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Salt-resisting compound bacterium agent for disposing kitchen waste as well as preparation method and application thereof

A technology of composite bacterial agent and kitchen waste, which is applied in the preparation of organic fertilizers, methods based on microorganisms, biochemical equipment and methods, etc., to achieve the effects of reducing production costs, high activity, and reducing content

Active Publication Date: 2014-12-17
HANYU GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above three problems must be overcome at the same time in order to achieve long-term use of one-time inoculation. However, the existing domestic compost bacteria usually cannot meet the requirements, so the service life is often not more than 3 months

Method used

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  • Salt-resisting compound bacterium agent for disposing kitchen waste as well as preparation method and application thereof
  • Salt-resisting compound bacterium agent for disposing kitchen waste as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] This example is the preparation example of composite bacterial agent.

[0026] (1) Preparation of composite bacteria

[0027] 1) Incline cultivation:

[0028] Bacillus subtilis was inoculated on nutrient agar slant medium, Bacillus coagulans was inoculated on MRS slant medium, and Saccharomyces cerevisiae was inoculated on PDA slant medium. Bacillus subtilis and Bacillus coagulans were cultured at 37° C. for 48 hours, and Saccharomyces cerevisiae was cultured at 28° C. for 72 hours to activate the strains.

[0029] 2) Primary seed liquid culture:

[0030] Inoculate the activated Bacillus subtilis, Bacillus coagulans, and Saccharomyces cerevisiae obtained in step 1 into 250 mL liquid medium respectively. Bacillus subtilis and Bacillus coagulans were cultured at 37° C. for 48 hours, and Saccharomyces cerevisiae was cultured at 28° C. for 48 hours to activate the strains.

[0031] 3) Fermentation tank culture:

[0032] Inoculate the primary seed liquid obtained in ste...

Embodiment 2

[0042] This example is the preparation example of bacterial agent.

[0043] (1) Preparation of composite bacteria

[0044] Cultivate each strain separately according to the cultivation method of Example 1, then collect the cells by centrifugation, and mix them according to the following parts by mass to make a wet powder composite cell, Bacillus subtilis: Bacillus coagulans: Saccharomyces cerevisiae = 2:1: 1.

[0045] (2) Preparation of carrier excipients

[0046] Mix soybean meal powder, corn flour, sugar powder, and wood shavings in parts by mass of 4:2:2:0.8 to make a carrier auxiliary material, and the total weight of the carrier auxiliary material is the same as that of the wet powder composite bacteria.

[0047] (3) Preparation of composite bacterial agent

[0048] Mix the wet powder compound bacteria with half of the carrier auxiliary material, dry to a moisture content of 15%, and then mix with the other half of the carrier auxiliary material. In this way, the bact...

Embodiment 3

[0050] This example is the preparation example of bacterial agent.

[0051] (1) Preparation of composite bacteria

[0052] Cultivate each bacterial species separately according to the cultivation method in Example 1, then collect the thalline by centrifugation, and mix the following mass parts to make a wet powder basic treatment thallus, wherein: Bacillus subtilis: Bacillus coagulans: Saccharomyces cerevisiae=2 :1.2:0.8.

[0053] Further, add functional bacteria and high-efficiency plastic-degrading bacteria to the basic treatment bacteria to prepare composite bacteria. In parts by mass, the amount of functional bacteria added is 20% of the basic treatment bacteria.

[0054] (2) Preparation of carrier excipients

[0055] Rice bran, peanut bran, sugar powder, and bagasse powder are mixed in parts by mass of 4:1:1:1 to make carrier auxiliary materials, and the total weight of the carrier auxiliary materials is the same as that of the wet powder composite bacteria.

[0056] (...

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Abstract

The invention discloses a salt-resisting compound bacterium agent for disposing kitchen waste. The compound bacterium agent is composed of a compound thallus and a carrier auxiliary material, wherein the compound thallus comprises a basic treatment thallus; the basic treatment thallus is composed of bacillus subtilis, bacillus coagulans and saccharomyces cerevisiae; bacillus subtilis in the basic treatment thallus accounts for 30%-65% of a total viable count; bacillus coagulans thallus accounts for 20%-65% of the total viable count; and saccharomyces cerevisiae accounts for 5%-50% of the total viable count. The compound bacterium agent disclosed by the invention is suitable for a kitchen waste disposer with heat-insulation, air-exchanging and agitating functions; and a stable micro-ecology is formed by microorganisms in the bacterium agent and the compound bacterium agent can resist high salt, so that residues obtained by disposing the kitchen waste can be used as the bacterium agent to continually dispose the kitchen waste for the next time, and the compound bacterium agent can be used for a long period when being added at a time.

Description

technical field [0001] The invention relates to the technical field of garbage treatment, in particular to a method and bacterial agent for kitchen garbage treatment. Background technique [0002] With the development of my country's economy and the acceleration of urbanization, the discharge of urban domestic waste is increasing, and the proportion of kitchen waste is increasing day by day, accounting for 37-62% of urban domestic waste. Since food waste contains a lot of sugar, starch, protein, and high salt and oil content, if it is not treated, food waste is easy to deteriorate, rot, breed harmful microorganisms and mosquitoes, and produce a lot of toxins and Distributing malodorous gas will seriously affect the appearance of the city, damage the health of residents, and destroy the quality of the environment. However, food waste is rich in nutrients, and if it can be effectively treated, it will become a resource with certain utilization value. [0003] Existing method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C05F17/00C05F9/04C12R1/865C12R1/125C12R1/07
CPCY02A40/20Y02W30/40
Inventor 梁嘉亮
Owner HANYU GRP CO LTD
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