Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Citrus greening disease rapid detection method

A citrus huanglongbing and detection method technology, which is applied in the preparation of test samples, analysis through chemical reaction of materials, and material analysis through observation of the impact on chemical indicators, can solve the impact judgment, freezing and decolorization time Long time, long time and other problems, to achieve the effect of ensuring integrity, short decolorization time and maintaining consistency

Inactive Publication Date: 2014-11-05
GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (1) Refrigeration is required, although every household has a refrigerator now, some farmers are unwilling to use household refrigerators for freezing citrus leaves;
[0009] (2) The time of freezing and decolorization is long, resulting in too long detection cycle;
[0010] (3) The leaves need to be cut into thin strips of about 1 mm during sample processing, which is difficult for users to grasp during operation
When the thin strip is too wide, the color will be decolorized and the dyeing time will be prolonged, and it will often cause only the cut incision to develop color, which will affect the judgment;
[0011] (4) The test results cannot be released in time, and some farmers hope to see the results in the field without taking them home for experiments;
[0012] (5) The time for freezing and decolorization is too long, it takes 6-10 hours, and when the temperature is low, the time is longer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Citrus greening disease rapid detection method
  • Citrus greening disease rapid detection method
  • Citrus greening disease rapid detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Cover the citrus leaves to be detected with a black bag, seal the seal, and perform dark treatment for 12 hours to obtain dark-treated citrus leaves to be detected. Select Huanglongbing infection leaf (symptomatic leaf), Huanglongbing infection leaf (asymptomatic leaf) and normal health leaf three kinds of blades therefrom respectively, every kind of blade is divided into two halves, handles with following method respectively:

[0034] 1. Cut the citrus leaves to be tested into thin strips about 1mm wide, put them in the refrigerator to freeze at -10°C, take them out after 24 hours and put them in chlorophyll decolorization solution (acetone) for decolorization, after 10 hours, the thin strips of leaves become For white leaves, pour off the decolorizing solution, add starch color developing solution (iodine) to develop color, observe the change of leaf color for diagnosis, if the leaves turn blue, it means that the citrus leaves to be tested are leaves infected by Huangl...

Embodiment 2

[0041] In a sugar orange orchard in Yunan County, on May 28, 2014, the citrus leaves to be tested were covered with black bags, sealed, and dark-treated for 24 hours to obtain dark-treated citrus leaves to be tested. Gently grind the citrus leaves to be tested with glass sandpaper to grind off the waxy layer on the surface of the leaves, put the leaves in chlorophyll decolorization solution (acetone) for 2 hours for decolorization, the leaves turn white, pour off the chlorophyll decolorization solution , add starch color solution (iodine) to the leaves after decolorization, and observe the color change of the leaves. If the leaves turn blue, it means that the citrus leaves to be tested are leaves infected with Huanglongbing. If the leaves do not change color, they are normal leaves. . A color change is found after 1 minute, such as figure 2 As shown, it can be seen that the citrus leaves to be tested are citrus Huanglongbing infected leaves. It is the same as the detection ...

Embodiment 3

[0043] In a sugar orange orchard in Yunan County, at 3 o'clock in the afternoon on May 28, 2014, the citrus leaves to be tested were covered with black bags, sealed, and the dark treatment was completed. Sampling was completed at 11:30 on the 29th, with a total of 50 samples Tree. The citrus leaves to be tested (cut from the same piece) were divided into two processing methods.

[0044] 1. Gently polish the citrus leaves to be tested with glass sandpaper to remove the waxy layer on the surface of the leaves. Put the leaves into the chlorophyll decolorization solution (acetone) for 2 hours to decolorize the leaves. The leaves turn white and pour off the chlorophyll. Decoloring solution, drip starch color developing solution (iodine) on the leaves after decolorization, observe the color change of the leaves, if the leaves turn blue, it means that the citrus leaves to be detected are leaves infected by Huanglongbing, if the leaves do not change color, it is normal leaves. A col...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a citrus greening disease rapid detection method. The method comprises the following steps: step a, polishing citrus leaves which have been subjected to a dark treatment to get rid of the wax layer on the leaf surfaces, soaking the leaves in a chlorophyll decoloring liquid until the leaves turn white; step b, color development detection: dropwise adding a starch color developing solution on the discolored leaves, and observing the color change of the leaves, if the leaves turn blue, the citrus leaves must be from a citrus tree affected by citrus greening disease, and if the leaves do not change color, the leaves are healthy. The method has the advantages of more convenient operation, shorter time, and more precise result, and can be operated by farmers themselves.

Description

Technical field: [0001] The invention belongs to the field of citrus disease prevention and control, and in particular relates to a rapid detection method for citrus huanglongbing. Background technique: [0002] Citrus huanglongbing disease (HLB) is a destructive disease of citrus that can occur throughout the year, and symptoms can appear in spring, summer and autumn shoots. Generally, there are three types of yellowing of leaves: uniform yellowing, mottled yellowing and deficiency yellowing. In the diseased plants, some new leaves may start to chlorosis and yellowing from the base of the leaves, near the veins or edges, and gradually expand into yellow and green mottled yellowing, and this mottled yellowing may also turn into uniform yellowing; some New shoots are issued on the yellowed branches, some yellowed branches are cut and new shoots are pulled out, the branches are short and the leaves are small and hard, and they also show symptoms of zinc deficiency and mangane...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N1/28
Inventor 张宇宏郭洋洋郑基焕毛润乾
Owner GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products