...

Embodiment 2

[0040] Add 5 mL of 0.85% NaCl solution to 5 mg of Vibrio Qinghai freeze-dried powder, mix well, and place it at 25 °C for 15 min to restore the stable luminescence of Vibrio Qinghai to obtain the luminescent bacteria recovery solution. Add 2 mL of 0.85% NaCl solution to 10 measuring cups, and then add 0.5 mL of luminous bacteria resuscitating solution to make them fully mixed. After standing at 25 °C for 15 min, take 5 of them to measure The cup was placed in a gamma-ray radiation environment with a dose rate of 0.055 Gy / min for 15 s, and the cumulative radiation dose received at this time was 14 mGy. After 20 min, the luminous intensity of the luminescent bacteria in the control group and the radiation group was measured with a luminescent bacteria detector, and the photoelectron readings were recorded. According to the average value of photoelectron readings, the luminous intensity inhibition rate of luminescent bacteria in the radiation group was calculated, and the value w...

Embodiment 3

[0042] Add 5 mL of 0.85% NaCl solution to 5 mg of Vibrio Qinghai freeze-dried powder, mix well, and place it at 25 °C for 15 min to restore the stable luminescence of Vibrio Qinghai to obtain the luminescent bacteria recovery solution. Add 2 mL of 0.85% NaCl solution to 10 measuring cups, and then add 0.5 mL of luminous bacteria resuscitating solution to make them fully mixed. After standing at 25 °C for 15 min, take 5 of them to measure The cup was placed in a gamma-ray radiation environment with a dose rate of 0.055 Gy / min for 17 s, and the cumulative radiation dose received at this time was 16 mGy. After 20 min, the luminous intensity of the luminescent bacteria in the control group and the radiation group was measured with a luminescent bacteria detector, and the photoelectron readings were recorded. According to the average value of photoelectron readings, the luminous intensity inhibition rate of luminescent bacteria in the radiation group was calculated, and the value w...

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Abstract

The invention relates to a method for performing low-dosage radiant biology early warning by utilizing luminous bacteria. According to the method, a dosage-effect relationship between a radiant dosage acceptable by the bacteria and a luminous intensity can be established by utilizing a sensitivity of the luminous bacteria to the low-dosage radiation, and the luminous intensity of the bacteria has positive correlation with the activity of luminous elements such as fluorescein, luciferase and ATP in the bacterial cells. Therefore, through calculation of a luminous intensity suppression ratio of the luminous bacteria, the comprehensive toxicity of the low-dosage radiation for the luminous bacteria can be estimated, and a biology early warning method is established. The method for performing low-dosage radiant biology early warning by utilizing the luminous bacteria has multiple advantages of being convenient, simple and fast to operate, low in cost, high in sensitivity, good in accuracy, low in environment risk and the like.

Description

technical field [0001] The invention belongs to the technical field of low-dose radiation biological early warning, and in particular relates to a technology for low-dose gamma radiation biological early warning by using luminescent bacteria. Background technique [0002] According to statistics, the accumulated uranium waste rock in the world has exceeded 40 billion tons, and uranium tailings have exceeded 20 billion tons, which is the largest source of radioactive pollution in uranium mining and metallurgy systems. There are more than 180 solid waste storage sites such as uranium waste rocks and uranium tailings in China, which are distributed in more than 30 counties and cities in 14 provinces and regions across the country. 80% of uranium waste rock and 90% of uranium tailings are distributed in Hunan, Jiangxi and Guangdong. These areas have abundant rainfall and are densely populated. Uranium waste rock and uranium tailings contain 238 U 234 U 232 Th, 226 Ra, 210...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01T1/02
Inventor 丁德馨赵维超龙鼎新胡南李广悦王浩胡淼王永东
Owner NANHUA UNIV
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