Method for culturing agrocybe aegerita through litchi branch crumbs
A technology for lychee chips and tea mushrooms, applied in the directions of botanical equipment and methods, applications, fertilizer mixtures, etc., can solve the problems of polluting the environment, reducing the cultivation cost of tea mushrooms, waste of waste materials, etc., so as to avoid pollution, reduce Cultivation cost, low price effect
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[0040] A. Preparation of liquid strains
[0041] First-class liquid culture medium formula: 20% potato, 3% bran, 2% glucose, KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 1 10mg / L, CMC0.3%, water 1000mL, pH natural;
[0042] Secondary liquid culture medium formula: 5% litchi powder, 20% potato, 3% bran, 2% glucose, KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 1 10mg / L, CMC0.3%, water 1000mL, natural pH; among them, lychee crumbs were crushed and sieved through an 80-mesh sieve to obtain a powder, boiled with potatoes and bran for 30 minutes, filtered to remove slag to obtain an extract.
[0043]The first-level liquid strains are prepared in a 500mL conical flask, with a liquid volume of 200ml in each bottle. After autoclaving at 121°C for 30 minutes, insert PDA slant strains under sterile conditions, and the inoculation volume of each bottle is 4 pieces of 1cm 2 strains. After standing still for 24 hours after inoculation, put the Erlenmeyer flask on a shaker at 160r / min, and incubate at a co...
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