Armeniaca vulgaris fermented extract and preparation method and application thereof
A technology of extract and apricot, which is applied in the field of tobacco flavors, can solve the problems of no application, etc., and achieve the effects of simple production process, convenient large-scale industrial production, strong standardization and controllability
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Embodiment 1
[0027] 1. Media and culture conditions
[0028] The Saccharomyces longans CGMCC 6645 used in the experiment was inoculated in 50 mL of YPD liquid medium, and cultured at 28°C and 160 r / min for 24 hours to obtain activated liquid strains.
[0029] 2. Preparation of Apricot Meat Samples
[0030] Slice the apricot pulp, dry it fully, crush it, and pass through a 40-mesh sieve. Weigh 20 g of apricot meat powder, add 400 mL of distilled water (20 volume times), sterilize, and set aside.
[0031] 3. Aroma-producing yeast fermentation treatment of apricot meat
[0032] Inoculate 8 mL of the activated Saccharomyces longiosa solution prepared in 1 into the apricot flesh sample prepared in 2 (the inoculum size is 2%), and culture with shaking at 28°C and 160r / min for 24 hours for the next experiment.
[0033] 4. Extraction of spice additives
[0034] After filtering the above fermented liquid, centrifuge at 8000r / min for 10 minutes, take the supernatant, concentrate it under reduced...
Embodiment 2
[0047] Repeat Example 1, with the following differences:
[0048] The culture conditions for the activation of the strain CGMCC 6645 are 28°C, 160r / min shaking culture, and the culture time is 24 hours; after the apricot meat is crushed, add 22 times of distilled water to sterilize; the activated bacteria solution is inoculated on the surface of the apricot meat material The inoculum amount is 2.5%; the fermentation and cultivation time after inoculation is 24 hours; the fermentation liquid supernatant is concentrated under reduced pressure at a temperature of 55° C.; the prepared spice additive is evenly sprayed on the shredded tobacco, and the addition amount is 12 g / box.
[0049] The results of flavoring and smoking test show that the flavor additive can harmonize with the aroma of cigarettes, improve the quality of aroma, increase the amount of aroma, improve the aftertaste, cover up the miscellaneous smell, and have a fresh and special aroma.
Embodiment 3
[0051] Repeat Example 1, with the following differences:
[0052] The culture conditions for the activation of the strain CGMCC 6645 are 30°C, 180r / min shaking culture, and the culture time is 12 hours; after the apricot meat is crushed, 15 times of distilled water is added to sterilize; the activated bacteria solution is inoculated on the surface of the apricot meat material The inoculum amount is 1.5%; the fermentation and cultivation time after inoculation is 12 hours; the supernatant of the fermentation broth is concentrated under reduced pressure at a temperature of 45° C.; the prepared spice additive is evenly sprayed on the shredded tobacco, and the addition amount is 8 g / box.
[0053] The results of flavoring and smoking test show that the flavor additive can harmonize with the aroma of cigarettes, improve the quality of aroma, increase the amount of aroma, improve the aftertaste, cover up the miscellaneous smell, and have a fresh and special aroma.
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