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Microbial fertilizer granule, microbial fertilizer granule carrier and preparation method of microbial fertilizer granule

A technology of microbial fertilizers and granules, applied in fertilizer forms, fertilizer mixtures, fertilization devices, etc., can solve problems such as unstable colonization, and achieve the effect of prolonging the maintenance of integrity, prolonging the time of maintaining integrity, and improving soil nutrients.

Active Publication Date: 2014-12-17
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The object of the present invention is to provide a kind of novel microbial fertilizer granule, this microbial fertilizer granule has overcome the deficiency that the microorganism in the existing microbial fertilizer can not stably colonize in the soil, and beneficially to the waste biological material that is difficult to handle in agricultural production reuse

Method used

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  • Microbial fertilizer granule, microbial fertilizer granule carrier and preparation method of microbial fertilizer granule
  • Microbial fertilizer granule, microbial fertilizer granule carrier and preparation method of microbial fertilizer granule
  • Microbial fertilizer granule, microbial fertilizer granule carrier and preparation method of microbial fertilizer granule

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: the basic support component and content of microbial fertilizer:

[0054] Crop stalks and sawdust were pulverized with a multifunctional pulverizer with a 5mm mould. Mix the two by mass 1:1, and add clay after mixing evenly. The clay described in the present invention may be any soil other than sandy soil, which acts to bind the mixture. The optimized experimental data of adding clay ratio is shown in Table 1:

[0055] Table 1: The cellulose content of the basic support of microbial fertilizer granules under different ratios

[0056]

[0057] Wherein, when the cellulose ratio reaches 50%, the microbial fertilizer particles can be bonded optimally while the cellulose addition ratio reaches the maximum value.

[0058] Subsequently, the ratio between straw and sawdust was further adjusted, and the results showed that the ratio between straw and sawdust can be selected in a wide range, and the specific ratio will not significantly affect the support mixt...

Embodiment 2

[0061] Embodiment 2: Bacteria antagonism experiment in the microbial fertilizer granule

[0062] In actual application, microbial fertilizer particles are small and closed. In order to determine whether multiple strains in the particles can coexist for a long time, it is necessary to conduct antagonism experiments between different strains. Those that do not produce antagonists with other added strains can be applied in microbial fertilizers.

[0063] The experimental method is that an activated logarithmic growth phase strain absorbs 200ul of bacterial liquid and evenly spreads it on ordinary 1 / 2R 2 A: on solid medium; then draw 2 ul of the second logarithmic growth phase strain and drop it on the above-mentioned coated medium (several spots can be dropped according to the size of the petri dish); culture for 5-7 days. Observe whether there is a zone of inhibition near the bacterial droplet of the second bacterial strain. If no zone of inhibition is produced, it is prelimina...

Embodiment 3

[0065] Embodiment 3: detection of the co-growth status of mixed bacterial strains

[0066] The different bacterial strains to be added to the granules were cultured in liquid medium, and when the OD value of the bacterial liquid was between 0.6 and 0.8, the different cultured bacterial strains were added to the new medium in equal amounts for cultivation. Samples were collected on the 1st, 3rd, 5th, 7th, and 10th day of culture, and denaturing gradient gel electrophoresis (DGGE) was performed to analyze the growth status of different strains under mixed culture conditions. Experiments have proved that different strains can grow and reproduce well under the condition of mixed culture.

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Abstract

The invention relates to a microbial fertilizer granule which comprises a core layer and a coating layer, wherein the core layer comprises the following materials in parts by weight: 4-8 parts of crushed straws, 2-6 parts of crushed wood chips, 10-12 parts of clay and two strains of each of cellulose degradation bacteria, azotobacteria and phosphate solubilizing bacteria, which are uniformly mixed, wherein the quantity of each strain is more than 1*(10<5>-10<6>) in every gram of core layer mixture; and the coating layer is formed by drying 3%-6wt% of a gelatin solution coating the outer side of the core layer in the shade. The invention also relates to a preparation method of the microbial fertilizer granule and a corresponding microbial fertilizer granule carrier. The microbial fertilizer granule disclosed by the invention overcomes the defect of the traditional microbial fertilizer that microbes cannot be stably colonized in soil and is capable of profitably recycling waste biological materials which are difficult to dispose in agricultural production.

Description

technical field [0001] The invention relates to the field of fertilizers in agricultural production, in particular to a microbial fertilizer granule, a granule carrier and a preparation method thereof. Background technique [0002] With the continuous growth of food demand, the use of chemical fertilizers in agricultural production is generally increasing. However, the application of large amounts of chemical fertilizers to farmland will cause water, soil and air pollution. The reason is that it is impossible for all types and forms of chemical fertilizers applied on farmland to be absorbed and utilized by plants. In terms of fertilizer utilization efficiency, nitrogen is 30-60%, phosphorus is 2-25%, and potassium is 30-60%. Various compounds that are not used by plants in time will be transferred to the root dense layer with the infiltrating soil water and cause pollution. As a result, eutrophication of rivers, lakes and inland seas can be caused; soil is polluted and phy...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G5/00
Inventor 张胜男巩瑞红孟建宇梁允刚冯福应
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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