Automatic digital slide focusing device and method based on microscope

A digital slicing and auto-focusing technology, applied in microscopes, installations, optics, etc., can solve problems such as focus oscillations

Active Publication Date: 2014-12-03
杭州卓腾信息技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the fixed step size in this case can easily make the focus oscillate

Method used

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  • Automatic digital slide focusing device and method based on microscope
  • Automatic digital slide focusing device and method based on microscope
  • Automatic digital slide focusing device and method based on microscope

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Embodiment 1

[0052] Such as figure 1 As shown, the microscope-based digital slice automatic focusing device provided in this embodiment includes a trinocular microscope 1 and a computer 8. An automatic object loading platform 2, a scanning eyepiece 3 and a focusing knob 4 are installed on the trinocular microscope 1. The automatic object loading platform 2 is detachably installed on the trinocular microscope 1, and is used for moving along the Z-axis direction, and the repeated positioning accuracy along the Z-axis direction is 0.09um. The automatic object loading platform 2 is fixedly connected with a focusing sleeve 6; A high-speed area-scan digital camera 7 is installed on the scanning eyepiece 3, and is connected with a computer 8 by a USB3.0 connection line. The frame rate of the high-speed area-scan digital camera 7 is 120 frames; 6 is connected with the Z-axis motor 5; the Z-axis motor 5 is connected to the automatic loading platform controller 9 through the RS232 connection line, ...

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Abstract

The invention relates to an automatic digital slide focusing device and method based on a microscope. The automatic focusing device comprises the trinocular microscope and a computer, and the trinocular microscope is provided with an automatic objective table, a scanning ocular lens and a focusing turn button, wherein the automatic objective table can freely move along the axis-Z direction, the scanning ocular lens is provided with a digital camera that is connected with of the computer, and the focusing turn button plays the role of focusing. Clear visual images are obtained in the focusing method, namely an improved over-peaked step-variable hill-climbing focusing search method. The focusing device uses the high-precision automatic objective table, instruction is emitted by the computer, the automatic objective table is controlled to move along the axis-Z direction, the improved over-peaked step-variable hill-climbing focusing search method is used, the high-speed digital camera cooperates with the high-precision automatic objective table, the scanning time of the digital slide is reduced, influence of mechanical error on the focusing effect is reduced, the problem that a traditional step-fixed focusing method leads to oscillation is overcome, and the optimal focal plane can be rapidly and accurately determined.

Description

technical field [0001] The invention relates to the technical field of microscope autofocus, in particular to a microscope-based digital slice autofocus device and method thereof. Background technique [0002] In the process of digital slice imaging, focusing is a very critical step. The focusing effect directly affects the clarity of image imaging and the scanning quality of digital slices. [0003] The auto-focus method commonly used in microscopes mainly uses a climbing function to search for the best focus in the current field of view. Usually based on a field of view, a fixed-length moving Z-axis position captures several images, and then calculates the image clarity of these several different Z-level images. The plane of the image corresponding to the maximum image clarity is the best focus plane. This method is simple and effective, and can obtain the best focused image for each field of view. But the fixed step size in this case can easily make the focus oscillate....

Claims

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Application Information

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IPC IPC(8): G02B21/24G02B21/36G02B7/36
Inventor 叶志前姜磊王鑫鑫
Owner 杭州卓腾信息技术有限公司
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