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Extraction method of hyoscyamine

An extraction method, the technology of scopolamine, applied in the field of phytochemistry, can solve the problems of large energy consumption, large environmental pollution, and high production cost of chemical synthesis

Inactive Publication Date: 2014-12-03
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are few literatures and patents about extracting hyoscyamine in our country. They mainly use chemical synthesis to prepare hyoscyamine. Chemical synthesis not only has high production cost, but also consumes a lot of energy and pollutes the environment.

Method used

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  • Extraction method of hyoscyamine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The fresh raw material was dried in an oven at 60° C. for 1 hour, crushed through a 40-mesh sieve, and the coarse powder was collected for later use. Weigh 20g of material coarse powder, add 0.3g of cellulase, and add 40ml of water to infiltrate, and enzymolyze it for 1h under the conditions of pH 4 and temperature 45°C to obtain a coarse powder enzymatic hydrolyzate. The hydrolyzate was placed in a Soxhlet extractor, 80ml of ethanol was added, and alcohol extraction was carried out using the principle of solvent reflux and siphon. The crude extract of scopolamine was obtained. The obtained hyoscyamine crude extract is eluted on a D101 type anion exchange resin column, first eluted with distilled water of 3 times the column volume, and then eluted with 4 times the column volume of ethanol, and the ethanol eluate is collected and concentrated under reduced pressure to 1 / 5 of the original volume, namely hyoscyamine. After testing, the content of scopolamine in the obtai...

Embodiment 2

[0026] The fresh raw materials were dried in an oven at 65°C for 2 hours, crushed through a 60-mesh sieve, and the coarse powder was collected for later use. Weigh 20g of material coarse powder, add 0.5g of hemicellulase, and add 80ml of water to infiltrate, enzymatically hydrolyze for 3 hours under the conditions of pH 5 and temperature 47°C to obtain the coarse powder enzymatic hydrolyzate, and the obtained coarse powder Put the enzymatic hydrolyzate in a Soxhlet extractor, add 100ml of ethanol, use the principle of solvent reflux and siphon to carry out alcohol extraction, first reflux the sample for 2 times, and finally soak it in the solvent for 22h, and then continue to extract for 2h , to obtain the crude extract of scopolamine. The obtained hyoscyamine crude extract is eluted on a D101 type anion exchange resin column, first eluted with distilled water of 4 times the column volume, and then eluted with 4 times the column volume of ethanol, and the ethanol eluate is col...

Embodiment 3

[0028] The fresh raw materials were dried in an oven at a temperature of 70° C. for 3 hours, crushed through a 60-mesh sieve, and the coarse powder was collected for later use. Weigh 20g of raw material powder, add 0.7g of pectinase, and add 100ml of water to infiltrate, and enzymolyze it for 4 hours at a pH of 5 and a temperature of 50°C to obtain an enzymatic hydrolyzate of the coarse powder. The hydrolyzate was placed in a Soxhlet extractor, 200ml of ethanol was added, and it was extracted by solvent reflux and siphon. The crude extract of scopolamine was obtained. The obtained hyoscyamine crude extract is eluted on a D101 type anion exchange resin column, first eluted with distilled water of 5 times the column volume, and then eluted with 10 times the column volume of ethanol, collecting the ethanol eluate, and concentrating under reduced pressure to 1 / 2 of the original volume, namely hyoscyamine. After testing, the content of scopolamine in the obtained sample was 71.62...

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Abstract

The invention discloses an extraction method of hyoscyamine and in particular relates to a method for Soxhlet and macroporous adsorption resin combined extraction and purification of hyoscyamine. The method mainly comprises the following steps: processing raw materials, performing biological enzymolysis, performing Soxhlet extraction, treating by a macroporous adsorption resin column, and concentrating to obtain hyoscyamine. The method is simple in process, high in extraction efficiency and easy for industrialized production.

Description

technical field [0001] The invention discloses a method for extracting hyoscyamine, in particular to a method for extracting and purifying hyoscyamine by combining Soxhlet extraction and macroporous adsorption resin, and belongs to the technical field of phytochemistry. Background technique [0002] Alkaloids are a type of nitrogen-containing basic organic compounds that exist in nature (mainly plants, but some also exist in animals). They have alkali-like properties, so they were also called pseudoalkali in the past. Most of them have a complex ring structure, and nitrogen is mostly contained in the ring, which has significant biological activity and is one of the important active ingredients in Chinese herbal medicine. Optically active. Some nitrogen-containing organic compounds derived from plants that are not alkaline have obvious biological activity, so they are still included in the scope of alkaloids. [0003] A tropane-type alkaloid (English name: Hyoscyamine), mol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D451/10
CPCC07D451/10
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
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