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Duck Tembusu virus envelope e protein specific binding peptide and its application

A duck Tembusu virus, specific technology for applications, antiviral agents, peptides, etc.

Inactive Publication Date: 2016-08-24
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For this disease, there is no report abroad

Method used

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  • Duck Tembusu virus envelope e protein specific binding peptide and its application
  • Duck Tembusu virus envelope e protein specific binding peptide and its application
  • Duck Tembusu virus envelope e protein specific binding peptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] In the present embodiment, the screening of duck Tembusu virus envelope E protein-specific binding peptide comprises the following steps:

[0021] 1) Preparation of DTMUV HN1 strain envelope E protein by conventional method

[0022] (1) Construction of recombinant expression plasmid pET32a-E

[0023] According to the gene sequence of the E protein of DTMUV HN1 strain published by GenBank (serial number: JQ669731), use DNAStar software to analyze, design a pair of specific primers P1 and P2, and add restriction enzyme sites EcoR I and Xba I to both ends of the primers respectively and protective bases (synthesized by Takara Company), the underlined part is the enzyme cleavage site.

[0024] Upstream primer P1: 5'-CCG GAATTC TTCAGCTGTCTGGGGATGCA-3' (such as SEQ ID NO: 2, the underlined part is the EcoR I restriction site);

[0025] Downstream primer P2: 5'-CGA TCTAGA ATTGACATTTACTGCCAGG-3' (such as SEQ ID NO: 3, the underlined part is the Xba I restriction site).

...

Embodiment 2

[0054] In this embodiment, the application of the specific binding peptide of the duck Tembusu virus envelope E protein in the preparation of drugs against duck Tembusu virus disease comprises the following steps: taking the duck Tembusu virus envelope E protein specific binding peptide The powder after purification and lyophilization (the specific binding peptide of duck Tembusu virus envelope E protein is synthesized by solid-phase peptide synthesis, purified by reverse high performance liquid chromatography, and the purity after purification and lyophilization reaches more than 95%), and medicine Commonly used auxiliary materials in the field are starch, sucrose, and talcum powder mixed to obtain a tablet drug that inhibits the proliferation of duck Tembusu virus. The specific binding peptide of duck Tembusu virus envelope E protein is used as the main antiviral active ingredient in antiviral drugs. The main drug The content of each tablet is 0.1g, and the percentage content...

Embodiment 3

[0056] In this example, the application of the duck Tembusu virus envelope E protein-specific binding peptide in the preparation of a feed additive for inhibiting the duck Tembusu virus proliferation comprises the following steps: specifically binding the duck Tembusu virus envelope E protein After the peptide is purified, it is freeze-dried to obtain a peptide powder with a purity of more than 95% (high performance liquid chromatography is used for purification, and freeze-drying method is used for freeze-drying). According to the feed weight, 0.2-0.5‰ duck Tembusu virus envelope E protein-specific binding peptide can be added, that is, 200-500 grams of peptide powder can be added per ton of feed.

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Abstract

The invention discloses a duck Tembusu virus envelope E protein-specific binding peptide and an application thereof, belonging to the technical field of animal virus molecular biology. The invention obtains duck tambushu virus envelope E protein-specific binding peptide by screening phage random 12 peptide library, and its amino acid sequence is: THRSWQGNSWYM. The specific binding peptide can inhibit the proliferation of duck tampusu virus HN1 strain in duck embryo fibroblasts, and can significantly down-regulate the virus copy number of duck tampusu virus in duck embryo fibroblasts at different concentrations, and at the same time significantly Decreases the viral titer of duck Tembusu virus in duck embryo fibroblasts, but itself has no significant effect on the proliferation of duck embryo fibroblasts. The duck tembusu virus envelope E protein-specific binding peptide has good application prospects in the preparation of drugs or feed additives against duck tembusu virus disease, and can be used for the prevention and treatment of duck tembusu virus disease.

Description

technical field [0001] The invention relates to a duck Tembusu virus envelope E protein specific binding peptide, and also relates to the application of the specific binding peptide, which belongs to the technical field of animal virus molecular biology. Background technique [0002] In recent years, a new viral infectious disease of ducks has broken out in southern China, mainly causing a serious decline in duck egg production. The disease spreads rapidly and involves a wide range. For paralysis, rolling, unsteady standing and ataxia, some sick ducks even died within a few days. Autopsy dead ducks, most of them showed obvious enlarged spleen and kidney, and laying ducks showed hemorrhagic oophoritis. The disease first broke out in Jiangsu and Zhejiang, then spread to Fujian and Anhui, and then successively broke out in Jiangxi, Shandong, Hunan, Henan, and Hebei. For this disease, foreign countries have not yet been reported. Domestic researchers have isolated the pathoge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A23K20/147A61P31/14
Inventor 王臣张才何雷牛明媚张春杰
Owner HENAN UNIV OF SCI & TECH
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