Eyedrop and preparation method thereof

An eye drop and solution technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, peptide/protein components, etc., can solve problems such as affecting the ocular surface microenvironment of patients, exacerbating ocular surface diseases, and aplastic anemia. , to achieve the effect of reducing adverse reactions, reducing complications and sequelae, and simple ingredients

Inactive Publication Date: 2014-11-19
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In addition, most ophthalmic drugs currently used clinically have limited therapeutic functions, and there may be factors that influence and restrict each other in various drug components. The damage to the surface microenvironment makes the existing ocular surface diseases worse, and brings certain confusion to the treatment of various corneal diseases; in addition, most anti-inflammatory products contain hormone components, and long-term use can seriously affect the patient's ocular surface. Surface microenvironment, some anti-inflammatory drugs and related complications such as large doses of chloramphenicol eye drops can cause eyelid and corneal edema, restricted eye movement and optic disc atrophy, long-term use can cause aplastic anemia, etc.; Mymycin eye drops are currently an effective drug for treating inflammation, but there is no official commercialized preparation in my country at present

Method used

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  • Eyedrop and preparation method thereof
  • Eyedrop and preparation method thereof
  • Eyedrop and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Based on 10mL eye drops, the base solution is 7.4mL PPBS, containing fetal bovine serum 200 μ L. Low-molecular-weight dextran 100 μL, HEPES 200 μL, tobramycin 100 μL, UNC5B recombinant protein 2 mL, pH value 7.2-7.4, osmotic pressure 350-380 mOsm / L.

[0044] Preparation method 1: Dissolve 1 μg UNC5B recombinant protein and 100 μg low-molecular-weight dextran in 2 mL of PBS solution, then mix with 200 μL of fetal bovine serum, 200 μL of 1M HEPES and 100 μL of 10% tobramycin, add PBS solution to 10 mL, and adjust the pH value to 7.2 -7.4, the osmotic pressure is 350-380mOsm / L, and sterilized by filtration through a 0.2μm membrane to obtain UNC5B eye drops.

[0045] Preparation method 2: Make 1 μg UNC5B recombinant protein into sterile micropowder, add 100 μg low-molecular-weight dextran, 200 μL fetal bovine serum, 200 μL 1M HEPES, 100 μL 10% tobramycin and 9.4 mL LPBS, adjust the pH value to 7.2-7.4, and pass through 0.2 μm Sterilize by membrane filtration, and then diss...

Embodiment 2

[0047]Based on 10mL of eye drops, 7.4mL of HBSS as the base solution, containing 200μL of fetal bovine serum, 100μL of low molecular weight dextran, 200μL of HEPES, 100μL of tobramycin, 2mL of UNC5B recombinant protein, pH value of 7.2-7.4, osmotic pressure of 350~ 380mOsm / L.

[0048] Preparation method 1: Dissolve 1 μg of UNC5B recombinant protein and 100 μg of low-molecular dextran in 2 mL of HBSS solution, then mix with 200 μL of fetal bovine serum, 200 μL of 1M HEPES and 100 μL of 10% tobramycin, add HBSS solution to 10 mL, and adjust the pH value to 7.2 -7.4, the osmotic pressure is 350-380mOsm / L, and sterilized by filtration through a 0.2μm membrane to obtain UNC5B eye drops.

[0049] Preparation method 2: Make 1 μg of UNC5B into sterile micropowder, add 100 μg of low-molecular dextran, 200 μL of fetal bovine serum, 200 μL of 1M HEPES, 100 μL of 10% tobramycin and 9.4 mL of HBSS, adjust the pH value to 7.2-7.4, and filter through a 0.2 μm membrane to remove Bacteria, an...

Embodiment 3

[0051] Based on 10 mL of eye drops, 7.4 mL of normal saline is used as the basic solution, containing 200 μL of fetal bovine serum, 100 μL of low-molecular dextran, 200 μL of HEPES, 100 μL of tobramycin, and 2 mL of UNC5B recombinant protein. The pH value is 7.2-7.4, and the osmotic pressure is 350~380mOsm / L.

[0052] Preparation method 1: Dissolve 1 μg of UNC5B recombinant protein and 100 μg of low-molecular dextran in 2 mL of normal saline solution, then mix well with 200 μL of fetal bovine serum, 200 μL of 1M HEPES and 100 μL of 10% tobramycin, add normal saline solution to 10 mL, and adjust the pH The value is 7.2-7.4, the osmotic pressure is 350-380mOsm / L, and the UNC5B eye drops are obtained by filtering and sterilizing through a 0.2μm membrane.

[0053] Preparation method 2: Make 1 μg of UNC5B into sterile micropowder, mix 100 μg of low-molecular-weight dextran, 200 μL of fetal bovine serum, 200 μL of 1M HEPES, 100 μL of 10% tobramycin and 9.4 mL of normal saline, adjus...

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Abstract

The invention discloses an eyedrop which has the pH value of 7.2-7.4 and comprise the following components in percentage by volume: 10%-15% of UNC5B recombinant protein, 0.1%-5% of bovine serum, 5%-15% of a thickener, 1%-5% of a pH regulator and 0.5%-2% of antibiotics; a solvent is a balanced salt solution. A preparation method of the eye drops is simple, reliable and easy to perform; the eye drops are simple in component, relatively low in cost and convenient to prepare.

Description

technical field [0001] The invention specifically relates to an eye drop and a preparation method thereof. Background technique [0002] Corneal disease is one of the common blinding eye diseases. Clinically, corneal inflammation and corneal damage caused by various factors can cause corneal defect, erosion and ulcer. If not treated effectively, it will seriously damage the visual function of the patient and quality of life. In corneal diseases, keratitis is a common disease in ophthalmic diseases. At present, the commonly used drugs for the clinical treatment of non-specific inflammation in ophthalmology are steroid hormone eye drops and non-steroidal anti-inflammatory eye drops. Steroid hormone eye drops have a definite curative effect, but long-term topical application of steroid drugs can easily cause ophthalmic complications such as cataracts and glaucoma. When non-steroidal anti-inflammatory eye drops are used, local eyes often have burning and stinging sensations, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K9/08A61P27/02A61K35/16
Inventor 韩云李炜刘祖国邵毅王贺刘婷婷张丽颖
Owner XIAMEN UNIV
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