Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Canine distemper and canine parvovirus disease bivalent vaccine and preparation method thereof

A technology of canine parvovirus and dual vaccine, which is applied in the preparation of canine parvovirus dual vaccine, canine parvovirus dual vaccine, and canine distemper, and can solve unsatisfactory immune effect, unstable quality, and adverse reactions To achieve the effect of significant immune effect, less adverse reactions, and reduce the number of emergency responses

Inactive Publication Date: 2014-12-17
ZHENGZHOU HOUYI PHARMA
View PDF1 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a dual vaccine against canine distemper and canine parvovirus disease, which solves the problems of short immunization time, many adverse reactions and unsatisfactory immunization effects caused by the unstable quality of existing vaccines.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The canine distemper and canine parvovirus dual vaccine of this embodiment is mixed with attenuated canine distemper virus vaccine (commercially available) and canine parvovirus attenuated vaccine (commercially available) at a volume ratio of 1:1, and then It is made by adding lyoprotectant and bacteria inhibitor.

[0019] The lyoprotectant is prepared by mixing sucrose and skim milk, sterilizing at 110° C. for 40 minutes, and testing for sterility. The added amount of the lyoprotectant is to make it account for 10% of the total volume of the dual live vaccine. The bacteria inhibitors are penicillin and streptomycin. The amount of the mixed bacteria inhibitor added is: 100 units of penicillin and 200 μg of streptomycin are contained in each milliliter of the dual live vaccine.

[0020] The preparation method of the canine distemper and canine parvovirus dual vaccine of the present embodiment comprises the following steps:

[0021] 1) Fully mix canine distemper virus ...

Embodiment 2

[0024] The canine distemper and canine parvovirus dual vaccine of this embodiment is mixed with attenuated canine distemper virus vaccine (commercially available) and canine parvovirus attenuated vaccine (commercially available) at a volume ratio of 1:1, and then It is made by adding lyoprotectant and bacteria inhibitor.

[0025] The freeze-drying protective agent is prepared by mixing sucrose and skim milk, sterilizing at 120° C. for 30 minutes, and testing for sterility. The added amount of the lyoprotectant is to make it account for 10% of the total volume of the dual live vaccine. The bacteria inhibitors are penicillin and streptomycin. The amount of the mixed bacteria inhibitor added is: 200 units of penicillin and 100 μg of streptomycin per milliliter of the dual live vaccine.

[0026] The preparation method of the canine distemper and canine parvovirus dual vaccine of the present embodiment comprises the following steps:

[0027] 1) Fully mix canine distemper virus a...

Embodiment 3

[0030] The canine distemper and canine parvovirus dual vaccine of this embodiment is mixed with canine distemper virus attenuated vaccine and canine parvovirus attenuated vaccine in a ratio of 1:1 by volume, and then added lyoprotectant and miscellaneous bacteria made of inhibitors.

[0031] The lyoprotectant is prepared by mixing sucrose and skim milk, sterilizing at 115° C. for 35 minutes, and testing for sterility. The added amount of the lyoprotectant is to make it account for 10% of the total volume of the dual live vaccine. The bacteria inhibitors are penicillin and streptomycin. The added amount of the mixed bacteria inhibitor is: 150 units of penicillin and 150 μg of streptomycin are contained in each milliliter of the dual live vaccine.

[0032] The canine distemper virus attenuated vaccine is a recombinant Newcastle disease LaSota attenuated vaccine of canine distemper virus nucleocapsid protein, and the recombinant Newcastle disease LaSota attenuated vaccine is ex...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a canine distemper and canine parvovirus disease bivalent vaccine and a preparation method thereof. The bivalent vaccine is prepared by compounding a canine distemper vaccine and a canine parvovirus disease vaccine according to a volume ratio of 1:1, and then adding a freeze-dried protective agent and a multi-bacteria inhibitor into the mixed vaccine. The provided canine distemper and canine parvovirus disease bivalent vaccine is prepared by compounding a canine distemper vaccine and a canine parvovirus disease vaccine, at the same time a freeze-dried protective agent and a multi-bacteria inhibitor are added to enhance the effect, and the bivalent vaccine has the advantages of stable quality, long immunity time, few adverse reactions, safety, and reliability. At the same time, the bivalent vaccine can simultaneously effectively control the canine distemper virus and canine parvovirus, and has a prominent immunity effect. The application dosage is equal to that of a mono-valent vaccine, and the effect of the bivalent vaccine is better than the total effect of two mono-valent vaccines. The vaccination procedure is simplified, the frequency of stress reactions of vaccinated animals is reduced, and one vaccine can prevent two diseases.

Description

technical field [0001] The invention belongs to the technical field of vaccines, in particular to a dual vaccine against canine distemper and canine parvovirus, and also relates to a preparation method for the dual vaccine against canine distemper and canine parvovirus. Background technique [0002] Canine distemper (CD) is an acute, highly contagious infectious disease caused by canine distemper virus (CDV) infection. The incidence of the disease is high, almost reaching 100%, and the clinical symptoms are diverse. Mixed infection of bacteria and viruses and secondary infection are prone to occur, and the mortality rate is as high as 80%. Young animals are mostly acute and fatal infections, while adult animals can be chronic persistent infections. The main clinical features are biphasic fever, mucous membrane catarrh, central nervous system symptoms and footpad swelling. The disease is distributed worldwide and is currently one of the most harmful diseases to the dog indus...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/23A61K9/19A61P31/20A61P31/14A61K39/155A61K31/7036A61K31/43
Inventor 范金红徐进李建正
Owner ZHENGZHOU HOUYI PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com