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Petroleum hydrocarbon degrading bacteria carrier material and preparation method and purpose thereof

A technology of petroleum hydrocarbon degrading bacteria and carrier materials, applied in the field of contaminated soil remediation

Inactive Publication Date: 2014-11-19
BINZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, people pay more attention to the first two conditions, and there are few reports on carrier materials that can create conditions for microbial degradation capabilities.

Method used

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  • Petroleum hydrocarbon degrading bacteria carrier material and preparation method and purpose thereof
  • Petroleum hydrocarbon degrading bacteria carrier material and preparation method and purpose thereof
  • Petroleum hydrocarbon degrading bacteria carrier material and preparation method and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation method of petroleum hydrocarbon degrading bacteria carrier material comprises the following steps:

[0026] 1) Cut the corn cob into 1cm 3 Rinse the left and right pieces with water, soak them in an aqueous hydrochloric acid solution with a volume concentration of 11% for 14 hours; take them out, wash them with sterile water until they are about neutral, dry them, pulverize them, and pass through a 20-mesh sieve to obtain corn cob powder; Soybean stalks are cut into 15cm sections, rinsed with water, soaked in 11% hydrochloric acid aqueous solution for 14 hours; taken out, rinsed with sterile water until neutral, dried, pulverized, and passed through a 20-mesh sieve to obtain soybeans straw powder;

[0027] 2) The corn cob powder and the soybean straw powder were mixed according to the mass ratio of 2:1 to prepare the carrier material of the petroleum hydrocarbon degrading bacteria.

Embodiment 2

[0029] The preparation method of petroleum hydrocarbon degrading bacteria carrier material comprises the following steps:

[0030] 1) Cut the corn cob into 1.5cm 3 Rinse the left and right blocks with water, and soak them in 12% aqueous hydrochloric acid solution for 12 hours; take them out, rinse them with sterile water until they are neutral, dry them, pulverize them, and pass through a 20-mesh sieve to obtain corn cob powder; The straw is cut into 20cm sections, rinsed with water, soaked in 12% hydrochloric acid aqueous solution for 12 hours; taken out, rinsed with sterile water until neutral, dried, pulverized, and passed through a 20-mesh sieve to obtain soybean straw powder ;

[0031] 2) The corn cob powder and the soybean straw powder were mixed according to the mass ratio of 2:1 to prepare the carrier material of the petroleum hydrocarbon degrading bacteria.

Embodiment 3

[0033] The preparation method of petroleum hydrocarbon degrading bacteria carrier material comprises the following steps:

[0034] 1) Cut the corn cob into 2cm 3 Rinse the left and right blocks with water, and soak them in 10% hydrochloric acid aqueous solution for 16 hours; take them out, rinse them with sterile water until they are neutral, dry them, pulverize them, and pass through a 20-mesh sieve to obtain corn cob powder; The straw is cut into 10cm sections, rinsed with water, soaked in hydrochloric acid aqueous solution with a volume concentration of 10% for 16 hours; taken out, rinsed with sterile water until neutral, dried; pulverized, passed through a 20-mesh sieve to obtain soybean straw powder ;

[0035] 2) The corn cob powder and the soybean straw powder were mixed according to the mass ratio of 2:1 to prepare the carrier material of the petroleum hydrocarbon degrading bacteria.

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Abstract

The invention discloses a petroleum hydrocarbon degrading bacteria carrier material and the purpose thereof. A preparation method is as follows: 1) cubing the corncobs, rinsing with water, and soaking in an aqueous solution of hydrochloric acid; taking out the corncobs, washing by sterile water to a neutral state, drying, crushing, sieving through a screen of 20 mesh to obtain a corncob powder; cutting soybean straws into segments, rinsing with water, and soaking in an aqueous solution of hydrochloric acid; taking out the soybean straw segments, washing by sterile water to a neutral state, drying, crushing, and sieving through a screen of 20 mesh to obtain a soybean straw powder; and 2) mixing the corncob powder and soybean straw powder according to the ratio of 2:1 to prepare the petroleum hydrocarbon degrading bacteria carrier material. Compared with the traditional carrier materials, the petroleum hydrocarbon degrading bacteria carrier material provided by the invention can significantly reduce repellency water and surface tension of soil, improve water holding capacity and microbial activity of petroleum polluted soil, create good conditions for the hydrocarbon degradation ability of the bacteria, and significantly improve the degradation efficiency.

Description

technical field [0001] The invention belongs to the field of contaminated soil remediation, and in particular relates to a carrier material of petroleum hydrocarbon degrading bacteria, a preparation method and application. Background technique [0002] Soil oil pollution is an important environmental problem in my country's oil producing areas. After the soil is polluted by oil, the productivity will decrease, and the harmful components in the oil can also enter the water body, the atmosphere, and even agricultural products, seriously affecting the environmental quality and endangering human health. Experts from various countries have been conducting research and development and tackling key problems in the field of oil-contaminated soil remediation technology. However, due to the complexity of oil components, once it pollutes the environment, it is more difficult to restore. Bioremediation is a very potential approach for oil-contaminated soil treatment, which mainly inclu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/12B09C1/10C12R1/425C12R1/01
Inventor 谢文军张衍鹏吴涛王君刘少文
Owner BINZHOU UNIV
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