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Detection method of dandelion and viola philippica granules

A granule and Qingpu technology, applied in the detection field of Qingpu granules, can solve the problems of interference of impurity components, large difference in peak area, difficulty in content determination, etc., and achieve the effect of suitable retention time and good peak shape.

Active Publication Date: 2014-11-19
CHENGDU QIANKUN VETERINARY PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Preliminary studies of this type of preparation have shown that when the two components of alfalfa and caffeic acid in Qingpu granules are determined simultaneously by using the high-performance liquid chromatography content determination method of viola dicin or dandelion, the chromatographic peaks of the two components are the same. Mutual interference, poor separation, serious interference of impurity components, large difference in peak area, difficult content determination
However, the current literature has also reported the content detection methods of acetaminophen and caffeic acid in other products, but these methods mostly use gradient elution, and their mobile phase procedures are relatively complicated.

Method used

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  • Detection method of dandelion and viola philippica granules
  • Detection method of dandelion and viola philippica granules
  • Detection method of dandelion and viola philippica granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 The detection method of Qingpu granule of the present invention

[0044] The preparation method of Qingpu granule of the present invention is as follows:

[0045] Prescription: 8 parts of Daqingye, 8 parts of Dandelion, 3 parts of Zihua Diding, 1 part of Licorice

[0046] Process: The above four herbs are decocted twice with water, adding 18 times the amount of water for the first time, and 15 times the amount of water for the second time, decocting for 1 hour each time, combining the decoctions, filtering, and concentrating the filtrate to relative density 1.30-1.35 (80°C), add appropriate amount of sucrose powder and starch, mix well, make granules, and dry to obtain.

[0047] The detection method is as follows:

[0048] Chromatographic conditions and system suitability experiment Shimadzu WondaCract ODS-2 (4.6×250mm, 5μm); methanol-0.6% glacial acetic acid aqueous solution (20:80) was used as the mobile phase; the detection wavelength was 326nm; the colu...

Embodiment 2

[0053] Embodiment 2 The detection method of Qingpu granule of the present invention

[0054] The preparation method of Qingpu granule of the present invention is as follows:

[0055] Prescription: 10 parts of Daqingye 10 parts of dandelion

[0056] Process: The above four herbs are decocted twice with water, adding 18 times the amount of water for the first time, and 15 times the amount of water for the second time, decocting for 1 hour each time, combining the decoctions, filtering, and concentrating the filtrate to relative density 1.30-1.35 (80°C), add appropriate amount of sucrose powder and starch, mix well, make granules, and dry to obtain.

[0057] The detection method is as follows:

[0058] Chromatographic conditions and system suitability experiment Shimadzu WondaCract ODS-2 (4.6×250mm, 5μm); methanol-0.6% glacial acetic acid aqueous solution (20:80) was used as the mobile phase; the detection wavelength was 326nm; the column temperature was 25°C. The number of th...

Embodiment 3

[0063] Embodiment 3 The detection method of Qingpu granule of the present invention

[0064] The preparation method of Qingpu granule of the present invention is as follows:

[0065] Prescription: 6 parts of Daqingye, 6 parts of Dandelion, 4 parts of Zihua Diding, 2 parts of Licorice

[0066] Process: The above four herbs are decocted twice with water, adding 18 times the amount of water for the first time, and 15 times the amount of water for the second time, decocting for 1 hour each time, combining the decoctions, filtering, and concentrating the filtrate to relative density 1.30-1.35 (80°C), add appropriate amount of sucrose powder and starch, mix well, make granules, and dry to obtain.

[0067] The detection method is as follows:

[0068] Chromatographic conditions and system suitability experiment Shimadzu WondaCract ODS-2 (4.6×250mm, 5μm); methanol-0.6% glacial acetic acid aqueous solution (20:80) was used as the mobile phase; the detection wavelength was 326nm; the c...

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Abstract

The invention provides a detection method of caffeic acid and aesculetin at the same time in a dandelion and viola philippica granules or an intermediate extract thereof. Through multiple experiment and research, chromatographic conditions of HPLC in detection of the two ingredients at the same time are screened and explored and a content determining method by which the two ingredients in the dandelion and viola philippica granules or the intermediate extract thereof can be detected at the same time. Under the chromatographic conditions, chromatographic peaks of the two ingredients can be separated successfully, interferences of impurity peaks are avoided, a retain time is proper and peak shapes are excellent. By means of the method, an object of successfully determining the contents of the two ingredients can be achieved and a basis of quality researching of a preparation is established. The detection method also provides a reference method of quantitative researching of a preparation containing the caffeic acid and the aesculetin at the same time.

Description

technical field [0001] The invention relates to a detection method of Qingpu granules. Background technique [0002] Veterinary clinical practice has proved that abnormal body temperature is one of the important symptoms of disturbed physiological functions of pigs. The normal body temperature of pigs is 38-40°C. If it exceeds 40°C continuously, it is fever, and prolonged high fever will damage the body. Large, first of all, it will accelerate the body's catabolism, excessive consumption of nutrients, and disordered digestive function, resulting in body weight loss and decreased resistance, and can also cause damage to the central nervous system and blood circulation system, causing depression in sick pigs, resulting in coma, or Serious consequences such as heart failure. A large number of modern studies have shown that a large number of traditional Chinese medicines have the functions of clearing away heat and detoxification, antibacterial and antiviral, and have their own...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 吴学渊房春林
Owner CHENGDU QIANKUN VETERINARY PHARMA
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