Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Quality detection method of compound donkey-hide gelatin slurry oral liquid

A quality testing method and technology for compound donkey-hide glue, which can be applied to measuring devices, instruments, scientific instruments, etc., and can solve the problems of unreasonable quality of traditional Chinese medicine compound preparations and no quantitative testing method for active chemical components.

Active Publication Date: 2014-11-05
SHAN DONG DONG E E JIAO
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, at present, the quality standard detection method of compound donkey-hide gelatin pulp oral liquid is limited to total flavonoids, total saponins and other indicators, there is no quantitative detection method for active chemical components, and it cannot fully reflect the quality of the product at all. The quality is not very reasonable; and the competition of similar products in the market is becoming increasingly fierce, so in order to protect the interests of patients, it is of great significance to establish a detection method for the active ingredients in compound donkey-hide gelatin oral liquid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quality detection method of compound donkey-hide gelatin slurry oral liquid
  • Quality detection method of compound donkey-hide gelatin slurry oral liquid
  • Quality detection method of compound donkey-hide gelatin slurry oral liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The quality detection of embodiment 1 Chinese medicine compound donkey-hide gelatin slurry oral liquid

[0047] 1. Experimental materials

[0048] (1) Preparation of the test solution: Take 5 batches of compound donkey-hide gelatin pulp oral liquid (110950, 1109511, 110952, 110953, 110954), remove the protein in the donkey-hide gelatin pulp according to the sample pretreatment method described in the technical plan, and centrifuge , static, centrifugal concentration, dilution and other steps for analysis;

[0049] (2) Preparation of standard products: Accurately weigh different amounts of chlorogenic acid, ergosteroside, isergosteroside, tangshenoside, baicalin, notoginsenoside R2, 20(S)-ginsenoside Rg2, 20( R)-ginsenoside Rg2, 20(S)-ginsenoside Rg3, and 20(R)-ginsenoside Rg3 were formulated into a mixed standard solution as shown in the table below.

[0050] Table 1 Standard solution (μg / mL)

[0051]

[0052]

[0053] (3) Preparation of internal standard solut...

Embodiment 2

[0067] Embodiment 2 Sensitivity experiment

[0068] Sensitivity experiments include the sensitivity of the instrument and the sensitivity of the method, which are expressed by the detection limit of the instrument and the quantification limit of the method, respectively.

[0069] Dilute HB-6 with 80% methanol-water 10 times, 30 times, 60 times, 120 times, 240 times, 360 times, 480 times respectively, according to the sample pretreatment method, add the dilution of HB-6 to the blank gel In the juice, after centrifugation, standing still, centrifugal concentration, dilution and other steps, sample analysis was carried out to determine the signal-to-noise ratio. The quantitation limit of the method.

Embodiment 3

[0070] Embodiment 3 accuracy and repeatability experiment

[0071] Take 4 sticks of compound donkey-hide gelatin oral liquid from the same batch and mix them. After ultrasonication for 20 minutes, accurately pipette 1 mL of oral liquid and 1 mL of blank gum juice. 40 μL of internal standard solution was processed according to the standard operation, and the sample was injected for analysis, and the recovery rate and relative standard deviation were calculated; the accuracy was expressed by the recovery rate, and the repeatability was expressed by the relative standard deviation.

[0072] For the sample pretreatment method and analysis method, to investigate the influence of the matrix on the analysis results, it is necessary to determine the extraction efficiency and matrix effect of the sample pretreatment. Three sets of standard stock solutions of high, medium and low concentrations were prepared respectively, and the recovered samples and matrix samples were prepared by add...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a quality detection method of a compound donkey-hide gelatin slurry oral liquid. The method adopts a high performance liquid chromatography (HPLC) which is serially connected to a triple quadrupole mass spectrum (MS) to carry out on-line detection on four medicinal materials and ten compounds. The optimal mobile phase is selected through a large amount of experiments, and the gradient elution program and flow speed, MS conditions, and related compound parameters are also determined. Through the observation of methodology, the quality detection method has the advantages of good stability, selectivity, and repeatability and reliable analysis result. Moreover the sensitivity of the method is high, and the quantitative and qualitative analysis on a compound donkey-hide gelatin slurry oral liquid can be well achieved by the method. The quality of a compound donkey-hide gelatin slurry oral liquid can be objectively, comprehensively, and precisely evaluated, and the method has a very important meaning on controlling the quality of compound donkey-hide gelatin slurry oral liquid and guaranteeing the curative effect in clinic.

Description

technical field [0001] The invention relates to a quality detection method of a compound traditional Chinese medicine, in particular to a quality detection method of a compound donkey-hide gelatin slurry oral liquid. Background technique [0002] The compound donkey-hide gelatin oral liquid is a compound traditional Chinese medicine oral liquid produced by Shandong Dong'e E-jiao Co., Ltd., which is made of donkey-hide gelatin, red ginseng, Codonopsis pilosula, Rehmannia glutinosa and hawthorn. The functions of the liver and kidney are mainly used for deficiency of Qi and blood, dizziness, palpitation, insomnia, loss of appetite and anemia. [0003] The compound donkey-hide gelatin slurry oral liquid is made from the following raw materials, 5 parts of donkey-hide gelatin, 6 parts of red ginseng, 8 parts of rehmannia glutinosa, 12 parts of Codonopsis pilosula, and 5 parts of hawthorn. The preparation method of making the above-mentioned components into the medicine of the pre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 瞿海斌周祥山田守生王春艳沈丽娟李民张路李士栋
Owner SHAN DONG DONG E E JIAO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com