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Primer for amplifying COI sequence of DNA code bar of viper species, PCR method and kit

A kit and a technology for amplifying products, applied in the directions of DNA/RNA fragments, DNA preparation, recombinant DNA technology, etc., can solve the problems that general primers LCO1490 and HCO2198 cannot obtain better amplified products, and achieve good versatility and DNA The effect of using less

Inactive Publication Date: 2014-11-26
晁志
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Problems solved by technology

At present, the universal primers recommended for COI sequence amplification are LCO1490 and HCO2198, but the inventors found in their research that universal primers LCO1490 and HCO2198 cannot obtain better amplification products in the identification of Viperidae species

Method used

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  • Primer for amplifying COI sequence of DNA code bar of viper species, PCR method and kit
  • Primer for amplifying COI sequence of DNA code bar of viper species, PCR method and kit
  • Primer for amplifying COI sequence of DNA code bar of viper species, PCR method and kit

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Embodiment Construction

[0020] The present invention will be described in further detail in conjunction with the following specific examples and accompanying drawings, and the protection content of the present invention is not limited to the following examples. Without departing from the spirit and scope of the inventive concept, changes and advantages conceivable by those skilled in the art are all included in the present invention, and the appended claims are the protection scope. The process, conditions, reagents, experimental methods, etc. for implementing the present invention are general knowledge and common knowledge in the art except for the content specifically mentioned below, and the present invention has no special limitation content.

[0021] 1. Materials

[0022] 10 Viperidae protozoans, their sources are detailed in (Table 1). All samples were identified by Zhang Liang, South China Institute of Endangered Animals.

[0023] Table 1 Viperidae samples

[0024]

[0025] 2. Universal ...

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Abstract

The invention belongs to the technical field of viper species and traditional Chinese herbal identification, and discloses a primer for amplifying the COI sequence of DNA code bar of viper species, a PCR method, and a kit; wherein the method comprises the steps of DNA extraction, PCR amplification, and electrophoresis detection. The identification method has the characteristics of convenience, little DNA using amount, and good universality; and overcomes the shortages of COI sequence universal primers (LCO1490 and HCO2198) in viper gene amplification and traditional Chinese herbal identification in the prior art. The primer can be applied to the amplification of COI sequence of viper, and establishes a foundation for molecular identification of viper.

Description

technical field [0001] The invention belongs to the technical field of identification of Viperidae snake species and Chinese medicinal materials, and in particular relates to a universal primer capable of amplifying the COI barcode sequence of Viperidae snake species, a PCR method and a kit. Background technique [0002] Snake medicinal materials have a long history of use in my country, which can be traced back to the Spring and Autumn Period and the Warring States Period BC. Herbal medicines of all dynasties, especially Li Shizhen's "Compendium of Materia Medica", have been extensively collected and accumulated, becoming the ancient herbal medicine that contains the most snake medicines. "Chinese Pharmacopoeia" (2010 edition) included 5 snakes including Viper (pentapod snake), black snake, money snake (coral snake) and snake slough (black snake, golden snake, black eyebrow snake) and so on. species, 4 herbs. [0003] Snakes are a population with a relatively fragile ecolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/53C12N15/10C12Q1/68
Inventor 晁志
Owner 晁志
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