Method for determining contents of effective components in cefoperazone sodium and tazobactam sodium for injection

A technology of cefoperazone sodium and tazobactam sodium, which is applied in the field of medicine and biology, can solve the problems of high cost and trouble, and achieve the effect of low cost and effective quality control

Inactive Publication Date: 2014-11-26
SICHUAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing method for determining the content of the target substance in cefoperazone sodium and tazobactam sodium for injection is high performance liquid chromatography, but this method is cumbersome and expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The method for measuring active ingredient content in cefoperazone sodium tazobactam sodium for injection comprises the following steps:

[0021] (1) Prepare internal standard solution, cefoperazone sodium and tazobactam sodium test solution for injection, cefoperazone sodium reference solution, tazobactam sodium reference solution, the internal standard solution is hydrochlorothiazide solution;

[0022] (2) Weigh 69mg of cefoperazone sodium and tazobactam sodium for injection, dissolve it in a volumetric flask, place it in a 100mL volumetric flask, dissolve it ultrasonically with methanol and set the volume to the mark, shake well, draw 9mL, and place it in a 10mL volumetric flask. Add 1mL of internal standard solution and shake well, send to high-efficiency capillary electrophoresis instrument for sample injection, measure and analyze, and calculate the content of cefoperazone sodium and tazobactam sodium according to the standard curve method based on the relative pea...

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PUM

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Abstract

The invention discloses a method for determining contents of effective components in cefoperazone sodium and tazobactam sodium for injection. According to the method, a capillary electrophoresis method is used, and hydrochlorothiazide is taken as an interior label for quantitatively determining cefoperazone sodium and tazobactam sodium. The method for determining contents of effective components in cefoperazone sodium and tazobactam sodium for injection is relatively simple and quick, low in cost and capable of effectively controlling quality in the process of producing cefoperazone sodium and tazobactam sodium preparations for injection.

Description

[0001] technical field [0002] The invention relates to the field of medical biology, in particular to a method for determining the content of active ingredients in cefoperazone sodium and tazobactam sodium for injection. Background technique [0003] Cefoperazone is a third-generation cephalosporin antibiotic that achieves bactericidal effect by inhibiting the biosynthesis of sensitive bacterial cell walls. Tazobactam has no antibacterial activity against other bacteria except Neisseriaceae and Acinetobacter, but tazobactam has activity against most important β-lactamases produced by β-lactam antibiotic-resistant strains Irreversible inhibition. Tazobactam can prevent drug-resistant bacteria from destroying penicillins and cephalosporins, and tazobactam has obvious synergistic effects with penicillins and cephalosporins. Since tazobactam can bind to certain penicillin-binding proteins, sensitive strains may be more sensitive to this compound preparation than cefoperazone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 包莹刘萍陈继源
Owner SICHUAN PHARMA
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