Eyedrop and preparation method thereof
An eye drop and solution technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, peptide/protein components, etc., can solve problems such as affecting the ocular surface microenvironment of patients, exacerbating ocular surface diseases, and aplastic anemia. , to achieve the effect of reducing adverse reactions, reducing complications and sequelae, and simple ingredients
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Embodiment 1
[0043] Based on 10mL eye drops, the base solution is 7.4mL PPBS, containing fetal bovine serum 200 μ L. Low-molecular-weight dextran 100 μL, HEPES 200 μL, tobramycin 100 μL, UNC5B recombinant protein 2 mL, pH value 7.2-7.4, osmotic pressure 350-380 mOsm / L.
[0044] Preparation method 1: Dissolve 1 μg UNC5B recombinant protein and 100 μg low-molecular-weight dextran in 2 mL of PBS solution, then mix with 200 μL of fetal bovine serum, 200 μL of 1M HEPES and 100 μL of 10% tobramycin, add PBS solution to 10 mL, and adjust the pH value to 7.2 -7.4, the osmotic pressure is 350-380mOsm / L, and sterilized by filtration through a 0.2μm membrane to obtain UNC5B eye drops.
[0045] Preparation method 2: Make 1 μg UNC5B recombinant protein into sterile micropowder, add 100 μg low-molecular-weight dextran, 200 μL fetal bovine serum, 200 μL 1M HEPES, 100 μL 10% tobramycin and 9.4 mL LPBS, adjust the pH value to 7.2-7.4, and pass through 0.2 μm Sterilize by membrane filtration, and then diss...
Embodiment 2
[0047]Based on 10mL of eye drops, 7.4mL of HBSS as the base solution, containing 200μL of fetal bovine serum, 100μL of low molecular weight dextran, 200μL of HEPES, 100μL of tobramycin, 2mL of UNC5B recombinant protein, pH value of 7.2-7.4, osmotic pressure of 350~ 380mOsm / L.
[0048] Preparation method 1: Dissolve 1 μg of UNC5B recombinant protein and 100 μg of low-molecular dextran in 2 mL of HBSS solution, then mix with 200 μL of fetal bovine serum, 200 μL of 1M HEPES and 100 μL of 10% tobramycin, add HBSS solution to 10 mL, and adjust the pH value to 7.2 -7.4, the osmotic pressure is 350-380mOsm / L, and sterilized by filtration through a 0.2μm membrane to obtain UNC5B eye drops.
[0049] Preparation method 2: Make 1 μg of UNC5B into sterile micropowder, add 100 μg of low-molecular dextran, 200 μL of fetal bovine serum, 200 μL of 1M HEPES, 100 μL of 10% tobramycin and 9.4 mL of HBSS, adjust the pH value to 7.2-7.4, and filter through a 0.2 μm membrane to remove Bacteria, an...
Embodiment 3
[0051] Based on 10 mL of eye drops, 7.4 mL of normal saline is used as the basic solution, containing 200 μL of fetal bovine serum, 100 μL of low-molecular dextran, 200 μL of HEPES, 100 μL of tobramycin, and 2 mL of UNC5B recombinant protein. The pH value is 7.2-7.4, and the osmotic pressure is 350~380mOsm / L.
[0052] Preparation method 1: Dissolve 1 μg of UNC5B recombinant protein and 100 μg of low-molecular dextran in 2 mL of normal saline solution, then mix well with 200 μL of fetal bovine serum, 200 μL of 1M HEPES and 100 μL of 10% tobramycin, add normal saline solution to 10 mL, and adjust the pH The value is 7.2-7.4, the osmotic pressure is 350-380mOsm / L, and the UNC5B eye drops are obtained by filtering and sterilizing through a 0.2μm membrane.
[0053] Preparation method 2: Make 1 μg of UNC5B into sterile micropowder, mix 100 μg of low-molecular-weight dextran, 200 μL of fetal bovine serum, 200 μL of 1M HEPES, 100 μL of 10% tobramycin and 9.4 mL of normal saline, adjus...
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