A method for constructing the core population of carp disease-resistant families

A carp and family technology, which is applied in fish farming, climate change adaptation, animal husbandry, etc., can solve the problems of being easily restricted by family, heavy workload, time-consuming, labor-intensive and difficult, and achieve stable inheritance of disease resistance and reduce work The effect of cycle time and workload reduction

Active Publication Date: 2016-01-06
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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Problems solved by technology

[0003] The present invention aims to solve the problems existing in the existing molecular-assisted disease-resistant breeding technology in the process of disease-resistant breeding in fish. For example, the QTL mapping method needs to obtain disease-resistant / susceptible families and have a large number of molecular markers, and the screened out Relevant markers are often limited by family pedigrees, which is time-consuming, labor-intensive, and heavy workload. For example, the candidate gene method lacks relevant genome information, and the candidate genes required for research cannot be accurately determined. It is still necessary to screen and screen from a large amount of data, and the workload is heavy. Time-consuming, laborious and difficult problems, and provide a method to construct the core group of carp disease-resistant families

Method used

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  • A method for constructing the core population of carp disease-resistant families
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specific Embodiment approach 1

[0016] Specific implementation mode one: the construction of the core population of the carp disease-resistant family in this embodiment is carried out according to the following steps:

[0017] 1. Mating of carp broodstock;

[0018] 2. Divide the progeny of the same family into N groups, take one of them for infection experiment, and infect with pathogens; take the other group as blank control group;

[0019] 3. Extract the genome DNA of the offspring carp that survived the infection experiment, and the offspring carp genome DNA of the blank control group;

[0020] 4. The genomic DNA extracted in step 3 is amplified by PCR with primers;

[0021] 5. PCR data statistics, comparing the infection experimental group with the blank control group, determining the chromosomes in the infection experimental group that deviate from the Hardy-Weinberg equilibrium site and the genotypes on the chromosomes that are higher than the value of the law of gene segregation;

[0022] 6. Based o...

specific Embodiment approach 2

[0028] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 1, the broodstock is paired one-to-one. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0029] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 1, broodstock are free to mate. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention relates to a method for breeding a disease-resistant family core group of carps and solves the problem that an existing molecular-assistance disease-resistant breeding technology is high in workload, time and labor wasting and high in difficulty in the disease-resistant breeding process. The method for breeding the disease-resistant family core group of carps includes mating of parent fishes, infection experiment, genomic DNA extraction, PCR (polymerase chain reaction) augmentation, PCR data statistics and screening on the basis of data, accordingly, a disease-resistant family core group of carps is obtained. By screening and positioning through well-known immunogene polymorphism microsatallite markers, operation efficiency and reliability are greatly improved, and limit by families is rejected. The bred disease-resistant family is stable in inheritance of disease resistance and is used for breeding the disease-resistant family of the carps.

Description

technical field [0001] The invention relates to a method for constructing a carp family core group. Background technique [0002] With the increase of people's demand for aquatic products, the problem of fish disease in aquaculture is becoming more and more significant, and has gradually become a bottleneck for the sustainable and stable development of the aquaculture industry. Breeding and using disease-resistant varieties is the most economical and effective measure to prevent and control diseases. Molecular assisted breeding for disease resistance has become the main method of disease resistance breeding. At present, the molecular-assisted disease resistance breeding technology in fish mainly adopts QTL mapping and candidate gene method. However, the QTL mapping method needs to obtain disease-resistant / susceptible families and a large number of molecular markers, and the screened related markers are mostly restricted by the family and related to statistical methods, whic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01K61/00
CPCY02A40/81
Inventor 贾智英石连玉赵新春李池陶葛彦龙胡雪松
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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