Nickel-titanium alloy containing bioactive coating and its preparation method and application

A nickel-titanium alloy and bioactive technology, applied in the field of preparation of the alloy, can solve the problems that a single bioactive molecule is difficult to simulate the microenvironment outside the endothelial cells, and cannot meet the rapid endothelialization of the material surface, so as to promote adhesion and growth , increase long-term effectiveness, and solve the effect of inducing thrombosis

Inactive Publication Date: 2016-01-13
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complexity of the natural microenvironment system of cells, the existing methods that only involve the introduction of a single bioactive molecule on the surface of implanted materials are difficult to simulate the extracellular microenvironment of endothelial cells, and thus cannot meet the needs of rapid endothelialization of the material surface

Method used

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  • Nickel-titanium alloy containing bioactive coating and its preparation method and application
  • Nickel-titanium alloy containing bioactive coating and its preparation method and application
  • Nickel-titanium alloy containing bioactive coating and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take a nickel-titanium alloy sheet and follow the steps below to construct a biologically active coating imitating the extracellular microenvironment:

[0029] (1) Substrate pretreatment

[0030] Polish the nickel-titanium alloy sheet with 100-mesh, 300-mesh and 2000-mesh sandpaper in turn until the surface is bright, and ultrasonically clean it with acetone for 5 minutes to obtain a clean metal surface;

[0031] (2) deposition polydopamine film layer

[0032] With 10mM, the Tris buffer solution that pH value is 8.5 prepares the dopamine hydrochloride solution of 2mg / mL, soaks the nickel-titanium alloy after step (1) treatment in this solution for 24 hours, obtains the nickel-titanium alloy of surface deposition polydopamine film layer ;

[0033] (3) Layer-by-layer self-assembly

[0034] With the acetic acid of 0.3% (v / v) as solvent, prepare the chitosan solution of 5mg / mL; Prepare the gelatin solution of 5mg / mL and the endothelial cell growth factor solution of 200n...

Embodiment 2

[0037] Step (2) of this embodiment uses 20 mM Tris buffer solution with a pH value of 7.2 to prepare a 1 mg / mL dopamine hydrochloride solution, and soaks the nickel-titanium alloy treated in step (1) in the solution for 12 hours to obtain a surface deposited polymer. Nitinol alloy for dopamine film layer;

[0038] Present embodiment step (3) is solvent with the acetic acid of 0.5% (v / v), the chitosan solution of preparation 1mg / mL; The endothelial cell growth factor solution of mL; The nickel-titanium alloy of step (2) deposition polydopamine film layer is soaked in gelatin solution 36 hours, then immerses chitosan solution, gelatin solution, endothelial cell growth factor solution and gelatin solution successively, Soak in each solution for 20 minutes, and wash with ultrapure water for 1 minute after each soak;

[0039] Soak chitosan solution, gelatin solution, endothelial cell growth factor solution and gelatin solution in sequence as a layer-by-layer self-assembly cycle, a...

Embodiment 3

[0042] Step (2) of this embodiment uses 20 mM Tris buffer solution with a pH value of 9 to prepare a 3 mg / mL dopamine hydrochloride solution, and soaks the nickel-titanium alloy treated in step (1) in the solution for 36 hours to obtain a surface deposited polymer. Nitinol alloy for dopamine film layer;

[0043] Present embodiment step (3) present embodiment step (3) is solvent with the acetic acid of 0.5% (v / v), the chitosan solution of preparation 10mg / mL; The gelatin solution of mL and the endothelial cell growth factor solution of 500ng / mL; The nickel-titanium alloy of step (2) deposition polydopamine film layer is immersed in gelatin solution for 12 hours, then immerses chitosan solution, gelatin solution, endothelial cell successively Growth factor solution and gelatin solution, soak in each solution for 5 minutes, wash with ultrapure water for 1 minute after each soak;

[0044] Soak chitosan solution, gelatin solution, endothelial cell growth factor solution and gelati...

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Abstract

The invention discloses a nickel-titanium alloy containing a biologically-active coating. A preparation method for the alloy comprises the following steps: polishing the surface of the nickel-titanium alloy so as to allow the surface of the nickel-titanium alloy to be shining; then soaking the alloy in a Tris-dopamine hydrochloride solution with a concentration of 1 to 3 mg / mL for 12 to 36 h so as to allow a polydopamine film to be formed on the surface; preparing a chitosan solution with a concentration of 1 to 10 mg / mL with 0.1 to 0.5% (v / v) acetic acid as a solvent, preparing a gelatin solution with a concentration of 1 to 10 mg / mL and an endothelial cell growth factor solution with a concentration of 100 to 500 ng / mL by using a phosphate buffer solution, soaking the treated nickel-titanium alloy in the gelatin solution for 12 to 36 h and then carrying out self-assembling of chitosan-gelatin-endothelial cell growth factor-gelatin layer by layer, wherein soaking in each solution lasts for 5 to 20 min and there are 4 to 10 cycles of layer-by-layer self-assembling; and finally, carrying out blow-drying with nitrogen. The coating can effectively promote adhesion and growth of endothelial cells on the surface of the nickel-titanium alloy, so demands of rapid endothelialization of the nickel-titanium alloy as a cardiovascular implant material are met, the problems of induction of thrombi and generation of coagulation on the surface of the nickel-titanium alloy are overcome, and the clinical application scope of the nickel-titanium alloy is broadened.

Description

technical field [0001] The invention relates to metal materials, in particular to a nickel-titanium alloy containing a bioactive coating, and also to a preparation method and application of the alloy. Background technique [0002] In recent years, nickel-titanium alloy materials have been widely used as long-term implant devices in the cardiovascular field, such as cardiovascular stents and cardiac occluder etc. However, the clinical application of nickel-titanium alloys is still limited due to the problems that the surface of the material is easy to induce thrombus and blood coagulation. [0003] Endothelial cells have specific antithrombotic properties, and promoting the rapid growth and coating of endothelial cells on the surface of materials can effectively reduce coagulation problems. The microenvironment of endothelial cells plays an important role in regulating cell growth behavior. In recent years, those skilled in the art have used various methods to construct th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B32B5/14B32B9/00B32B33/00C09D105/08C09D189/00C09D7/12A61L27/06A61L27/34A61L27/54C23C26/00
Inventor 刘鹏赵永春
Owner CHONGQING UNIV
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